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Open data
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Basic information
Entry | Database: PDB / ID: 7eyp | ||||||
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Title | Crystal structure of Pseudomonas aeruginosa ppnP | ||||||
![]() | Pyrimidine/purine nucleoside phosphorylase | ||||||
![]() | HYDROLASE / Pyrimidine / purine / nucleoside phosphorylase | ||||||
Function / homology | ![]() guanosine phosphorylase / pyrimidine-nucleoside phosphorylase / thymidine phosphorylase / pyrimidine-nucleoside phosphorylase activity / thymidine phosphorylase activity / uridine phosphorylase / guanosine phosphorylase activity / uridine phosphorylase activity / purine-nucleoside phosphorylase / purine-nucleoside phosphorylase activity Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wen, Y. / Wu, B.X. | ||||||
![]() | ![]() Title: Crystal structures of a new class of pyrimidine/purine nucleoside phosphorylase revealed a Cupin fold. Authors: Wen, Y. / Li, X. / Guo, W. / Wu, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 59.3 KB | Display | ![]() |
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PDB format | ![]() | 41 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 428.6 KB | Display | ![]() |
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Full document | ![]() | 429.4 KB | Display | |
Data in XML | ![]() | 12.8 KB | Display | |
Data in CIF | ![]() | 18.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7eyjSC ![]() 7eykC ![]() 7eylC ![]() 7eymC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10250.493 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52.28 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.1 M BIS-TRIS pH 5.5, 2.0 M Ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 27, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97915 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→30 Å / Num. obs: 33692 / % possible obs: 97.6 % / Redundancy: 19.1 % / CC1/2: 0.989 / CC star: 0.997 / Rmerge(I) obs: 0.103 / Rpim(I) all: 0.024 / Rrim(I) all: 0.105 / Net I/σ(I): 30.875 |
Reflection shell | Resolution: 1.5→1.55 Å / Redundancy: 19.1 % / Rmerge(I) obs: 0.74 / Mean I/σ(I) obs: 6.75 / Num. unique obs: 3228 / CC1/2: 0.932 / CC star: 0.982 / Rpim(I) all: 0.171 / Rrim(I) all: 0.76 / % possible all: 96.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 7EYJ Resolution: 1.5→20 Å / Cross valid method: FREE R-VALUE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Displacement parameters | Biso mean: 17.32 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→20 Å
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Refine LS restraints |
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