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- PDB-7ms2: Three-dimensional structure of a GH3 Beta-glucosidase from Clostr... -

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Basic information

Entry
Database: PDB / ID: 7ms2
TitleThree-dimensional structure of a GH3 Beta-glucosidase from Clostridium thermocellum in complex with glycerol
ComponentsThermostable beta-glucosidase B
KeywordsHYDROLASE
Function / homology
Function and homology information


: / beta-glucosidase / beta-glucosidase activity / cellulose catabolic process
Similarity search - Function
: / Fibronectin type III-like domain / Fibronectin type III-like domain / Fibronectin type III-like domain / Glycoside hydrolase, family 3, active site / Glycosyl hydrolases family 3 active site. / Glycoside hydrolase family 3 C-terminal domain / Glycoside hydrolase, family 3, N-terminal domain / Glycoside hydrolase family 3 C-terminal domain / Glycosyl hydrolase family 3 C-terminal domain ...: / Fibronectin type III-like domain / Fibronectin type III-like domain / Fibronectin type III-like domain / Glycoside hydrolase, family 3, active site / Glycosyl hydrolases family 3 active site. / Glycoside hydrolase family 3 C-terminal domain / Glycoside hydrolase, family 3, N-terminal domain / Glycoside hydrolase family 3 C-terminal domain / Glycosyl hydrolase family 3 C-terminal domain / Glycoside hydrolase family 3 C-terminal domain superfamily / Glycoside hydrolase, family 3, N-terminal / Glycoside hydrolase, family 3, N-terminal domain superfamily / Glycosyl hydrolase family 3 N terminal domain / Glycoside hydrolase superfamily / TIM Barrel / Alpha-Beta Barrel / Immunoglobulin-like fold / Immunoglobulins / Immunoglobulin-like / Sandwich / Rossmann fold / 3-Layer(aba) Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
BETA-MERCAPTOETHANOL / Thermostable beta-glucosidase B
Similarity search - Component
Biological speciesHungateiclostridium thermocellum AD2 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.04 Å
AuthorsAlmeida, L.R. / Muniz, J.R.C.
Funding support Brazil, 1items
OrganizationGrant numberCountry
Sao Paulo Research Foundation (FAPESP)2016/09152-6 Brazil
CitationJournal: To Be Published
Title: Three-dimensional structure of a GH3 Beta-glucosidase from Clostridium thermocellum in complex with glycerol
Authors: Almeida, L.R. / Muniz, J.R.C.
History
DepositionMay 10, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 18, 2022Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Thermostable beta-glucosidase B
B: Thermostable beta-glucosidase B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)168,86411
Polymers168,1772
Non-polymers6889
Water7,260403
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: SAXS
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7130 Å2
ΔGint-32 kcal/mol
Surface area51210 Å2
MethodPISA
Unit cell
Length a, b, c (Å)63.840, 148.060, 198.640
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 3 through 5 or (resid 6...
21(chain B and ((resid 3 through 4 and (name N...

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11VALVALILEILE(chain A and (resid 3 through 5 or (resid 6...AA3 - 53 - 5
12LYSLYSLYSLYS(chain A and (resid 3 through 5 or (resid 6...AA66
13METMETLYSLYS(chain A and (resid 3 through 5 or (resid 6...AA1 - 7501 - 750
14METMETLYSLYS(chain A and (resid 3 through 5 or (resid 6...AA1 - 7501 - 750
15METMETLYSLYS(chain A and (resid 3 through 5 or (resid 6...AA1 - 7501 - 750
16METMETLYSLYS(chain A and (resid 3 through 5 or (resid 6...AA1 - 7501 - 750
17METMETLYSLYS(chain A and (resid 3 through 5 or (resid 6...AA1 - 7501 - 750
21VALVALASPASP(chain B and ((resid 3 through 4 and (name N...BB3 - 43 - 4
22VALVALLYSLYS(chain B and ((resid 3 through 4 and (name N...BB3 - 7503 - 750
23VALVALLYSLYS(chain B and ((resid 3 through 4 and (name N...BB3 - 7503 - 750
24VALVALLYSLYS(chain B and ((resid 3 through 4 and (name N...BB3 - 7503 - 750
25VALVALLYSLYS(chain B and ((resid 3 through 4 and (name N...BB3 - 7503 - 750

