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- PDB-7eyd: Cryo-EM structure of cyanobacterial phycobilisome from Anabaena s... -

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Basic information

Entry
Database: PDB / ID: 7eyd
TitleCryo-EM structure of cyanobacterial phycobilisome from Anabaena sp. PCC 7120
Components
  • (Allophycocyanin subunit ...) x 4
  • (C-phycocyanin ...) x 2
  • (Phycobilisome ...) x 5
  • Phycobiliprotein ApcE
KeywordsPHOTOSYNTHESIS
Function / homology
Function and homology information


Lyases / : / phycobilisome / plasma membrane-derived thylakoid membrane / photosynthesis / lyase activity
Similarity search - Function
Allophycocyanin linker protein / Allophycocyanin linker chain / Phycobilisome linker protein / Allophycocyanin, beta subunit / Phycobilisome linker domain / Phycobilisome linker domain superfamily / Phycobilisome Linker polypeptide / Phycobilisome (PBS) linker domain profile. / Phycocyanin, alpha subunit / Phycocyanin, beta subunit ...Allophycocyanin linker protein / Allophycocyanin linker chain / Phycobilisome linker protein / Allophycocyanin, beta subunit / Phycobilisome linker domain / Phycobilisome linker domain superfamily / Phycobilisome Linker polypeptide / Phycobilisome (PBS) linker domain profile. / Phycocyanin, alpha subunit / Phycocyanin, beta subunit / CpcD-like domain / CpcD/allophycocyanin linker domain / CpcD-like domain profile. / CpcD/allophycocyanin linker domain / Phycobilisome, alpha/beta subunit / Phycobilisome, alpha/beta subunit superfamily / Phycobilisome protein / Globin-like superfamily
Similarity search - Domain/homology
PHYCOCYANOBILIN / C-phycocyanin beta subunit / C-phycocyanin alpha subunit / Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod / Phycobilisome rod-core linker polypeptide CpcG1 / Phycobilisome rod-core linker polypeptide CpcG2 / Phycobilisome rod-core linker polypeptide CpcG4 / Allophycocyanin subunit alpha 1 / Allophycocyanin subunit alpha-B / Allophycocyanin subunit beta ...PHYCOCYANOBILIN / C-phycocyanin beta subunit / C-phycocyanin alpha subunit / Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod / Phycobilisome rod-core linker polypeptide CpcG1 / Phycobilisome rod-core linker polypeptide CpcG2 / Phycobilisome rod-core linker polypeptide CpcG4 / Allophycocyanin subunit alpha 1 / Allophycocyanin subunit alpha-B / Allophycocyanin subunit beta / Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core / Phycobiliprotein ApcE / Allophycocyanin subunit beta-18
Similarity search - Component
Biological speciesNostoc sp. PCC 7120 = FACHB-418 (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsZheng, L. / Zheng, Z. / Li, X. / Wang, G. / Zhang, K. / Wei, P. / Zhao, J. / Gao, N.
Funding support1items
OrganizationGrant numberCountry
National Science Foundation (NSF, China)
CitationJournal: Nat Commun / Year: 2021
Title: Structural insight into the mechanism of energy transfer in cyanobacterial phycobilisomes.
Authors: Lvqin Zheng / Zhenggao Zheng / Xiying Li / Guopeng Wang / Kun Zhang / Peijun Wei / Jindong Zhao / Ning Gao /
Abstract: Phycobilisomes (PBS) are the major light-harvesting machineries for photosynthesis in cyanobacteria and red algae and they have a hierarchical structure of a core and peripheral rods, with both ...Phycobilisomes (PBS) are the major light-harvesting machineries for photosynthesis in cyanobacteria and red algae and they have a hierarchical structure of a core and peripheral rods, with both consisting of phycobiliproteins and linker proteins. Here we report the cryo-EM structures of PBS from two cyanobacterial species, Anabaena 7120 and Synechococcus 7002. Both PBS are hemidiscoidal in shape and share a common triangular core structure. While the Anabaena PBS has two additional hexamers in the core linked by the 4th linker domain of ApcE (L). The PBS structures predict that, compared with the PBS from red algae, the cyanobacterial PBS could have more direct routes for energy transfer to ApcD. Structure-based systematic mutagenesis analysis of the chromophore environment of ApcD and ApcF subunits reveals that aromatic residues are critical to excitation energy transfer (EET). The structures also suggest that the linker protein could actively participate in the process of EET in both rods and the cores. These results provide insights into the organization of chromophores and the mechanisms of EET within cyanobacterial PBS.
History
DepositionMay 30, 2021Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Oct 6, 2021Provider: repository / Type: Initial release

