PDB-7ern: Crystal structure of D-allulose 3-epimerase with D-fructose from ...

ID or keywords:

Entry

Database: PDB / ID: 7ern

Title

Crystal structure of D-allulose 3-epimerase with D-fructose from Agrobacterium sp. SUL3

Components

D-tagatose 3-epimerase

Keywords

ISOMERASE / D-allulose / rare sugar / Agrobacterium sp. SUL3 / D-allulose 3-epimerase

Function / homology

Xylose isomerase-like, TIM barrel domain / Xylose isomerase-like TIM barrel / Xylose isomerase-like superfamily / D-fructose / D-tagatose 3-epimerase

Function and homology information

Biological species

Agrobacterium sp. SUL3 (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.05 Å

Authors

Zhu, Z.L. / Miyakawa, T. / Tanokura, M. / Lu, F.P. / Qin, H.-M.

Citation

Journal: Adv Sci / Year: 2024
Title: Growth-Coupled Evolutionary Pressure Improving Epimerases for D-Allulose Biosynthesis Using a Biosensor-Assisted In Vivo Selection Platform
Authors: Li, C. / Gao, H. / Li, H. / Wang, T. / Lu, F.P. / Qin, H.-M.

History

Deposition	May 6, 2021	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	May 11, 2022	Provider: repository / Type: Initial release
Revision 1.1	Nov 29, 2023	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.2	Feb 7, 2024	Group: Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.title / _citation.year

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	7ern.cif.gz	525.7 KB	Display	PDBx/mmCIF format
PDB format	pdb7ern.ent.gz	359.6 KB	Display	PDB format
PDBx/mmJSON format	7ern.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	7ern_validation.pdf.gz	2.9 MB	Display	wwPDB validaton report
Full document	7ern_full_validation.pdf.gz	2.9 MB	Display
Data in XML	7ern_validation.xml.gz	43.4 KB	Display
Data in CIF	7ern_validation.cif.gz	60.7 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/er/7ern ftp://data.pdbj.org/pub/pdb/validation_reports/er/7ern	HTTPS FTP

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Related structure data

Related structure data	7ermC 7eroC 7dz2S S: Starting model for refinement C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#74 - Feb 2006 alpha-Amylase similarity (3)

Deposited unit

A: D-tagatose 3-epimerase

B: D-tagatose 3-epimerase

C: D-tagatose 3-epimerase

D: D-tagatose 3-epimerase

hetero molecules

123 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software
Evidence: gel filtration

Unit cell

Length a, b, c (Å)	66.191, 97.953, 160.172
Angle α, β, γ (deg.)	90.000, 90.000, 90.000
Int Tables number	19
Space group name H-M	P2₁2₁2₁
Space group name Hall	P2ac2ab
Symmetry operation	#1: x,y,z #2: x+1/2,-y+1/2,-z #3: -x,y+1/2,-z+1/2 #4: -x+1/2,-y,z+1/2

#1: Protein	D-tagatose 3-epimerase Mass: 30585.398 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Agrobacterium sp. SUL3 (bacteria) / Gene: AKG12_23230 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): Rosetta References: UniProt: A0A0L6K0Q2, Isomerases; Racemases and epimerases; Acting on carbohydrates and derivatives
#2: Sugar	ChemComp-FUD / D-fructose Type: D-saccharide / Mass: 180.156 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₆H₁₂O₆ / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#4: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 349 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.15 Å³/Da / Density % sol: 42.82 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.2M Magnesium chloride, 0.1M Tris pH=8.5, 30% PEG 4000

