+Open data
-Basic information
Entry | Database: PDB / ID: 7oa8 | ||||||
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Title | PilC minor pilin of Streptococcus sanguinis SK36 type IV pili | ||||||
Components | PilC minor pilin | ||||||
Keywords | STRUCTURAL PROTEIN / pilin | ||||||
Function / homology | establishment of competence for transformation / Prokaryotic N-terminal methylation site. / Prokaryotic N-terminal methylation motif / Prokaryotic N-terminal methylation site / Concanavalin A-like lectin/glucanase domain superfamily / membrane => GO:0016020 / Uncharacterized protein Function and homology information | ||||||
Biological species | Streptococcus sanguinis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.6 Å | ||||||
Authors | Sheppard, D. / Pelicic, V. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2023 Title: Characterization of a glycan-binding complex of minor pilins completes the analysis of Streptococcus sanguinis type 4 pili subunits. Authors: Shahin, M. / Sheppard, D. / Raynaud, C. / Berry, J.L. / Gurung, I. / Silva, L.M. / Feizi, T. / Liu, Y. / Pelicic, V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7oa8.cif.gz | 171.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7oa8.ent.gz | 128.2 KB | Display | PDB format |
PDBx/mmJSON format | 7oa8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oa/7oa8 ftp://data.pdbj.org/pub/pdb/validation_reports/oa/7oa8 | HTTPS FTP |
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-Related structure data
Related structure data | 7o5yC 7oa7SC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 51515.570 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus sanguinis (bacteria) / Gene: D8882_11420 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A3R9NIL7 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.98 Å3/Da / Density % sol: 37.81 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.2 M magnesium chloride hexahydrate; 20 % w/v polyethylene glycol 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jun 30, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→48.01 Å / Num. obs: 54911 / % possible obs: 100 % / Redundancy: 6.4 % / CC1/2: 0.999 / Net I/σ(I): 17.2 |
Reflection shell | Resolution: 1.6→1.63 Å / Num. unique obs: 2647 / CC1/2: 0.865 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 7OA7.pdb Resolution: 1.6→48.01 Å / Cross valid method: FREE R-VALUE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Displacement parameters | Biso mean: 43.08 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→48.01 Å
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Refine LS restraints |
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