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- PDB-7eu9: Crystal structure of the selenomethionine(SeMet)-derived Cas12i1 ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7eu9 | ||||||
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Title | Crystal structure of the selenomethionine(SeMet)-derived Cas12i1 R-loop complex before target DNA cleavage | ||||||
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![]() | RNA BINDING PROTEIN / Cas12i / Cas12i1 / CRISPR | ||||||
Function / homology | CITRIC ACID / DNA / DNA (> 10) / RNA / RNA (> 10)![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, B. / Luo, D.Y. / Li, Y. / OuYang, S.Y. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1. Authors: Zhang, B. / Luo, D. / Li, Y. / Perculija, V. / Chen, J. / Lin, J. / Ye, Y. / Ouyang, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 280.4 KB | Display | ![]() |
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PDB format | ![]() | 227.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-DNA chain , 2 types, 2 molecules CD
#3: DNA chain | Mass: 12326.935 Da / Num. of mol.: 1 / Source method: obtained synthetically Source: (synth.) ![]() |
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#4: DNA chain | Mass: 12241.883 Da / Num. of mol.: 1 / Source method: obtained synthetically Source: (synth.) ![]() |
-Protein / RNA chain , 2 types, 2 molecules AB
#1: Protein | Mass: 128172.484 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() |
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#2: RNA chain | Mass: 13781.143 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() |
-Non-polymers , 2 types, 80 molecules ![](data/chem/img/CIT.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | ChemComp-CIT / |
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#6: Water | ChemComp-HOH / |
-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.82 Å3/Da / Density % sol: 53.41 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop Details: 0.1 M sodium citrate (pH 5.6), 17% (w/v) Polyethylene glycol 3350, 0.1 M sodium citrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 30, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→60 Å / Num. obs: 78375 / % possible obs: 100 % / Redundancy: 13.2 % / CC1/2: 0.997 / Rmerge(I) obs: 0.14 / Net I/σ(I): 9.4 |
Reflection shell | Resolution: 2.35→2.48 Å / Rmerge(I) obs: 0.969 / Num. unique obs: 11343 / CC1/2: 0.83 |
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Processing
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Refinement | Method to determine structure: ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 200 Å2 / Biso mean: 58.834 Å2 / Biso min: 27.49 Å2
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Refinement step | Cycle: final / Resolution: 2.35→58.63 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.35→2.411 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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