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- PDB-7eyw: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with terre... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7eyw | ||||||
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Title | Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with terretonin C | ||||||
![]() | 2-oxoglutarate/Fe(II)-dependent dioxygenase SptF | ||||||
![]() | OXIDOREDUCTASE / alpha-ketoglutarate dependent dioxygenase / meroterpenoid | ||||||
Function / homology | Phytanoyl-CoA dioxygenase / Phytanoyl-CoA dioxygenase (PhyH) / dioxygenase activity / metal ion binding / Terretonin C / : / N-OXALYLGLYCINE / 2-oxoglutarate/Fe(II)-dependent dioxygenase SptF![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tao, H. / Mori, T. / Abe, I. | ||||||
Funding support | 1items
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![]() | ![]() Title: Molecular insights into the unusually promiscuous and catalytically versatile Fe(II)/ alpha-ketoglutarate-dependent oxygenase SptF. Authors: Tao, H. / Mori, T. / Chen, H. / Lyu, S. / Nonoyama, A. / Lee, S. / Abe, I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 235 KB | Display | ![]() |
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PDB format | ![]() | 182.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 830.1 KB | Display | ![]() |
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Full document | ![]() | 837.6 KB | Display | |
Data in XML | ![]() | 42 KB | Display | |
Data in CIF | ![]() | 58.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7eyrSC ![]() 7eysC ![]() 7eytC ![]() 7eyuC ![]() 7fcbC S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 33827.184 Da / Num. of mol.: 4 / Mutation: S114A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-OGA / #3: Chemical | ChemComp-FE2 / #4: Chemical | ChemComp-0IP / | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.94 Å3/Da / Density % sol: 36.54 % |
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Crystal grow | Temperature: 283 K / Method: vapor diffusion, sitting drop Details: 0.27 M magnesium chloride, 20% w/v PEG 3350, 0.2 M sodium chloride, 1.5% trimethylamine N-oxide dihydrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 4M / Detector: PIXEL / Date: Mar 27, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.07 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→48.24 Å / Num. obs: 57878 / % possible obs: 97.6 % / Redundancy: 2.1 % / Biso Wilson estimate: 28.4 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.065 / Net I/σ(I): 7.3 |
Reflection shell | Resolution: 2.1→2.16 Å / Redundancy: 2.1 % / Rmerge(I) obs: 0.632 / Mean I/σ(I) obs: 1.8 / Num. unique obs: 9566 / CC1/2: 0.871 / % possible all: 96.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 7EYR Resolution: 2.1→48.24 Å / SU ML: 0.2901 / Cross valid method: FREE R-VALUE / σ(F): 2.01 / Phase error: 29.1052 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.97 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→48.24 Å
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Refine LS restraints |
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LS refinement shell |
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