iron ion sequestering activity / : / autolysosome / Scavenging by Class A Receptors / Golgi Associated Vesicle Biogenesis / ferroxidase / intracellular sequestering of iron ion / ferroxidase activity / negative regulation of fibroblast proliferation / ferric iron binding ...iron ion sequestering activity / : / autolysosome / Scavenging by Class A Receptors / Golgi Associated Vesicle Biogenesis / ferroxidase / intracellular sequestering of iron ion / ferroxidase activity / negative regulation of fibroblast proliferation / ferric iron binding / Iron uptake and transport / ferrous iron binding / tertiary granule lumen / iron ion transport / intracellular iron ion homeostasis / ficolin-1-rich granule lumen / iron ion binding / immune response / negative regulation of cell population proliferation / Neutrophil degranulation / extracellular exosome / extracellular region / identical protein binding / membrane / nucleus / cytoplasm / cytosol 類似検索 - 分子機能
温度: 298 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 9.5 詳細: Reservoir: 500 uL total volume: 50 mM CHES (pH 9.5), 150 mM NaCl, 0.474 mM NiCl2, 12.6% PEP Sitting Drop: 12.7 uL reservoir, 3.3 uL of 25 uM ferritin, 4 uL of 10 mM fdh in 50 mM CHES (pH 9.5) with 150 mM NaCl
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データ収集
回折
平均測定温度: 278 K / Serial crystal experiment: N
放射光源
由来: 回転陽極 / タイプ: BRUKER AXS MICROSTAR / 波長: 1.54178 Å
検出器
タイプ: APEX II CCD / 検出器: CCD / 日付: 2019年9月6日
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.54178 Å / 相対比: 1
反射
解像度: 3.08→49.36 Å / Num. obs: 11398 / % possible obs: 100 % / 冗長度: 18.4 % / Biso Wilson estimate: 48.66 Å2 / CC1/2: 0.996 / Net I/σ(I): 9.8