- PDB-7moi: Crystal Structure of Arabidopsis thaliana Plant and Fungi Atypica... -
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データベース: PDB / ID: 7moi
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Crystal Structure of Arabidopsis thaliana Plant and Fungi Atypical Dual Specificity Phosphatase 1(AtPFA-DSP1 ) Cys150Ser in complex with Phenyl Phosphate
温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: 0.4 M NaCl, 100 mM HEPES PH 7.2, 50 mM beta-mercaptoethanol at 298K (3 ul of 5.5 mg/ml protein plus 1 ul of well buffer in the crystallization drop). The formed crystal was soaked in 30% ...詳細: 0.4 M NaCl, 100 mM HEPES PH 7.2, 50 mM beta-mercaptoethanol at 298K (3 ul of 5.5 mg/ml protein plus 1 ul of well buffer in the crystallization drop). The formed crystal was soaked in 30% PEG400, 13mM MgCl2, 33mM NaF, 50 mM beta-mercaptoethanol, 66 mM HEPES, pH 7.2, and 10 mM phenyl phosphate for 4 days.