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Data collection

Diffraction	Mean temperature: 95 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-1A / Wavelength: 1 Å
Detector	Type: DECTRIS EIGER X 4M / Detector: PIXEL / Date: Oct 30, 2020
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 2.05→46.88 Å / Num. obs: 66104 / % possible obs: 99.9 % / Redundancy: 6.8 % / B_iso Wilson estimate: 33.81 Å² / CC_1/2: 0.999 / R_pim(I) all: 0.037 / R_rim(I) all: 0.097 / Net I/σ(I): 14.1
Reflection shell	Resolution: 2.05→2.1 Å / Mean I/σ(I) obs: 1.9 / Num. unique obs: 4343 / CC_1/2: 0.75 / R_pim(I) all: 0.43

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Processing

Software

Name	Version	Classification
PHENIX	1.17.1_3660	refinement
XDS		data reduction
Aimless		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 7DZ2

7dz2

Resolution: 2.05→46.84 Å / SU ML: 0.2087 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 22.8156
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2097	3340	5.06 %
R_work	0.1601	62675	-
obs	0.1626	66015	99.8 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 41.11 Å²

Refinement step

Cycle: LAST / Resolution: 2.05→46.84 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	8592	0	40	349	8981

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0069	8820
X-RAY DIFFRACTION	f_angle_d	0.8139	11971
X-RAY DIFFRACTION	f_chiral_restr	0.0486	1313
X-RAY DIFFRACTION	f_plane_restr	0.0047	1574
X-RAY DIFFRACTION	f_dihedral_angle_d	9.4188	1221

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.05-2.08	0.3237	143	0.2539	2576	X-RAY DIFFRACTION	99.13
2.08-2.11	0.3	136	0.24	2576	X-RAY DIFFRACTION	99.96
2.11-2.14	0.2533	110	0.2239	2602	X-RAY DIFFRACTION	99.34
2.14-2.18	0.2898	139	0.2117	2544	X-RAY DIFFRACTION	99.85
2.18-2.22	0.2513	163	0.2039	2551	X-RAY DIFFRACTION	99.49
2.22-2.26	0.2532	134	0.1896	2587	X-RAY DIFFRACTION	100
2.26-2.3	0.3213	135	0.1873	2566	X-RAY DIFFRACTION	99.56
2.3-2.35	0.2443	136	0.1853	2596	X-RAY DIFFRACTION	99.93
2.35-2.4	0.2344	130	0.1888	2585	X-RAY DIFFRACTION	99.78
2.4-2.45	0.2537	132	0.1853	2624	X-RAY DIFFRACTION	99.82
2.45-2.51	0.2255	139	0.183	2588	X-RAY DIFFRACTION	99.89
2.51-2.58	0.2929	134	0.1872	2585	X-RAY DIFFRACTION	99.89
2.58-2.66	0.2245	131	0.1862	2611	X-RAY DIFFRACTION	100
2.66-2.74	0.2372	133	0.198	2606	X-RAY DIFFRACTION	99.96
2.74-2.84	0.2593	120	0.1879	2624	X-RAY DIFFRACTION	99.96
2.84-2.96	0.2624	155	0.1881	2573	X-RAY DIFFRACTION	99.82
2.96-3.09	0.2525	137	0.1771	2607	X-RAY DIFFRACTION	99.82
3.09-3.25	0.2424	147	0.1814	2620	X-RAY DIFFRACTION	99.89
3.25-3.46	0.2174	137	0.1675	2629	X-RAY DIFFRACTION	99.96
3.46-3.72	0.1862	157	0.1388	2609	X-RAY DIFFRACTION	99.86
3.72-4.1	0.1721	146	0.1195	2653	X-RAY DIFFRACTION	99.86
4.1-4.69	0.1349	140	0.1079	2666	X-RAY DIFFRACTION	99.86
4.69-5.91	0.1591	149	0.1318	2683	X-RAY DIFFRACTION	99.86
5.91-46.84	0.1692	157	0.1407	2814	X-RAY DIFFRACTION	99.8