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Assembly

Deposited unit
A1: Phycobilisome rod-core linker polypeptide CpcG2
B1: C-phycocyanin alpha subunit
C1: C-phycocyanin beta subunit
D1: C-phycocyanin alpha subunit
E1: C-phycocyanin beta subunit
F1: C-phycocyanin alpha subunit
G1: C-phycocyanin beta subunit
H1: C-phycocyanin alpha subunit
I1: C-phycocyanin beta subunit
J1: C-phycocyanin alpha subunit
K1: C-phycocyanin beta subunit
L1: C-phycocyanin alpha subunit
M1: C-phycocyanin beta subunit
N1: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
O1: C-phycocyanin alpha subunit
P1: C-phycocyanin beta subunit
Q1: C-phycocyanin alpha subunit
R1: C-phycocyanin beta subunit
S1: C-phycocyanin alpha subunit
T1: C-phycocyanin beta subunit
U1: C-phycocyanin alpha subunit
V1: C-phycocyanin beta subunit
W1: C-phycocyanin alpha subunit
X1: C-phycocyanin beta subunit
Y1: C-phycocyanin alpha subunit
Z1: C-phycocyanin beta subunit
A2: Phycobilisome rod-core linker polypeptide CpcG4
B2: C-phycocyanin alpha subunit
C2: C-phycocyanin beta subunit
D2: C-phycocyanin alpha subunit
E2: C-phycocyanin beta subunit
F2: C-phycocyanin alpha subunit
G2: C-phycocyanin beta subunit
H2: C-phycocyanin alpha subunit
I2: C-phycocyanin beta subunit
J2: C-phycocyanin alpha subunit
K2: C-phycocyanin beta subunit
L2: C-phycocyanin alpha subunit
M2: C-phycocyanin beta subunit
N2: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
O2: C-phycocyanin alpha subunit
P2: C-phycocyanin beta subunit
Q2: C-phycocyanin alpha subunit
R2: C-phycocyanin beta subunit
S2: C-phycocyanin alpha subunit
T2: C-phycocyanin beta subunit
U2: C-phycocyanin alpha subunit
V2: C-phycocyanin beta subunit
W2: C-phycocyanin alpha subunit
X2: C-phycocyanin beta subunit
Y2: C-phycocyanin alpha subunit
Z2: C-phycocyanin beta subunit
A3: Phycobilisome rod-core linker polypeptide CpcG1
B3: C-phycocyanin alpha subunit
C3: C-phycocyanin beta subunit
D3: C-phycocyanin alpha subunit
E3: C-phycocyanin beta subunit
F3: C-phycocyanin alpha subunit
G3: C-phycocyanin beta subunit
H3: C-phycocyanin alpha subunit
I3: C-phycocyanin beta subunit
J3: C-phycocyanin alpha subunit
K3: C-phycocyanin beta subunit
L3: C-phycocyanin alpha subunit
M3: C-phycocyanin beta subunit
N3: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
A4: Phycobilisome rod-core linker polypeptide CpcG2
B4: C-phycocyanin alpha subunit
C4: C-phycocyanin beta subunit
D4: C-phycocyanin alpha subunit
E4: C-phycocyanin beta subunit
F4: C-phycocyanin alpha subunit
G4: C-phycocyanin beta subunit
H4: C-phycocyanin alpha subunit
I4: C-phycocyanin beta subunit
J4: C-phycocyanin alpha subunit
K4: C-phycocyanin beta subunit
L4: C-phycocyanin alpha subunit
M4: C-phycocyanin beta subunit
N4: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
O4: C-phycocyanin alpha subunit
P4: C-phycocyanin beta subunit
Q4: C-phycocyanin alpha subunit
R4: C-phycocyanin beta subunit
S4: C-phycocyanin alpha subunit
T4: C-phycocyanin beta subunit
U4: C-phycocyanin alpha subunit
V4: C-phycocyanin beta subunit
W4: C-phycocyanin alpha subunit
X4: C-phycocyanin beta subunit
Y4: C-phycocyanin alpha subunit
Z4: C-phycocyanin beta subunit
A5: Phycobilisome rod-core linker polypeptide CpcG4
B5: C-phycocyanin alpha subunit
C5: C-phycocyanin beta subunit
D5: C-phycocyanin alpha subunit
E5: C-phycocyanin beta subunit
