+Open data
-Basic information
Entry | Database: PDB / ID: 7ezb | ||||||||||||
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Title | Thorarchaeota Rab bound to GDP | ||||||||||||
Components | Thorarchaeota Rab | ||||||||||||
Keywords | SIGNALING PROTEIN / Rab / Small GTPase | ||||||||||||
Function / homology | Function and homology information | ||||||||||||
Biological species | Candidatus Thorarchaeota archaeon SMTZ1-45 (archaea) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.5 Å | ||||||||||||
Authors | Robinson, R.C. / Tran, L.T. | ||||||||||||
Funding support | Japan, United States, 3items
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Citation | Journal: To Be Published Title: Structure of Thor-Rab Authors: Robinson, R.C. / Tran, L.T. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7ezb.cif.gz | 168.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7ezb.ent.gz | 132.5 KB | Display | PDB format |
PDBx/mmJSON format | 7ezb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7ezb_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
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Full document | 7ezb_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 7ezb_validation.xml.gz | 19.6 KB | Display | |
Data in CIF | 7ezb_validation.cif.gz | 29.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ez/7ezb ftp://data.pdbj.org/pub/pdb/validation_reports/ez/7ezb | HTTPS FTP |
-Related structure data
Related structure data | 7ezdC 7ezeC 3bfkS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 20887.570 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Candidatus Thorarchaeota archaeon SMTZ1-45 (archaea) Gene: AM325_13980 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A135VL07 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43.31 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion Details: 100 mM MES, pH 5.5, 250 mM NaCl, 13% PEG 8000, 5 mM GTP |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: NSRRC / Beamline: TPS 05A / Wavelength: 1 Å |
Detector | Type: RAYONIX MX-300 / Detector: CCD / Date: May 28, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→20 Å / Num. obs: 56552 / % possible obs: 100 % / Redundancy: 5.4 % / Rmerge(I) obs: 0.103 / Net I/σ(I): 11.4 |
Reflection shell | Resolution: 1.5→1.53 Å / Redundancy: 3.8 % / Rmerge(I) obs: 1.012 / Mean I/σ(I) obs: 1.9 / Num. unique obs: 4850 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3BFK Resolution: 1.5→17.2 Å / Cross valid method: FREE R-VALUE Stereochemistry target values: GEOSTD + MONOMER LIBRARY + CDL V1.2
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Displacement parameters | Biso mean: 23.62 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→17.2 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.5→1.554 Å
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