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Yorodumi- PDB-7ju6: Structure of RET protein tyrosine kinase in complex with selpercatinib -
+Open data
-Basic information
Entry | Database: PDB / ID: 7ju6 | ||||||
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Title | Structure of RET protein tyrosine kinase in complex with selpercatinib | ||||||
Components | Proto-oncogene tyrosine-protein kinase receptor Ret | ||||||
Keywords | TRANSFERASE/TRANSFERASE INHIBITOR / Transferase / oncogene / RET / Tyrisine Kinase / ATP binding / Thyroid cancer / Non small cell lung cancer / LOXO-292 / selpercatinib / Transferase-Transferase inhibitor complex | ||||||
Function / homology | Function and homology information glial cell-derived neurotrophic factor receptor signaling pathway / Peyer's patch morphogenesis / positive regulation of metanephric glomerulus development / posterior midgut development / ureter maturation / embryonic epithelial tube formation / lymphocyte migration into lymphoid organs / membrane protein proteolysis / positive regulation of peptidyl-serine phosphorylation of STAT protein / positive regulation of neuron maturation ...glial cell-derived neurotrophic factor receptor signaling pathway / Peyer's patch morphogenesis / positive regulation of metanephric glomerulus development / posterior midgut development / ureter maturation / embryonic epithelial tube formation / lymphocyte migration into lymphoid organs / membrane protein proteolysis / positive regulation of peptidyl-serine phosphorylation of STAT protein / positive regulation of neuron maturation / neuron cell-cell adhesion / enteric nervous system development / innervation / plasma membrane protein complex / neuron maturation / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / positive regulation of cell adhesion mediated by integrin / neural crest cell migration / ureteric bud development / response to pain / regulation of axonogenesis / homophilic cell adhesion via plasma membrane adhesion molecules / positive regulation of cell size / RET signaling / regulation of cell adhesion / cellular response to retinoic acid / NPAS4 regulates expression of target genes / transmembrane receptor protein tyrosine kinase activity / axon guidance / receptor protein-tyrosine kinase / positive regulation of neuron projection development / cell surface receptor protein tyrosine kinase signaling pathway / activation of cysteine-type endopeptidase activity involved in apoptotic process / MAPK cascade / retina development in camera-type eye / signaling receptor activity / RAF/MAP kinase cascade / protein tyrosine kinase activity / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / receptor complex / early endosome / endosome membrane / positive regulation of cell migration / response to xenobiotic stimulus / axon / protein phosphorylation / neuronal cell body / dendrite / calcium ion binding / positive regulation of gene expression / positive regulation of DNA-templated transcription / signal transduction / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.06 Å | ||||||
Authors | Terzyan, S.S. / Shen, T. / Wu, J. / Mooers, B.H.M. | ||||||
Funding support | United States, 1items
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Citation | Journal: Ann Oncol / Year: 2021 Title: Structural basis of acquired resistance to selpercatinib and pralsetinib mediated by non-gatekeeper RET mutations. Authors: Subbiah, V. / Shen, T. / Terzyan, S.S. / Liu, X. / Hu, X. / Patel, K.P. / Hu, M. / Cabanillas, M. / Behrang, A. / Meric-Bernstam, F. / Vo, P.T.T. / Mooers, B.H.M. / Wu, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7ju6.cif.gz | 137.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7ju6.ent.gz | 104.8 KB | Display | PDB format |
PDBx/mmJSON format | 7ju6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ju/7ju6 ftp://data.pdbj.org/pub/pdb/validation_reports/ju/7ju6 | HTTPS FTP |
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-Related structure data
Related structure data | 7ju5C 6necS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 35708.234 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RET, CDHF12, CDHR16, PTC, RET51 / Plasmid: PBACPAK6 / Cell line (production host): Sf21 Production host: Spodoptera aff. frugiperda 2 RZ-2014 (butterflies/moths) References: UniProt: P07949, receptor protein-tyrosine kinase #2: Chemical | #3: Chemical | ChemComp-FMT / #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 48.91 % / Description: transparent small crystals |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 5.5 / Details: 21-30% PEG3350 0.1M Na Citrate pH5.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL14-1 / Wavelength: 1 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 13, 2019 / Details: Rh coated collimator |
Radiation | Monochromator: Double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.05→100 Å / Num. obs: 37447 / % possible obs: 97.8 % / Observed criterion σ(I): -3 / Redundancy: 3.3 % / Biso Wilson estimate: 27.09 Å2 / Rmerge(I) obs: 0.12 / Rpim(I) all: 0.077 / Rrim(I) all: 0.143 / Χ2: 0.968 / Net I/σ(I): 9.7 |
Reflection shell | Resolution: 2.05→2.12 Å / Redundancy: 3 % / Rmerge(I) obs: 0.844 / Mean I/σ(I) obs: 1.5 / Num. unique obs: 3710 / CC1/2: 0.991 / Rpim(I) all: 0.578 / Rrim(I) all: 1.028 / Χ2: 0.925 / % possible all: 97 |
-Processing
Software |
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Refinement | Method to determine structure: FOURIER SYNTHESIS Starting model: 6NEC Resolution: 2.06→78.61 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.922 / SU B: 6.724 / SU ML: 0.17 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.239 / ESU R Free: 0.199 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 96.76 Å2 / Biso mean: 31.034 Å2 / Biso min: 13.77 Å2
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Refinement step | Cycle: final / Resolution: 2.06→78.61 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.064→2.118 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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