+Open data
-Basic information
Entry | Database: PDB / ID: 7f73 | ||||||
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Title | Cryo-EM structure of human TMEM120B | ||||||
Components | MCherry fluorescent protein,Transmembrane protein 120B | ||||||
Keywords | MEMBRANE PROTEIN / Membran protein | ||||||
Function / homology | Function and homology information protein heterooligomerization / nuclear inner membrane / fat cell differentiation / bioluminescence / generation of precursor metabolites and energy / monoatomic ion transmembrane transport / membrane => GO:0016020 / membrane Similarity search - Function | ||||||
Biological species | Anaplasma marginale (bacteria) Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4 Å | ||||||
Authors | Ke, M. / Wu, J. / Yan, Z. | ||||||
Citation | Journal: Cell Discov / Year: 2021 Title: Cryo-EM structures of human TMEM120A and TMEM120B. Authors: Meng Ke / Yue Yu / Changjian Zhao / Shirong Lai / Qiang Su / Weidan Yuan / Lina Yang / Dong Deng / Kun Wu / Weizheng Zeng / Jia Geng / Jianping Wu / Zhen Yan / | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7f73.cif.gz | 127.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7f73.ent.gz | 93.2 KB | Display | PDB format |
PDBx/mmJSON format | 7f73.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7f73_validation.pdf.gz | 1 MB | Display | wwPDB validaton report |
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Full document | 7f73_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 7f73_validation.xml.gz | 28.1 KB | Display | |
Data in CIF | 7f73_validation.cif.gz | 37.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f7/7f73 ftp://data.pdbj.org/pub/pdb/validation_reports/f7/7f73 | HTTPS FTP |
-Related structure data
Related structure data | 31484MC 7cxrC C: citing same article (ref.) M: map data used to model this data |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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-Components
#1: Protein | Mass: 72284.109 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Anaplasma marginale (bacteria), (gene. exp.) Homo sapiens (human) Gene: mCherry, TMEM120B / Production host: Homo sapiens (human) / References: UniProt: X5DSL3, UniProt: A0PK00 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Dimeric human TMEM120B / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT | ||||||||||||
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Source (natural) |
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Source (recombinant) | Organism: Homo sapiens (human) | ||||||||||||
Buffer solution | pH: 8 | ||||||||||||
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.18.1_3865: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: NONE | ||||||||||||||||||||||||
3D reconstruction | Resolution: 4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 137998 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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