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Components

#1: Protein Thermostable beta-glucosidase B / Beta-D-glucoside glucohydrolase / Cellobiase / Gentiobiase / Beta-glucosidase 3B


Mass: 84088.375 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: Ser 738 is phosphorylated probably due to the chemical environment or buffer as post-translational modification
Source: (gene. exp.) Hungateiclostridium thermocellum AD2 (bacteria)
Strain: AD2 / Gene: bglB, Cthe_1256 / Plasmid: pET28 / Production host: Escherichia coli (E. coli) / References: UniProt: P14002, beta-glucosidase
#2: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C3H8O3
#3: Chemical ChemComp-BME / BETA-MERCAPTOETHANOL


Mass: 78.133 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C2H6OS
#4: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cl
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 403 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.79 Å3/Da / Density % sol: 55.97 % / Description: plate
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: 0.1 M Tris-HCl:Bicine; pH 8.5; 0.12 M Alcohols [1,6-Hexanediol (HEZ), 1-buthanol (1BO), 1,2-Propanediol (PGO), 2-Propanol (IPA), 1,4-Butanediol (BU1), 1,3-Propandiol (PDO)]; 37.5% v/v ...Details: 0.1 M Tris-HCl:Bicine; pH 8.5; 0.12 M Alcohols [1,6-Hexanediol (HEZ), 1-buthanol (1BO), 1,2-Propanediol (PGO), 2-Propanol (IPA), 1,4-Butanediol (BU1), 1,3-Propandiol (PDO)]; 37.5% v/v Precipitant Mix [25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350]
PH range: 7.0-8.5

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I24 / Wavelength: 0.96863 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Sep 10, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.96863 Å / Relative weight: 1
ReflectionResolution: 2.04→69.37 Å / Num. obs: 120455 / % possible obs: 99.8 % / Redundancy: 26.5 % / Biso Wilson estimate: 25.19 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.321 / Rpim(I) all: 0.063 / Rrim(I) all: 0.327 / Net I/σ(I): 7.8 / Num. measured all: 3197927 / Scaling rejects: 2
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique obsCC1/2Rpim(I) allRrim(I) all% possible all
2.04-2.1526.99.99173590.5962.87315.00399.6
6.45-69.3723.50.05741530.9990.0120.05899.9

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Phasing

PhasingMethod: molecular replacement
Phasing MRModel details: Phaser MODE: MR_AUTO
Highest resolutionLowest resolution
Rotation4.06 Å69.37 Å
Translation4.06 Å69.37 Å

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Processing

Software
NameVersionClassification
PHENIX1.19.2-4158refinement
xia2data reduction
Aimlessdata scaling
PHASER2.7.17phasing
PDB_EXTRACT3.27data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2X40

