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Yorodumi- PDB-7n3y: Crystal Structure of Saccharomyces cerevisiae Apn2 Catalytic Doma... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7n3y | ||||||
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Title | Crystal Structure of Saccharomyces cerevisiae Apn2 Catalytic Domain E59Q/D222N Mutant in Complex with DNA | ||||||
Components |
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Keywords | HYDROLASE/DNA / nuclease / DNA complex / EEP fold / APE2 homolog / HYDROLASE / HYDROLASE-DNA complex | ||||||
Function / homology | Function and homology information double-stranded DNA 3'-5' DNA exonuclease activity / exodeoxyribonuclease III / phosphoric diester hydrolase activity / DNA-(apurinic or apyrimidinic site) endonuclease activity / 3'-5' exonuclease activity / base-excision repair / endonuclease activity / DNA binding / zinc ion binding / nucleus Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.73 Å | ||||||
Authors | Wojtaszek, J.L. / Krahn, J. / Wallace, B.D. / Williams, R.S. | ||||||
Funding support | United States, 1items
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Citation | Journal: Cell Rep / Year: 2022 Title: Molecular basis for processing of topoisomerase 1-triggered DNA damage by Apn2/APE2. Authors: Williams, J.S. / Wojtaszek, J.L. / Appel, D.C. / Krahn, J. / Wallace, B.D. / Walsh, E. / Kunkel, T.A. / Williams, R.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7n3y.cif.gz | 658.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7n3y.ent.gz | 538.5 KB | Display | PDB format |
PDBx/mmJSON format | 7n3y.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7n3y_validation.pdf.gz | 564.1 KB | Display | wwPDB validaton report |
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Full document | 7n3y_full_validation.pdf.gz | 590.1 KB | Display | |
Data in XML | 7n3y_validation.xml.gz | 55.1 KB | Display | |
Data in CIF | 7n3y_validation.cif.gz | 73.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n3/7n3y ftp://data.pdbj.org/pub/pdb/validation_reports/n3/7n3y | HTTPS FTP |
-Related structure data
Related structure data | 7n3zC 3g0rS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-Protein , 1 types, 4 molecules ABCD
#1: Protein | Mass: 47322.094 Da / Num. of mol.: 4 / Mutation: E59Q,D222N Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: APN2, ETH1, YBL019W, YBL0443 / Production host: Escherichia coli (E. coli) References: UniProt: P38207, Hydrolases; Acting on ester bonds |
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-DNA chain , 2 types, 4 molecules EFGH
#2: DNA chain | Mass: 4046.927 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #3: DNA chain | | Mass: 4055.940 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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-Non-polymers , 6 types, 107 molecules
#4: Chemical | #5: Chemical | ChemComp-CA / | #6: Chemical | #7: Chemical | ChemComp-CIT / | #8: Chemical | ChemComp-EDO / | #9: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.49 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.2M sodium citrate, 20% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 105 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å |
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Jul 24, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.73→36.97 Å / Num. obs: 51375 / % possible obs: 96.8 % / Redundancy: 3.3 % / Biso Wilson estimate: 67.86 Å2 / Rrim(I) all: 0.085 / Net I/σ(I): 18.4 |
Reflection shell | Resolution: 2.73→2.83 Å / Mean I/σ(I) obs: 1.44 / Num. unique obs: 4598 / Rrim(I) all: 0.891 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3G0R Resolution: 2.73→36.97 Å / SU ML: 0.333 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 23.917 Stereochemistry target values: GEOSTD + MONOMER LIBRARY + CDL V1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 101.5 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.73→36.97 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 5.1379 Å / Origin y: 20.3876 Å / Origin z: 32.6817 Å
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Refinement TLS group | Selection details: ALL |