F5: C-phycocyanin alpha subunit
G5: C-phycocyanin beta subunit
H5: C-phycocyanin alpha subunit
I5: C-phycocyanin beta subunit
J5: C-phycocyanin alpha subunit
K5: C-phycocyanin beta subunit
L5: C-phycocyanin alpha subunit
M5: C-phycocyanin beta subunit
N5: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
O5: C-phycocyanin alpha subunit
P5: C-phycocyanin beta subunit
Q5: C-phycocyanin alpha subunit
R5: C-phycocyanin beta subunit
S5: C-phycocyanin alpha subunit
T5: C-phycocyanin beta subunit
U5: C-phycocyanin alpha subunit
V5: C-phycocyanin beta subunit
W5: C-phycocyanin alpha subunit
X5: C-phycocyanin beta subunit
Y5: C-phycocyanin alpha subunit
Z5: C-phycocyanin beta subunit
A6: Phycobilisome rod-core linker polypeptide CpcG4
B6: C-phycocyanin alpha subunit
C6: C-phycocyanin beta subunit
D6: C-phycocyanin alpha subunit
E6: C-phycocyanin beta subunit
F6: C-phycocyanin alpha subunit
G6: C-phycocyanin beta subunit
H6: C-phycocyanin alpha subunit
I6: C-phycocyanin beta subunit
J6: C-phycocyanin alpha subunit
K6: C-phycocyanin beta subunit
L6: C-phycocyanin alpha subunit
M6: C-phycocyanin beta subunit
N6: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
O6: C-phycocyanin alpha subunit
P6: C-phycocyanin beta subunit
Q6: C-phycocyanin alpha subunit
R6: C-phycocyanin beta subunit
S6: C-phycocyanin alpha subunit
T6: C-phycocyanin beta subunit
U6: C-phycocyanin alpha subunit
V6: C-phycocyanin beta subunit
W6: C-phycocyanin alpha subunit
X6: C-phycocyanin beta subunit
Y6: C-phycocyanin alpha subunit
Z6: C-phycocyanin beta subunit
A7: Phycobilisome rod-core linker polypeptide CpcG1
B7: C-phycocyanin alpha subunit
C7: C-phycocyanin beta subunit
D7: C-phycocyanin alpha subunit
E7: C-phycocyanin beta subunit
F7: C-phycocyanin alpha subunit
G7: C-phycocyanin beta subunit
H7: C-phycocyanin alpha subunit
I7: C-phycocyanin beta subunit
J7: C-phycocyanin alpha subunit
K7: C-phycocyanin beta subunit
L7: C-phycocyanin alpha subunit
M7: C-phycocyanin beta subunit
N7: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
A8: Allophycocyanin subunit alpha 1
B8: Allophycocyanin subunit beta
C8: Allophycocyanin subunit alpha 1
D8: Allophycocyanin subunit beta
E8: Allophycocyanin subunit alpha 1
F8: Allophycocyanin subunit beta
G8: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
H8: Allophycocyanin subunit alpha 1
I8: Allophycocyanin subunit beta
J8: Allophycocyanin subunit alpha 1
K8: Allophycocyanin subunit beta
L8: Allophycocyanin subunit alpha 1
M8: Allophycocyanin subunit beta
N8: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
O8: Allophycocyanin subunit alpha 1
P8: Allophycocyanin subunit beta
Q8: Allophycocyanin subunit alpha 1
R8: Allophycocyanin subunit beta
S8: Allophycocyanin subunit alpha 1
T8: Allophycocyanin subunit beta
U8: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
V8: Allophycocyanin subunit alpha 1
W8: Allophycocyanin subunit beta
X8: Allophycocyanin subunit alpha 1
Y8: Allophycocyanin subunit beta
Z8: Allophycocyanin subunit alpha 1
a8: Allophycocyanin subunit beta
b8: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
c8: Allophycocyanin subunit alpha 1
d8: Allophycocyanin subunit beta
e8: Allophycocyanin subunit alpha 1
f8: Allophycocyanin subunit beta
g8: Allophycocyanin subunit alpha 1
h8: Allophycocyanin subunit beta
i8: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
j8: Allophycocyanin subunit alpha 1
k8: Allophycocyanin subunit beta
l8: Allophycocyanin subunit alpha 1
m8: Allophycocyanin subunit beta
n8: Allophycocyanin subunit alpha 1
o8: Allophycocyanin subunit beta
p8: Allophycocyanin subunit alpha 1
q8: Allophycocyanin subunit beta
r8: Allophycocyanin subunit alpha 1
s8: Allophycocyanin subunit beta
t8: Allophycocyanin subunit alpha 1
u8: Allophycocyanin subunit beta
09: Phycobiliprotein ApcE
19: Phycobiliprotein ApcE
29: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
39: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
49: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
A9: Allophycocyanin subunit alpha 1
B9: Allophycocyanin subunit beta
C9: Allophycocyanin subunit alpha 1
D9: Allophycocyanin subunit beta
E9: Allophycocyanin subunit alpha 1
F9: Allophycocyanin subunit beta
G9: Allophycocyanin subunit alpha 1
H9: Allophycocyanin subunit beta
I9: Allophycocyanin subunit alpha 1
J9: Allophycocyanin subunit beta
K9: Allophycocyanin subunit alpha 1
L9: Allophycocyanin subunit beta
M9: Allophycocyanin subunit beta
N9: Allophycocyanin subunit alpha 1
O9: Allophycocyanin subunit beta
P9: Allophycocyanin subunit alpha 1
Q9: Allophycocyanin subunit beta-18
R9: Allophycocyanin subunit alpha 1
S9: Allophycocyanin subunit beta
T9: Allophycocyanin subunit alpha 1
U9: Allophycocyanin subunit beta
V9: Allophycocyanin subunit alpha-B
W9: Allophycocyanin subunit beta
X9: Allophycocyanin subunit alpha 1
Y9: Allophycocyanin subunit beta
Z9: Allophycocyanin subunit alpha 1
a9: Allophycocyanin subunit beta
b9: Allophycocyanin subunit alpha 1
c9: Allophycocyanin subunit beta
d9: Allophycocyanin subunit alpha 1
e9: Allophycocyanin subunit beta
f9: Allophycocyanin subunit alpha 1
g9: Allophycocyanin subunit beta-18
h9: Allophycocyanin subunit beta
i9: Allophycocyanin subunit alpha 1
j9: Allophycocyanin subunit beta
k9: Allophycocyanin subunit alpha 1
l9: Allophycocyanin subunit beta
m9: Allophycocyanin subunit alpha-B
n9: Allophycocyanin subunit beta
o9: Allophycocyanin subunit alpha 1
p9: Allophycocyanin subunit beta
q9: Allophycocyanin subunit alpha 1
r9: Allophycocyanin subunit beta
s9: Allophycocyanin subunit alpha 1
t9: Allophycocyanin subunit beta
u9: Allophycocyanin subunit alpha 1
v9: Allophycocyanin subunit beta
w9: Allophycocyanin subunit alpha 1
x9: Allophycocyanin subunit beta
y9: Allophycocyanin subunit alpha 1
z9: Allophycocyanin subunit beta
AA: Phycobilisome rod-core linker polypeptide CpcG4
BA: C-phycocyanin alpha subunit
CA: C-phycocyanin beta subunit
DA: C-phycocyanin alpha subunit
EA: C-phycocyanin beta subunit
FA: C-phycocyanin alpha subunit
GA: C-phycocyanin beta subunit
HA: C-phycocyanin alpha subunit
IA: C-phycocyanin beta subunit
JA: C-phycocyanin alpha subunit
KA: C-phycocyanin beta subunit
LA: C-phycocyanin alpha subunit
MA: C-phycocyanin beta subunit
NA: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
OA: C-phycocyanin alpha subunit
PA: C-phycocyanin beta subunit
QA: C-phycocyanin alpha subunit
RA: C-phycocyanin beta subunit
SA: C-phycocyanin alpha subunit
TA: C-phycocyanin beta subunit
UA: C-phycocyanin alpha subunit
VA: C-phycocyanin beta subunit
WA: C-phycocyanin alpha subunit
XA: C-phycocyanin beta subunit
YA: C-phycocyanin alpha subunit
ZA: C-phycocyanin beta subunit
hetero molecules


Theoretical massNumber of molelcules
Total (without water)5,666,114636
Polymers5,461,249288
Non-polymers204,866348
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: microscopy
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Phycobilisome ... , 5 types, 24 molecules A1A4N1N2N3N4N5N6N7NAA2A5A6AAA3A7G8N8U8b8i8293949

#1: Protein Phycobilisome rod-core linker polypeptide CpcG2 / L-RC 28.5


Mass: 28688.434 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Strain: PCC 7120 / Gene: cpcG2 / Production host: Nostoc sp. PCC 7120 = FACHB-418 (bacteria) / References: UniProt: P29987
#4: Protein
Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod


Mass: 32254.086 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Strain: PCC 7120 / Gene: cpcC, alr0530 / Production host: Nostoc sp. PCC 7120 = FACHB-418 (bacteria) / References: UniProt: P07123
#5: Protein
Phycobilisome rod-core linker polypeptide CpcG4 / L-RC 29.2


Mass: 29362.826 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Strain: PCC 7120 / Gene: cpcG4 / Production host: Nostoc sp. PCC 7120 = FACHB-418 (bacteria) / References: UniProt: P29989
#6: Protein Phycobilisome rod-core linker polypeptide CpcG1 / L-RC 31.8


Mass: 31973.932 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Strain: PCC 7120 / Gene: cpcG1 / Production host: Nostoc sp. PCC 7120 = FACHB-418 (bacteria) / References: UniProt: P29986
#9: Protein
Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core / LC 7.8


Mass: 7852.082 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Strain: PCC 7120 / Gene: apcC / Production host: Nostoc sp. PCC 7120 = FACHB-418 (bacteria) / References: UniProt: P80558

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C-phycocyanin ... , 2 types, 168 molecules B1D1F1H1J1L1O1Q1S1U1W1Y1B2D2F2H2J2L2O2Q2S2U2W2Y2B3D3F3H3J3L3...

#2: Protein ...
C-phycocyanin alpha subunit


Mass: 17475.451 Da / Num. of mol.: 84
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Strain: PCC 7120 / Gene: cpcA / Production host: Nostoc sp. PCC 7120 = FACHB-418 (bacteria) / References: UniProt: P07121
#3: Protein ...
C-phycocyanin beta subunit


Mass: 18403.758 Da / Num. of mol.: 84
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Strain: PCC 7120 / Gene: cpcB / Production host: Nostoc sp. PCC 7120 = FACHB-418 (bacteria) / References: UniProt: P07120

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Allophycocyanin subunit ... , 4 types, 94 molecules A8C8E8H8J8L8O8Q8S8V8X8Z8c8e8g8j8l8n8p8r8t8A9C9E9G9I9K9N9P9R9...

#7: Protein ...
Allophycocyanin subunit alpha 1


Mass: 17362.617 Da / Num. of mol.: 44
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Strain: PCC 7120 / Gene: apcA1 / Production host: Nostoc sp. PCC 7120 = FACHB-418 (bacteria) / References: UniProt: P80555
#8: Protein ...
Allophycocyanin subunit beta


Mass: 17320.695 Da / Num. of mol.: 46
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Strain: PCC 7120 / Gene: apcB / Production host: Nostoc sp. PCC 7120 = FACHB-418 (bacteria) / References: UniProt: P80557
#11: Protein Allophycocyanin subunit beta-18 / Allophycocyanin subunit B18


Mass: 18657.016 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Strain: PCC 7120 / Gene: apcF / Production host: Nostoc sp. PCC 7120 = FACHB-418 (bacteria) / References: UniProt: Q7A2D6
#12: Protein Allophycocyanin subunit alpha-B


Mass: 17828.438 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Strain: PCC 7120 / Gene: apcD / Production host: Nostoc sp. PCC 7120 = FACHB-418 (bacteria) / References: UniProt: P80556

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Protein / Non-polymers , 2 types, 350 molecules 0919

#10: Protein Phycobiliprotein ApcE / Anchor polypeptide / PBS-anchor protein / Phycobilisome linker polypeptide


Mass: 127045.812 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Strain: PCC 7120 / Gene: apcE / Production host: Nostoc sp. PCC 7120 = FACHB-418 (bacteria) / References: UniProt: P80559, Lyases
#13: Chemical...
ChemComp-CYC / PHYCOCYANOBILIN


Mass: 588.694 Da / Num. of mol.: 348 / Source method: obtained synthetically / Formula: C33H40N4O6 / Feature type: SUBJECT OF INVESTIGATION

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Cryo-EM structure of cyanobacterial phycobilisome from Anabaena sp. PCC 7120
Type: COMPLEX / Entity ID: #1-#12 / Source: NATURAL
Molecular weightValue: 7 MDa
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Buffer solutionpH: 7
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 %

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingElectron dose: 64 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 62439 / Symmetry type: POINT

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