2x40


Resolution: 2.04→60.44 Å / SU ML: 0.24 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 27.83 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2297 1999 2.89 %
Rwork0.2017 67153 -
obs0.2025 69152 57.33 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 137.86 Å2 / Biso mean: 42.6041 Å2 / Biso min: 14.1 Å2
Refinement stepCycle: final / Resolution: 2.04→60.44 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11327 0 40 405 11772
Biso mean--42.34 32.42 -
Num. residues----1494
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00211565
X-RAY DIFFRACTIONf_angle_d0.50915672
X-RAY DIFFRACTIONf_dihedral_angle_d12.1664189
X-RAY DIFFRACTIONf_chiral_restr0.0421767
X-RAY DIFFRACTIONf_plane_restr0.0042053
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A6798X-RAY DIFFRACTION5.699TORSIONAL
12B6798X-RAY DIFFRACTION5.699TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.04-2.090.3231500.28941687173720
2.09-2.150.2811560.27861878193423
2.15-2.210.3426630.26772117218026
2.21-2.280.2819760.27172534261031
2.28-2.360.297920.27443123321538
2.36-2.460.3091080.26363619372744
2.46-2.570.33541190.25423976409548
2.57-2.710.28371340.26534499463354
2.71-2.880.29571550.26095209536462
2.88-3.10.27411790.24495985616472
3.1-3.410.27732090.22297001721084
3.41-3.90.20382440.18498215845998
3.9-4.920.1752530.146885268779100
4.92-60.440.18252610.1787849045100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.91886.50081.32917.4392.48086.19560.2753-0.1006-0.7149-0.07280.1564-1.0665-0.02060.4263-0.23240.6453-0.3227-0.12340.48450.16430.467935.086734.32782.5124
20.8533-0.50440.05984.7691-0.46530.4455-0.06610.14350.1766-0.1584-0.0898-0.1077-0.4634-0.00940.12110.5476-0.143-0.12010.28130.05640.201120.349224.7114.1102
36.4045-2.6561-2.04688.83126.34734.5918-0.12-0.45951.1525-0.4892-0.14880.3872-1.2543-1.01540.31350.8059-0.0516-0.05070.5369-0.14250.5586.950811.41793.9389
42.9957-0.05542.70491.5355-1.18345.4093-0.14980.1621-0.0469-0.4385-0.0608-0.20170.27220.33450.13670.2639-0.09220.04880.171-0.00220.138125.3308-1.123311.2224
50.90420.02870.0641.3697-0.5311.4908-0.10170.02270.0939-0.0678-0.0208-0.175-0.31990.37190.11260.2851-0.1072-0.04390.23610.03540.151526.970912.306320.1052
61.3072-0.0088-0.06253.74480.71881.9031-0.0822-0.01610.3946-0.2872-0.16850.2105-0.5180.07070.21520.404-0.1575-0.09950.2270.03790.231720.361427.759815.5657
71.4244-1.5522-1.14627.31593.12722.38330.19320.09520.3512-0.4534-0.0705-0.3983-0.53750.188-0.15220.4537-0.1764-0.05970.29450.09350.276928.332431.658.9356
83.98320.18750.80861.67331.43941.34370.05610.60870.1668-0.9731-0.19980.06680.2335-0.00040.11890.7148-0.06070.14430.2898-0.01140.168425.54860.358-3.7928
91.19270.2358-0.00960.88590.18370.7955-0.1250.0755-0.2781-0.22680.05890.09620.8598-0.08790.05270.6614-0.0819-0.00610.2744-0.00050.217819.1845-14.183412.9764
104.2351-0.8026-0.69254.44771.00860.2931-0.23380.1303-0.68470.0441-0.06790.16360.6075-0.18780.20061.2123-0.1375-0.07090.2851-0.01560.338916.7601-24.48735.1471
113.48353.2688-0.13718.2459-2.52611.1150.2267-0.3310.08170.8771-0.2080.82020.2767-0.20810.10170.9831-0.5011-0.06910.54830.03510.55083.003-21.799719.5516
120.66330.05310.27385.266-0.64860.207-0.0345-0.1285-0.0402-0.7825-0.0040.67360.347-0.45530.05280.2813-0.1214-0.03720.3290.03450.300311.7012-7.844922.2283
