+Open data
-Basic information
Entry | Database: PDB / ID: 7jnn | ||||||
---|---|---|---|---|---|---|---|
Title | NMR Solution Structure of plant defensin SlD26 | ||||||
Components | SlD26 plant defensin | ||||||
Keywords | ANTIFUNGAL PROTEIN / plant defensin | ||||||
Function / homology | Knottin, scorpion toxin-like superfamily / Uncharacterized protein Function and homology information | ||||||
Biological species | Solanum lycopersicum (tomato) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Harvey, P.J. / Craik, D.J. | ||||||
Citation | Journal: J Fungi (Basel) / Year: 2020 Title: Histidine-Rich Defensins from the Solanaceae and Brasicaceae Are Antifungal and Metal Binding Proteins. Authors: Bleackley, M.R. / Vasa, S. / Harvey, P.J. / Shafee, T.M.A. / Kerenga, B.K. / Soares da Costa, T.P. / Craik, D.J. / Lowe, R.G.T. / Anderson, M.A. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 7jnn.cif.gz | 269.7 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb7jnn.ent.gz | 228.8 KB | Display | PDB format |
PDBx/mmJSON format | 7jnn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7jnn_validation.pdf.gz | 502.3 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 7jnn_full_validation.pdf.gz | 582.6 KB | Display | |
Data in XML | 7jnn_validation.xml.gz | 17.7 KB | Display | |
Data in CIF | 7jnn_validation.cif.gz | 28.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jn/7jnn ftp://data.pdbj.org/pub/pdb/validation_reports/jn/7jnn | HTTPS FTP |
-Related structure data
Related structure data | 7jn6C C: citing same article (ref.) |
---|---|
Similar structure data | |
Other databases |
|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein/peptide | Mass: 5444.244 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Solanum lycopersicum (tomato) / Production host: Komagataella pastoris (fungus) / References: UniProt: A0A3Q7H5E3 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| |||||||||||||||
Sample conditions | Ionic strength units: Not defined / Label: conditions_1 / pH: 4 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III HD / Manufacturer: Bruker / Model: AVANCE III HD / Field strength: 600 MHz |
---|
-Processing
NMR software |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 4 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 50 / Conformers submitted total number: 20 |