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.158112938165	-0.0895079206006	-0.0683087294669	0.525665867038	-0.559767213398	0.548843751756	0.0110731569135	0.107699414164	0.0574299076004	0.106355482561	-0.0812785153177	0.0775989683004	-0.121570326593	-0.107357772273	5.34877557475E-5	0.311894438802	0.0131073258433	-0.012465590345	0.229567791822	0.0141070777612	0.292610312603	-27.4350476975	22.9633645727	-12.273274945
2	0.569505065413	-0.149434646634	0.0114043560456	0.296940042024	-0.187077586088	0.97070932196	0.0154429119092	0.119455706433	-0.0182652775647	-0.0534947504484	-0.0827815130826	0.00344901625485	0.0723731773268	0.0748474100622	-2.38956383503E-5	0.261424378645	0.0251024820285	0.0243105196411	0.246099280212	-0.0126761581989	0.2357226225	-21.6372466234	4.18994589318	-9.6648802566
3	0.0473172215633	-0.00605430904225	0.0262341259408	0.0401473064976	-0.0326020404711	-0.00222290065873	-0.120609198517	0.205785909732	0.321052507462	-0.145195649215	0.124909794403	0.259263956873	0.0235183250052	-0.258721540743	-0.000476773525944	0.40527662307	0.00440799497324	-0.030625659594	0.395925029331	-0.000998240991733	0.395605275541	-40.8201070633	11.1944821957	-4.79499019198
4	0.10230726682	-0.00264692424243	-0.137441576759	0.0489203805721	-0.150755525121	0.261719175131	-0.226094542103	-0.0726178231661	0.367331426422	0.148086841578	-0.0530416220776	-0.111635093	-0.455046457227	0.107142074936	0.000339368443654	0.322201305719	-0.00294395244984	-0.0347048443191	0.209024753646	0.00663732165577	0.278912896345	-24.0624857799	19.2957937849	7.27044922717
5	0.568320523571	0.245184975947	0.00943209228187	0.346439591934	-0.0830435217717	0.0424569257941	0.0490573601967	-0.323969746173	-0.852632950662	0.230961049773	-0.296477566061	-0.250535997117	0.128085279944	0.164327172409	-0.0156435516497	0.334095039206	0.0314888985807	0.00111377049887	0.397146875269	0.156471157386	0.554786062847	-1.51884052857	-13.9649117852	32.7246427414
6	0.444814432239	0.380292146786	-0.300724427251	0.641008080641	-0.284174407744	0.204645537368	0.0734329522208	-0.290109599457	-0.0934977778705	0.117649227128	-0.195779006037	-0.146515620274	-0.096848498563	0.281502158664	-4.28238099692E-5	0.227357194419	-0.0303696991862	-0.0397548105606	0.408084439228	0.0756817963648	0.321852993859	2.82018365106	6.3628044296	33.4152470597
7	0.0801968427432	0.0481741087548	0.114450846465	0.200526848609	-0.0621308649755	0.130010124621	0.127683721414	-0.185050726235	0.0966215484556	0.0188579635585	-0.164132961632	0.0884644887882	-0.0598390879844	0.0488321575652	-0.000169839287898	0.217547006355	-0.000670110809594	-0.00499287733406	0.285011624561	0.0370339065226	0.25691405658	-3.18024509947	8.65063311171	23.9572313111
8	0.350163218685	0.0359576199425	0.257665713246	0.536404493207	0.0291805123113	0.207178393288	0.00024349683076	-0.0893844454813	-0.0134959107456	0.0279527621845	-0.0475960714901	-0.0380353569984	0.0209281289733	-0.0158348679334	1.79955081593E-5	0.199328929036	0.0157569645989	0.00221367053295	0.247178443849	0.00896907218587	0.237282783536	-13.074795338	-0.289849568063	25.5976059904
9	0.158753615933	-0.0903186067366	0.0942450783139	0.0817316608281	0.0215131250007	0.0991334235912	0.0845495764274	0.00138671836427	-0.164316928326	-0.0879557845637	0.00842158830269	-0.0619744854849	0.525491750286	0.0238796816642	-0.000954936621805	0.377526980608	0.0385267967413	0.026148512547	0.251267028329	-0.00734389978263	0.323471816905	-15.3711133454	-10.4752366193	18.4606523893
10	0.149738540551	0.11736293222	0.0396776451803	0.155399143929	-0.0985700309172	0.167688396298	0.0514514476382	0.124165016085	-0.125904904579	-0.427871543569	-0.0514436714815	-0.0676228719266	0.39246938068	-0.0329511885825	-0.000825383538516	0.513178596411	-0.0700042990116	-0.00349118998489	0.311247246404	-0.00728888748907	0.348526331514	-37.8582385618	-25.2402287285	8.81201831388
