+Open data
-Basic information
Entry | Database: PDB / ID: 7f45 | ||||||
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Title | Structure of an Anti-CRISPR protein | ||||||
Components | AcrIF5 | ||||||
Keywords | IMMUNE SYSTEM / monomer / mixed alpha-beta structure | ||||||
Function / homology | : Function and homology information | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.52 Å | ||||||
Authors | Feng, Y. | ||||||
Funding support | China, 1items
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Citation | Journal: Nat Chem Biol / Year: 2022 Title: AcrIF5 specifically targets DNA-bound CRISPR-Cas surveillance complex for inhibition. Authors: Yongchao Xie / Laixing Zhang / Zhengyu Gao / Peipei Yin / Hao Wang / Hang Li / Zeliang Chen / Yi Zhang / Maojun Yang / Yue Feng / Abstract: CRISPR-Cas systems are prokaryotic antiviral systems, and phages use anti-CRISPR proteins (Acrs) to inactivate these systems. Here we present structural and functional analyses of AcrIF5, exploring ...CRISPR-Cas systems are prokaryotic antiviral systems, and phages use anti-CRISPR proteins (Acrs) to inactivate these systems. Here we present structural and functional analyses of AcrIF5, exploring its unique anti-CRISPR mechanism. AcrIF5 shows binding specificity only for the target DNA-bound form of the crRNA-guided surveillance (Csy) complex, but not the apo Csy complex from the type I-F CRISPR-Cas system. We solved the structure of the Csy-dsDNA-AcrIF5 complex, revealing that the conformational changes of the Csy complex caused by dsDNA binding dictate the binding specificity for the Csy-dsDNA complex by AcrIF5. Mechanistically, five AcrIF5 molecules bind one Csy-dsDNA complex, which destabilizes the helical bundle domain of Cas8f, thus preventing subsequent Cas2/3 recruitment. AcrIF5 exists in symbiosis with AcrIF3, which blocks Cas2/3 recruitment. This attack on the recruitment event stands in contrast to the conventional mechanisms of blocking binding of target DNA. Overall, our study reveals an unprecedented mechanism of CRISPR-Cas inhibition by AcrIF5. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7f45.cif.gz | 70.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7f45.ent.gz | 51.5 KB | Display | PDB format |
PDBx/mmJSON format | 7f45.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7f45_validation.pdf.gz | 432.7 KB | Display | wwPDB validaton report |
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Full document | 7f45_full_validation.pdf.gz | 434.9 KB | Display | |
Data in XML | 7f45_validation.xml.gz | 7.8 KB | Display | |
Data in CIF | 7f45_validation.cif.gz | 9.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f4/7f45 ftp://data.pdbj.org/pub/pdb/validation_reports/f4/7f45 | HTTPS FTP |
-Related structure data
Related structure data | 7eqgSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Ens-ID: 1
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