分子量: 29408.084 Da / 分子数: 2 / 由来タイプ: 組換発現 詳細: The expressed protein corresponds to residues 46-284 of UniProtKB - G8N1K9. However, sequence alignments indicate that Met46 in this sequence represents the true start and thus the numbering ...詳細: The expressed protein corresponds to residues 46-284 of UniProtKB - G8N1K9. However, sequence alignments indicate that Met46 in this sequence represents the true start and thus the numbering used in this PDB entry reflects this i.e Met46 is now Met1. The C-terminal sequence KLAAALEHHHHHH is a nickel affinity tag from the pET21b expression plasmid. 由来: (組換発現) Geobacillus thermoleovorans CCB_US3_UF5 (バクテリア) 遺伝子: noc, GTCCBUS3UF5_39100 / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): BL21(DE3) / Variant (発現宿主): Rosetta / 参照: UniProt: G8N1K9
構造決定の手法: 単波長異常分散 / 解像度: 2.5→84.89 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.934 / SU B: 13.604 / SU ML: 0.149 / SU R Cruickshank DPI: 0.223 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.223 / ESU R Free: 0.196 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
Rfactor
反射数
%反射
Selection details
Rfree
0.2399
1794
4.9 %
RANDOM
Rwork
0.2095
-
-
-
obs
0.2111
34834
99.95 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK