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Open data
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Basic information
Entry | Database: PDB / ID: 7my6 | ||||||
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Title | Se-CrtE C-term His-tag with IPP added | ||||||
![]() | Farnesyl-diphosphate synthase | ||||||
![]() | TRANSFERASE / Prenyl transferase / Isopentenyl pyrophosphate / Dimethylallyl pyrophosphate / cyanobacteria / terpenoids | ||||||
Function / homology | ![]() (2E,6E)-farnesyl diphosphate synthase / isoprenoid biosynthetic process / geranyltranstransferase activity Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Peat, T.S. / Newman, J. | ||||||
Funding support | 1items
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![]() | ![]() Title: Molecular characterization of cyanobacterial short-chain prenyltransferases and discovery of a novel GGPP phosphatase. Authors: Satta, A. / Esquirol, L. / Ebert, B.E. / Newman, J. / Peat, T.S. / Plan, M. / Schenk, G. / Vickers, C.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 134.2 KB | Display | ![]() |
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PDB format | ![]() | 98.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 803.4 KB | Display | ![]() |
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Full document | ![]() | 806.8 KB | Display | |
Data in XML | ![]() | 24.1 KB | Display | |
Data in CIF | ![]() | 34.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7mxzSC ![]() 7my0C ![]() 7my1C ![]() 7my7C S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 33592.273 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: PCC 7942 / FACHB-805 / Gene: Synpcc7942_0776 / Production host: ![]() ![]() References: UniProt: Q31Q61, (2E,6E)-farnesyl diphosphate synthase |
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-Non-polymers , 7 types, 264 molecules ![](data/chem/img/IPE.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-IPE / | ||||||||||
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#3: Chemical | #4: Chemical | #5: Chemical | ChemComp-SO4 / #6: Chemical | ChemComp-MG / #7: Chemical | ChemComp-CL / | #8: Water | ChemComp-HOH / | |
-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.44 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 9.5 Details: 200 nL of protein at 10 mg/mL was added to 200 nL of reservoir consisting of 90 mM CHES pH 9.5, 180 mM magnesium sulfate, 100 mM trisodium citrate, 22.5% (w/v) PEG 1500 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 21, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9537 Å / Relative weight: 1 |
Reflection | Resolution: 1.94→48.21 Å / Num. obs: 45579 / % possible obs: 99.9 % / Redundancy: 10 % / CC1/2: 0.986 / Rmerge(I) obs: 0.345 / Rpim(I) all: 0.113 / Net I/σ(I): 5.7 |
Reflection shell | Resolution: 1.94→1.99 Å / Redundancy: 9.7 % / Rmerge(I) obs: 2.327 / Mean I/σ(I) obs: 1.1 / Num. unique obs: 3076 / CC1/2: 0.392 / Rpim(I) all: 0.781 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 7mxz Resolution: 2.02→45.482 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.932 / WRfactor Rfree: 0.189 / WRfactor Rwork: 0.163 / SU B: 5.122 / SU ML: 0.134 / Average fsc free: 0.9021 / Average fsc work: 0.9103 / Cross valid method: FREE R-VALUE / ESU R: 0.182 / ESU R Free: 0.15 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.581 Å2
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Refinement step | Cycle: LAST / Resolution: 2.02→45.482 Å
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Refine LS restraints |
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LS refinement shell |
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