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Open data
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Basic information
Entry | Database: PDB / ID: 7mld | ||||||
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Title | PYL10 bound to the ABA pan-antagonist antabactin | ||||||
![]() | Abscisic acid receptor PYL10 | ||||||
![]() | PLANT PROTEIN/ANTAGONIST / PYR/PYL/RCAR / PYL10 / HORMONE RECEPTOR / PLANT PROTEIN / PLANT PROTEIN-ANTAGONIST complex | ||||||
Function / homology | ![]() regulation of protein serine/threonine phosphatase activity / abscisic acid binding / abscisic acid-activated signaling pathway / protein phosphatase inhibitor activity / signaling receptor activity / protein homodimerization activity / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Peterson, F.C. / Vaidya, A.S. / Volkman, B.F. / Cutler, S.R. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Click-to-lead design of a picomolar ABA receptor antagonist with potent activity in vivo. Authors: Vaidya, A.S. / Peterson, F.C. / Eckhardt, J. / Xing, Z. / Park, S.Y. / Dejonghe, W. / Takeuchi, J. / Pri-Tal, O. / Faria, J. / Elzinga, D. / Volkman, B.F. / Todoroki, Y. / Mosquna, A. / ...Authors: Vaidya, A.S. / Peterson, F.C. / Eckhardt, J. / Xing, Z. / Park, S.Y. / Dejonghe, W. / Takeuchi, J. / Pri-Tal, O. / Faria, J. / Elzinga, D. / Volkman, B.F. / Todoroki, Y. / Mosquna, A. / Okamoto, M. / Cutler, S.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 62.1 KB | Display | ![]() |
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PDB format | ![]() | 34.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 723.9 KB | Display | ![]() |
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Full document | ![]() | 725.1 KB | Display | |
Data in XML | ![]() | 10.8 KB | Display | |
Data in CIF | ![]() | 15.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7mlcC ![]() 6nwbS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 17747.307 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ZLG / |
#3: Chemical | ChemComp-CIT / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 46.11 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 200 mM tribasic ammonium citrate and 22% (w/v) PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Jul 1, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54178 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→50 Å / Num. obs: 14845 / % possible obs: 97 % / Redundancy: 7.1 % / Biso Wilson estimate: 19.37 Å2 / CC1/2: 1 / CC star: 1 / Rmerge(I) obs: 0.051 / Rpim(I) all: 0.02 / Rrim(I) all: 0.055 / Net I/σ(I): 25.3 |
Reflection shell | Resolution: 1.8→1.86 Å / Rmerge(I) obs: 0.373 / Mean I/σ(I) obs: 2.6 / Num. unique obs: 1429 / CC1/2: 0.912 / CC star: 0.977 / Rpim(I) all: 0.181 / Rrim(I) all: 0.416 / % possible all: 86.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6NWB Resolution: 1.8→42.45 Å / SU ML: 0.2245 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 19.9493 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.38 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→42.45 Å
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Refine LS restraints |
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LS refinement shell |
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