133.96630.091-1.15987.1776-0.5010.3702-0.1337-0.3659-0.540.5216-0.23980.17870.55510.00680.33260.8449-0.288-0.03290.36910.07180.379810.6314-21.933525.8379
140.7989-0.06290.20332.1661-0.02772.2785-0.0397-0.0808-0.13280.0175-0.0337-0.1150.47030.13330.05050.2898-0.00620.00940.21110.05640.13325.7914-7.344427.993
152.3778-0.5974-0.28272.6365-0.46173.0053-0.0789-0.0561-0.14750.1406-0.084-0.69010.52610.93240.10250.35070.11250.05740.51390.13410.351644.2407-5.727526.0241
160.55060.4363-0.31681.66170.580.7134-0.1245-0.1541-0.06310.322-0.0105-0.5430.00520.9046-0.17350.3182-0.0979-0.15970.74640.13390.390744.50483.856934.765
171.61371.4223.78955.25111.75699.58390.22960.0193-0.11910.6662-0.123-0.6986-0.50331.3886-0.11780.6482-0.2201-0.05940.67490.10180.482447.16319.910453.5188
184.50661.9589-0.34055.9416-0.68571.8607-0.3197-0.55090.26580.2524-0.0297-1.4805-0.75861.3330.30130.6251-0.2466-0.16640.9386-0.00180.761446.340414.783668.2463
193.05564.75612.60677.96074.17315.26570.2332-1.57110.81240.28850.2653-0.62050.64450.0201-0.46210.8964-0.11890.02561.0648-0.0720.835745.824228.293870.2719
200.25750.06170.3020.06230.19820.6862-0.04950.1627-0.4482-0.33630.3444-0.30750.614-0.2304-0.22381.229-0.4136-0.18490.75240.35471.546450.038329.432556.2437
210.38730.2445-0.17173.9011.0920.45750.0879-0.09260.32460.2274-0.07770.1411-1.15840.0251-0.18851.3779-0.09680.09280.1777-0.03330.338624.25524.872984.9676
223.29862.7257-4.63633.6877-4.69257.3908-0.1686-1.54861.30911.204-0.3429-2.5851-1.2952.08640.46250.909-0.1355-0.32110.90390.01710.978837.99238.47885.5394
230.7039-0.3814-0.02172.98450.69722.76360.1991-0.1134-0.01950.6227-0.12710.1163-0.1115-0.3872-0.04910.28390.00650.09990.160.02350.156819.3051.570580.7648
240.1530.1508-0.10571.2315-0.31011.15650.20390.15810.2295-0.0489-0.09160.0874-1.1226-0.2212-0.08720.54210.09030.02840.23950.05980.191719.705312.48365.4451
259.88513.76683.51268.70426.11164.38270.368-0.50771.0222-0.4364-0.56450.7302-1.0474-1.12610.38011.02160.25810.25580.42210.02190.45619.888420.176470.5345
260.5774-0.0038-0.18680.8339-0.76441.25990.09520.12040.19230.13760.00460.1005-0.9913-0.1629-0.09631.0984-0.03870.13120.00330.11980.221822.274414.817477.6688
272.0157-0.4049-0.2393.5310.9012.36190.1027-0.2123-0.25520.2366-0.1112-0.1940.40340.44010.02950.33820.07-0.05510.28150.080.200532.186-17.89777.913
285.2571.45482.40732.7765-0.25998.30340.04440.7788-0.0571-0.531-0.2165-0.98840.07011.09890.20250.6810.30770.05670.5740.06220.506144.8744-24.048166.5004
292.32990.05910.44338.6233-2.59761.7982-0.06830.1846-0.07440.5309-0.2887-1.250.13710.60320.34090.42430.0358-0.00230.33720.07630.264334.6839-11.12866.5689
301.26171.3014-0.87867.2458-3.0281.4422-0.07090.062-0.2099-1.4281-0.41-0.35470.980.35640.49230.58520.14390.04070.44140.01960.252235.6451-21.246460.9325
310.8690.01410.44131.52320.27972.3619-0.02220.0149-0.1076-0.0259-0.0903-0.08140.4690.1960.11610.27450.05740.01670.15690.02440.144425.111-11.924163.7329
320.9081.4092-0.61917.1236-1.45265.6207-0.17891.01820.6547-0.53130.0042-0.5311-1.00771.11560.14150.4768-0.05790.08270.50160.13440.288230.765413.300150.689
331.91530.1593-0.03131.9782-0.75182.4150.03980.03790.0766-0.05980.04370.53260.1741-0.951-0.06250.312-0.0713-0.05060.4583-0.01850.24286.5008-6.340757.3906