11	0.02926623717	-0.115718419627	0.0156104769565	0.170425955032	0.0167620749431	0.0134081330252	0.00454854059934	-0.0703315301536	0.0479092403672	-0.0622106121193	-0.0257485545946	0.1547047896	0.277958245975	-0.232545766419	-4.30381962176E-5	0.52481071272	-0.17444006591	-0.0775997503118	0.346165037927	0.0747406442004	0.400035904464	-44.2163399944	-27.4493013992	9.16785735807
12	0.618104436449	-0.0283797548006	0.0554192977601	0.298721952779	0.26592268224	0.22037409583	0.14469025921	-0.0241613881001	-0.0702944663805	-0.350680965474	0.0302858245354	0.377179686402	0.344833095865	-0.45744243947	0.000148450231557	0.35854939557	-0.125301517952	-0.104080425161	0.420769192187	0.0178868834518	0.402380553646	-51.4140756704	-13.5940723317	-0.0329268446287
13	0.0844295264543	-0.0760508242781	-0.012079494505	0.226507353491	0.141706456186	0.203029461715	0.158569709428	-0.201827116827	-0.0659174792646	-0.0919789568245	-0.209429912273	0.182360757126	-0.0791132297729	-0.285047492244	-0.00302659064898	0.248415582725	-0.0752287906295	-0.0513681896978	0.448769787505	-0.0136164012269	0.385061616542	-53.5608407472	-6.7481731956	2.59343723328
14	0.0110503999061	-0.0100126856552	0.0133127138465	0.0709953101045	0.107215102458	0.47025267523	-0.00671400320533	-0.157792192926	-0.0289046219503	0.0381587548761	-0.211238318302	0.268143629722	-0.406758590547	-0.202448510027	-0.0300317126584	0.188637550115	-0.00708624301599	0.0134790132041	0.412090461602	-0.032235645423	0.46789497688	-55.6541769814	-1.80618125597	9.20876818774
15	0.152122427245	-0.0148447391793	0.122708083942	0.0485037366653	0.0469462826466	0.211775011346	0.0253480030003	-0.0216406273504	-0.0441439510366	-0.011908969426	-0.0150778211925	0.00784376718093	0.0322674953107	-0.0727755370384	-3.66167973052E-6	0.256695254173	-0.0304445784607	0.0112326287792	0.241659644972	0.00520288821741	0.259093840192	-38.9123045539	-4.13919363555	10.9748396651
16	0.0203300036863	-0.0123661853794	-0.00769005580185	0.0193184003362	-0.0226894522876	0.0355749459082	-0.0663506405678	-0.425846472215	-0.181583317035	0.210329900715	-0.00993006779506	0.0196152294823	0.187621070738	0.0372125733709	-0.000124844937446	0.246180580734	-0.0361791516231	0.0243904192235	0.297254663992	0.0247221388774	0.288349623759	-38.4912327899	-6.22933716582	19.5086839544
17	0.0222048245545	0.0134231860864	-0.026689932707	0.00142000170987	-0.0168877013629	0.0445122848383	-0.193167128272	0.309796175562	-0.33755333167	-0.114459937359	0.0494085170889	0.00758581851335	0.330997802181	0.0152677410118	-0.000333967945749	0.532294331561	-0.0160870639739	0.0219274186439	0.378031907344	-0.0342663216803	0.366624447694	-30.19211063	-14.9774726427	-2.21561953353
18	0.170491808216	0.128177474445	0.0463882146629	0.125254439584	-0.0137458188608	0.0629084142571	0.134176060832	-0.187905977018	-0.197340301134	0.202975520415	-0.138349043132	-0.0383144989461	0.285268315393	0.0178156575282	-0.000655917401436	0.359694742835	-0.0221296329923	-0.026101688031	0.250633112779	0.0382951526923	0.291594019694	-30.9953904605	-12.8872728075	20.6052541787
19	0.228483509892	0.0489494598661	-0.0281509182113	0.0331291387515	-0.0252825501236	0.107613392178	0.12149863949	-0.140054806121	0.312945793486	-0.19001767787	-0.17626759374	0.16934343985	-0.420425976294	-0.15643216406	-0.000895353638068	0.429388447009	0.0447484514405	0.0768127310661	0.199595371417	-0.0830042518107	0.393605635864	-31.0756108009	31.3946436667	31.8905951857
20	0.0916814430295	-0.00473466214386	-0.101551035129	0.235716007784	-0.0128830223654	0.0823772334406	0.198993526548	-0.157556379398	0.395741648612	-0.0386441864634	-0.0397058315493	-0.0840848630599	-0.0320186829949	0.0859269831894	-0.000143906556275	0.352008790389	0.0680845092248	0.0135273440401	0.34007506149	-0.0932469244111	0.386784031535	-35.3949347298	32.6581021806	36.8547626455
21	0.417298352867	0.337761159329	-0.0285535227148	0.286199966977	0.0401021439587	0.151693688456	-0.0465277339809	-0.334687225944	0.155926805396	0.170711955602	0.0324032638071	-0.145908228352	-0.101048884474	-0.121741110289	-4.24052985313E-5	0.353519826138	0.0537564930703	-0.00420308101362	0.389964749826	-0.0874082012104	0.294895082869	-30.1884834846	18.5810280859	47.1989169214
22	0.163938766894	0.148073882875	-0.0538854090901	0.144516441566	-0.0332618646562	-0.00948940746151	0.0566596027789	-0.465812402804	-0.0830280996272	0.333959789367	-0.164125562993	0.0617707150242	0.0635029634439	-0.369445984809	-0.00499690573286	0.321702398845	0.0349068565318	0.0382154264549	0.646525094704	-0.0391243814928	0.283935824866	-36.5742005791	11.8855477584	52.3759527905
23	0.0378692411431	0.0614253235061	0.0547369994943	0.117994577293	0.0807083571335	0.0904317719163	0.0620159464842	-0.179973776219	-0.00319935298203	0.0663806317808	-0.0941249199762	-0.0270460983668	-0.137016359812	-0.165192810788	8.75580539335E-6	0.28163637129	0.0147764794286	0.000547567247954	0.357580431801	-0.0339554384348	0.260333218546	-28.4982339753	10.9165671142	41.0250039759
24	0.0769993399707	-0.0461752112296	0.127436412882	0.191013686988	0.0743433590875	0.185071031156	-0.0626085426708	-0.121924166805	-0.0882985930775	0.0708734659837	-0.03325702187	-0.107216154262	0.1112481432	-0.146764769104	6.23411583502E-5	0.251292620657	-0.0125142616075	0.00226360939786	0.3126072873	-0.00605978722696	0.23914427715	-33.8454249267	8.08260037334	38.9018022341
25	0.0274393995283	0.0129589393443	-0.0425346201185	-0.0088191770207	-0.021433287477	0.0509842902638	0.132854492919	-0.0565669171167	0.0358999072171	0.111819330567	-0.159770598321	-0.0873348374106	-0.0867263657742	0.0430824340633	9.78065942641E-5	0.249984255511	0.000778489824188	0.0300278310339	0.318641345943	-0.0236807593746	0.256506156629	-35.6191027547	7.32150181409	31.7111876323
26	0.00629064960208	-0.0183503905864	-0.00643606289438	0.0380679213545	0.0267537748629	0.00559295369295	0.25351579327	0.197589947358	0.10101533673	-0.0504840097149	0.00143469870207	-0.103469237845	-0.266051135089	0.105208430735	-4.15908934458E-5	0.287344170887	0.0110643446142	0.00564603362376	0.289643859524	-0.0185238107279	0.305913776343	-23.1663567496	14.7719602979	22.9005225851
27	0.0631541615045	0.00153530506153	0.0477744755795	0.0633721157915	-0.0617068647317	0.0725920934059	0.00711412660054	-0.160102826324	0.0700991315402	-0.0837784869149	-0.0238563947033	0.276964518657	-0.0668727894225	-0.0898585649205	0.000387668115883	0.246350010016	0.0230368105465	0.019231635611	0.28347225178	-0.0259665207974	0.271135452065	-37.2875888743	11.6588246391	24.8621226343
28	0.0258071414412	0.0382969621151	0.0337994902766	0.0437584822319	0.0296765377496	0.00991695088344	0.15382160242	0.0879976544993	0.355958625266	-0.299608706481	-0.172979904439	-0.00531557572905	-0.153305986593	0.223445519564	-0.000299927380775	0.427296324153	-0.0537895339455	0.00812681424848	0.3571393813	-0.0239342770029	0.363918988882	-16.231080191	23.1894013813	31.5401842462
29	0.0680039481207	-0.00251317219426	0.0852181809825	0.0784394321142	-0.103788315699	0.131786483866	-0.044408756097	0.0489635277637	0.0126729198212	-0.331953467052	-0.0545583663647	0.0797192810876	-0.240200125761	-0.0366303487093	7.38681367136E-5	0.33138549096	0.0485993804776	-0.0251774297098	0.277732406782	-0.0428990627834	0.310213580286	-33.9855481846	19.5293597581	18.5056834543

