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Open data
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Basic information
Entry | Database: PDB / ID: 7eme | ||||||
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Title | Putative Leptospira interrogans recombinant L-amino acid oxidase | ||||||
![]() | NAD(P)/FAD-dependent oxidoreductase | ||||||
![]() | OXIDOREDUCTASE / Leptospira interrogans / L-amino acid oxidase / FAD-dependent oxidoreductase / Flavoprotein / Putative amine oxidase | ||||||
Function / homology | Flavin amine oxidase / Amine oxidase / Flavin containing amine oxidoreductase / FAD/NAD(P)-binding domain superfamily / oxidoreductase activity / FLAVIN-ADENINE DINUCLEOTIDE / NAD(P)/FAD-dependent oxidoreductase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Vaigundan, D. / Yuvaraj, I. / Krishnaswamy, P.R. / Sekar, K. / Murthy, M.R.N. / Sunita, P. | ||||||
![]() | ![]() Title: Structural characterization of a putative recombinant L-amino acid oxidase from Leptospira interrogans Authors: Vaigundan, D. / Yuvaraj, I. / Sunita, P. / Sekar, K. / Murthy, M.R.N. / Krishnaswamy, P.R. #1: ![]() Title: Structural characterization of a putative recombinant L-amino acid oxidase from Leptospira interrogans Authors: Vaigundan, D. / Krishnaswamy, P.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 188.3 KB | Display | ![]() |
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PDB format | ![]() | 145 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 897.5 KB | Display | ![]() |
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Full document | ![]() | 900.5 KB | Display | |
Data in XML | ![]() | 37.1 KB | Display | |
Data in CIF | ![]() | 57.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 49260.070 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.95 Å3/Da / Density % sol: 36.99 % |
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Crystal grow | Temperature: 298 K / Method: microbatch Details: 0.1 M Potassium thiocyanate, 30% w/v Polyethylene glycol monomethyl ether 2000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 3, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0723 Å / Relative weight: 1 |
Reflection | Resolution: 1.78→84.61 Å / Num. obs: 74369 / % possible obs: 99.1 % / Redundancy: 11.3 % / Rmerge(I) obs: 0.118 / Rpim(I) all: 0.037 / Rrim(I) all: 0.124 / Net I/σ(I): 14.3 |
Reflection shell | Resolution: 1.78→1.81 Å / Rmerge(I) obs: 0.39 / Num. unique obs: 4197 / CC1/2: 0.978 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 73.44 Å2 / Biso mean: 15.409 Å2 / Biso min: 6.51 Å2
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Refinement step | Cycle: final / Resolution: 1.78→70.06 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.78→1.824 Å / Rfactor Rfree error: 0
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