342.31550.6093-0.29622.2840.2061.91960.2689-0.0304-0.09610.04740.15010.65290.1462-0.8943-0.37280.1981-0.0351-0.00660.52540.00870.25754.723-7.511561.5101
351.82350.95680.37034.5122-1.23250.82450.03930.23750.2424-0.37470.15220.7127-0.421-0.9542-0.22530.54550.2798-0.05380.76690.05870.35071.64769.760253.7295
360.93590.256-0.87660.1351-0.17670.86230.08960.31470.0442-0.30680.19340.6228-0.1715-0.9783-0.21040.30570.0503-0.09640.90990.06350.48260.26661.168250.9571
375.58341.54013.94175.17150.33462.9057-0.2060.00920.10250.09330.05651.5688-0.5336-0.88690.21570.29560.0904-0.03340.72330.05460.7255-2.137121.287626.8632
386.1677-1.75111.19062.71441.73426.4302-0.28280.1593-0.1961-0.32570.05881.0332-0.2378-1.22940.19380.3368-0.0261-0.12230.53670.11190.52622.593114.159619.6826
397.2612-1.6963-0.90462.7797-0.16913.9311-0.81531.03731.39651.03690.2901-0.0192-0.1537-0.15380.48161.2033-0.20760.02680.5580.18211.03431.659129.309519.1577
400.2277-0.29590.41930.5255-0.55080.7725-0.3968-0.8487-0.02910.36680.53780.3057-0.3025-0.2955-0.11310.52030.21860.0210.8088-0.3711.513-3.043730.210232.1014
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1(chain A and resid 1:10)A1 - 10
2X-RAY DIFFRACTION2(chain A and resid 11:51)A11 - 51
3X-RAY DIFFRACTION3(chain A and resid 52:62)A52 - 62
4X-RAY DIFFRACTION4(chain A and resid 63:83)A63 - 83
5X-RAY DIFFRACTION5(chain A and resid 84:222)A84 - 222
6X-RAY DIFFRACTION6(chain A and resid 223:262)A223 - 262
7X-RAY DIFFRACTION7(chain A and resid 263:293)A263 - 293
8X-RAY DIFFRACTION8(chain A and resid 294:308)A294 - 308
9X-RAY DIFFRACTION9(chain A and resid 309:361)A309 - 361
10X-RAY DIFFRACTION10(chain A and resid 362:376)A362 - 376
11X-RAY DIFFRACTION11(chain A and resid 377:403)A377 - 403
12X-RAY DIFFRACTION12(chain A and resid 404:428)A404 - 428
13X-RAY DIFFRACTION13(chain A and resid 429:448)A429 - 448
14X-RAY DIFFRACTION14(chain A and resid 449:563)A449 - 563
15X-RAY DIFFRACTION15(chain A and resid 564:626)A564 - 626
16X-RAY DIFFRACTION16(chain A and resid 627:669)A627 - 669
17X-RAY DIFFRACTION17(chain A and resid 670:693)A670 - 693
18X-RAY DIFFRACTION18(chain A and resid 694:731)A694 - 731
19X-RAY DIFFRACTION19(chain A and resid 732:742)A732 - 742
20X-RAY DIFFRACTION20(chain A and resid 743:750)A743 - 750
21X-RAY DIFFRACTION21(chain B and resid 3:50)B3 - 50
22X-RAY DIFFRACTION22(chain B and resid 51:63)B51 - 63
23X-RAY DIFFRACTION23(chain B and resid 64:105)B64 - 105
24X-RAY DIFFRACTION24(chain B and resid 106:221)B106 - 221
25X-RAY DIFFRACTION25(chain B and resid 222:225)B222 - 225
26X-RAY DIFFRACTION26(chain B and resid 226:327)B226 - 327
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28X-RAY DIFFRACTION28(chain B and resid 385:400)B385 - 400
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31X-RAY DIFFRACTION31(chain B and resid 448:513)B448 - 513
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33X-RAY DIFFRACTION33(chain B and resid 524:579)B524 - 579
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35X-RAY DIFFRACTION35(chain B and resid 624:650)B624 - 650
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38X-RAY DIFFRACTION38(chain B and resid 706:732)B706 - 732
39X-RAY DIFFRACTION39(chain B and resid 733:743)B733 - 743
40X-RAY DIFFRACTION40(chain B and resid 744:750)B744 - 750

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