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- PDB-7fi6: Crystal structure of human MICA mutants in complex with natural k... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7fi6 | ||||||
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Title | Crystal structure of human MICA mutants in complex with natural killer cell receptor NKG2D | ||||||
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![]() | IMMUNE SYSTEM / NKG2D / MICA / thermal stability | ||||||
Function / homology | ![]() immune response to tumor cell / negative regulation of natural killer cell chemotaxis / MHC class Ib receptor activity / natural killer cell lectin-like receptor binding / negative regulation of natural killer cell activation / negative regulation of natural killer cell mediated cytotoxicity / gamma-delta T cell activation / positive regulation of natural killer cell mediated cytotoxicity / natural killer cell mediated cytotoxicity / natural killer cell activation ...immune response to tumor cell / negative regulation of natural killer cell chemotaxis / MHC class Ib receptor activity / natural killer cell lectin-like receptor binding / negative regulation of natural killer cell activation / negative regulation of natural killer cell mediated cytotoxicity / gamma-delta T cell activation / positive regulation of natural killer cell mediated cytotoxicity / natural killer cell mediated cytotoxicity / natural killer cell activation / negative regulation of GTPase activity / MHC class I protein binding / stimulatory C-type lectin receptor signaling pathway / T cell costimulation / nitric oxide biosynthetic process / DAP12 interactions / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / T cell mediated cytotoxicity / positive regulation of T cell mediated cytotoxicity / peptide antigen binding / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / positive regulation of nitric oxide biosynthetic process / positive regulation of type II interferon production / DAP12 signaling / signaling receptor activity / response to heat / carbohydrate binding / cellular response to lipopolysaccharide / defense response to virus / adaptive immune response / killing of cells of another organism / cell differentiation / defense response to Gram-positive bacterium / defense response to bacterium / immune response / external side of plasma membrane / signaling receptor binding / DNA damage response / cell surface / signal transduction / extracellular space / identical protein binding / membrane / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cai, W. / Peng, S. / Xu, T. / Tian, Y. / Li, Y. / Liu, J. | ||||||
![]() | ![]() Title: Crystal structure of human MICA mutants in complex with natural killer cell receptor NKG2D Authors: Cai, W. / Peng, S. / Xu, T. / Tian, Y. / Li, Y. / Liu, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 117.9 KB | Display | ![]() |
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PDB format | ![]() | 90 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.2 KB | Display | ![]() |
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Full document | ![]() | 456.6 KB | Display | |
Data in XML | ![]() | 19.9 KB | Display | |
Data in CIF | ![]() | 26.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7fi5C ![]() 7fi7C ![]() 7fi8C ![]() 7fi9C ![]() 1hyrS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Ens-ID: 1 / Beg auth comp-ID: GLU / Beg label comp-ID: GLU / End auth comp-ID: ARG / End label comp-ID: ARG / Auth seq-ID: 93 - 214 / Label seq-ID: 16 - 137
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Components
#1: Protein | Mass: 16110.215 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | | Mass: 31764.508 Da / Num. of mol.: 1 / Mutation: H161R, V177I Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 54.4 % / Mosaicity: 0.54 ° |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / pH: 7 / Details: 2.7 M Sodium chloride, 0.1 M Tris pH 8.7 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 24, 2020 | ||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97915 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 2.9→90.38 Å / Num. obs: 15918 / % possible obs: 100 % / Redundancy: 24 % / CC1/2: 0.995 / Rmerge(I) obs: 0.36 / Rpim(I) all: 0.074 / Rrim(I) all: 0.368 / Net I/σ(I): 12 / Num. measured all: 382274 / Scaling rejects: 868 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1hyr Resolution: 2.9→62.91 Å / Cor.coef. Fo:Fc: 0.932 / Cor.coef. Fo:Fc free: 0.881 / SU B: 16.784 / SU ML: 0.306 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.406 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 146.79 Å2 / Biso mean: 53.313 Å2 / Biso min: 24.4 Å2
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Refinement step | Cycle: final / Resolution: 2.9→62.91 Å
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Refine LS restraints |
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Refine LS restraints NCS | Ens-ID: 1 / Number: 3558 / Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Rms dev position: 0.16 Å / Weight position: 0.05
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LS refinement shell | Resolution: 2.9→2.976 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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