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 1 through 66 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 67 through 237 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 238 through 260 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 261 through 283 )
5	X-RAY DIFFRACTION	5	chain 'B' and (resid 1 through 57 )
6	X-RAY DIFFRACTION	6	chain 'B' and (resid 58 through 156 )
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 157 through 184 )
8	X-RAY DIFFRACTION	8	chain 'B' and (resid 185 through 260 )
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 261 through 283 )
10	X-RAY DIFFRACTION	10	chain 'C' and (resid 1 through 29 )
11	X-RAY DIFFRACTION	11	chain 'C' and (resid 30 through 57 )
12	X-RAY DIFFRACTION	12	chain 'C' and (resid 58 through 116 )
13	X-RAY DIFFRACTION	13	chain 'C' and (resid 117 through 156 )
14	X-RAY DIFFRACTION	14	chain 'C' and (resid 157 through 171 )
15	X-RAY DIFFRACTION	15	chain 'C' and (resid 172 through 218 )
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 219 through 237 )
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 238 through 260 )
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 261 through 284 )
19	X-RAY DIFFRACTION	19	chain 'D' and (resid 1 through 29 )
20	X-RAY DIFFRACTION	20	chain 'D' and (resid 30 through 57 )
21	X-RAY DIFFRACTION	21	chain 'D' and (resid 58 through 116 )
22	X-RAY DIFFRACTION	22	chain 'D' and (resid 117 through 137 )
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 138 through 156 )
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 157 through 184 )
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 185 through 204 )
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 205 through 218 )
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 219 through 237 )
28	X-RAY DIFFRACTION	28	chain 'D' and (resid 238 through 260 )
29	X-RAY DIFFRACTION	29	chain 'D' and (resid 261 through 283 )

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