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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 7nmx | ||||||
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タイトル | Crystal structure of 14-3-3 sigma in complex with 13mer Amot-p130 peptide and fragment 12 | ||||||
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![]() | SIGNALING PROTEIN / protein-peptide complex fragment soaking | ||||||
機能・相同性 | ![]() establishment of cell polarity involved in ameboidal cell migration / cell migration involved in gastrulation / positive regulation of embryonic development / Regulation of CDH11 function / blood vessel endothelial cell migration / establishment of epithelial cell polarity / angiostatin binding / hippo signaling / gastrulation with mouth forming second / negative regulation of vascular permeability ...establishment of cell polarity involved in ameboidal cell migration / cell migration involved in gastrulation / positive regulation of embryonic development / Regulation of CDH11 function / blood vessel endothelial cell migration / establishment of epithelial cell polarity / angiostatin binding / hippo signaling / gastrulation with mouth forming second / negative regulation of vascular permeability / Signaling by Hippo / cell-cell junction assembly / regulation of small GTPase mediated signal transduction / regulation of epidermal cell division / protein kinase C inhibitor activity / positive regulation of epidermal cell differentiation / keratinocyte development / keratinization / Regulation of localization of FOXO transcription factors / endocytic vesicle / keratinocyte proliferation / positive regulation of cell size / phosphoserine residue binding / Activation of BAD and translocation to mitochondria / bicellular tight junction / negative regulation of keratinocyte proliferation / positive regulation of blood vessel endothelial cell migration / vasculogenesis / establishment of skin barrier / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / negative regulation of angiogenesis / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / stress fiber / negative regulation of stem cell proliferation / RHO GTPases activate PKNs / positive regulation of stress fiber assembly / ruffle / protein sequestering activity / protein kinase A signaling / negative regulation of innate immune response / protein export from nucleus / regulation of cell migration / TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest / release of cytochrome c from mitochondria / positive regulation of protein export from nucleus / stem cell proliferation / Translocation of SLC2A4 (GLUT4) to the plasma membrane / actin filament / TP53 Regulates Metabolic Genes / negative regulation of protein kinase activity / chemotaxis / intrinsic apoptotic signaling pathway in response to DNA damage / cell junction / protein localization / lamellipodium / signaling receptor activity / positive regulation of cell growth / actin cytoskeleton organization / cytoplasmic vesicle / angiogenesis / in utero embryonic development / regulation of cell cycle / cadherin binding / external side of plasma membrane / protein kinase binding / negative regulation of transcription by RNA polymerase II / cell surface / signal transduction / extracellular space / extracellular exosome / nucleoplasm / identical protein binding / membrane / nucleus / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() | ||||||
![]() | Centorrino, F. / Ottmann, C. | ||||||
資金援助 | European Union, 1件
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![]() | ![]() タイトル: Fragment-based exploration of the 14-3-3/Amot-p130 interface. 著者: Centorrino, F. / Andlovic, B. / Cossar, P. / Brunsveld, L. / Ottmann, C. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 130.5 KB | 表示 | ![]() |
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PDB形式 | ![]() | 82.1 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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1 | ![]()
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単位格子 |
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Components on special symmetry positions |
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要素
-タンパク質 / タンパク質・ペプチド , 2種, 2分子 AP
#1: タンパク質 | 分子量: 28226.518 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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#2: タンパク質・ペプチド | 分子量: 1626.817 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) ![]() |
-非ポリマー , 5種, 237分子 ![](data/chem/img/K7Q.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/CL.gif)
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#3: 化合物 | ChemComp-K7Q / ~{ | ||||||
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#4: 化合物 | #5: 化合物 | #6: 化合物 | ChemComp-MG / | #7: 水 | ChemComp-HOH / | |
-詳細
研究の焦点であるリガンドがあるか | Y |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.48 Å3/Da / 溶媒含有率: 50.44 % |
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結晶化 | 温度: 277.15 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: 0.095 M Hepes pH 7.5, 26% PEG 400, 0.19 M CaCl2 and 5% Glycerol. |
-データ収集
回折 | 平均測定温度: 100 K / Serial crystal experiment: N |
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放射光源 | 由来: SEALED TUBE / タイプ: RIGAKU MICROMAX-003 / 波長: 1.5419 Å |
検出器 | タイプ: DECTRIS PILATUS 200K / 検出器: PIXEL / 日付: 2018年3月26日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5419 Å / 相対比: 1 |
反射 | 解像度: 2.3→33.15 Å / Num. obs: 12756 / % possible obs: 97.1 % / 冗長度: 3.2 % / Biso Wilson estimate: 8.45 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.041 / Net I/σ(I): 18.3 |
反射 シェル | 解像度: 2.3→2.36 Å / Rmerge(I) obs: 0.064 / Mean I/σ(I) obs: 12.5 / Num. unique obs: 925 / CC1/2: 0.993 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: 4JC3 解像度: 2.3→29.89 Å / SU ML: 0.213 / 交差検証法: FREE R-VALUE / σ(F): 1.53 / 位相誤差: 21.6736 / 立体化学のターゲット値: GeoStd + Monomer Library
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 12.83 Å2 | ||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2.3→29.89 Å
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拘束条件 |
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LS精密化 シェル |
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