German Federal Ministry for Education and Research
031B050B
Germany
Austrian Science Fund
P31001-B29
Austria
German Research Foundation (DFG)
INST 335/588-1
Germany
German Research Foundation (DFG)
INST 335/589-1
Germany
German Research Foundation (DFG)
INST 335/590-1
Germany
German Research Foundation (DFG)
HA 2549/15-2
Germany
Citation
Journal: Acta Crystallogr D Struct Biol / Year: 2022 Title: CryoEM analysis of small plant biocatalysts at sub-2 Å resolution. Authors: Nicole Dimos / Carl P O Helmer / Andrea M Chánique / Markus C Wahl / Robert Kourist / Tarek Hilal / Bernhard Loll / Abstract: Enzyme catalysis has emerged as a key technology for developing efficient, sustainable processes in the chemical, biotechnological and pharmaceutical industries. Plants provide large and diverse ...Enzyme catalysis has emerged as a key technology for developing efficient, sustainable processes in the chemical, biotechnological and pharmaceutical industries. Plants provide large and diverse pools of biosynthetic enzymes that facilitate complex reactions, such as the formation of intricate terpene carbon skeletons, with exquisite specificity. High-resolution structural analysis of these enzymes is crucial in order to understand their mechanisms and modulate their properties by targeted engineering. Although cryo-electron microscopy (cryoEM) has revolutionized structural biology, its applicability to high-resolution structural analysis of comparatively small enzymes has so far been largely unexplored. Here, it is shown that cryoEM can reveal the structures of plant borneol dehydrogenases of ∼120 kDa at or below 2 Å resolution, paving the way for the rapid development of new biocatalysts that can provide access to bioactive terpenes and terpenoids.
Mass: 30284.529 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salvia rosmarinus (rosemary) / Production host: Escherichia coli BL21(DE3) (bacteria)
Average exposure time: 30 sec. / Electron dose: 40 e/Å2 / Detector mode: COUNTING / Film or detector model: FEI FALCON III (4k x 4k) / Num. of grids imaged: 1 / Num. of real images: 1666
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