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- PDB-7ehl: Cryo-EM structure of human ABCB8 transporter in nucleotide bindin... -
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Basic information
Entry | Database: PDB / ID: 7ehl | ||||||
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Title | Cryo-EM structure of human ABCB8 transporter in nucleotide binding state | ||||||
![]() | Mitochondrial potassium channel ATP-binding subunit | ||||||
![]() | MEMBRANE PROTEIN / ABC transporter | ||||||
Function / homology | ![]() mitochondrial ATP-gated potassium channel complex / mitochondrial potassium ion transmembrane transport / ABC-type peptide transporter activity / Mitochondrial ABC transporters / cell volume homeostasis / potassium ion transmembrane transport / ATP-binding cassette (ABC) transporter complex / mitochondrial membrane / transmembrane transport / mitochondrial inner membrane ...mitochondrial ATP-gated potassium channel complex / mitochondrial potassium ion transmembrane transport / ABC-type peptide transporter activity / Mitochondrial ABC transporters / cell volume homeostasis / potassium ion transmembrane transport / ATP-binding cassette (ABC) transporter complex / mitochondrial membrane / transmembrane transport / mitochondrial inner membrane / nucleolus / ATP hydrolysis activity / mitochondrion / nucleoplasm / ATP binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM | ||||||
![]() | Li, S.J. / Yang, X. / Shen, Y.Q. | ||||||
![]() | ![]() Title: Cryo-EM structure of human ABCB8 transporter in nucleotide binding state. Authors: Shunjin Li / Yue Ren / Xuhang Lu / Yuequan Shen / Xue Yang / ![]() Abstract: Human ATP-binding cassette transporter 8 of subfamily B (hABCB8) is an ABC transporter that located in the inner membrane of mitochondria. The ABCB8 is involved in the maturation of Fe-S and protects ...Human ATP-binding cassette transporter 8 of subfamily B (hABCB8) is an ABC transporter that located in the inner membrane of mitochondria. The ABCB8 is involved in the maturation of Fe-S and protects the heart from oxidative stress. Here, we present the cryo-EM structure of human ABCB8 binding with AMPPNP in inward-facing conformation with resolution of 4.1 Å. hABCB8 shows an open-inward conformation when ATP is bound. Unexpectedly, cholesterol molecules were identified in the transmembrane domain of hABCB8. Our results provide structural basis for the transport mechanism of the ABC transporter in mitochondria. | ||||||
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Structure visualization
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Structure viewer | Molecule: ![]() ![]() |
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PDBx/mmCIF format | ![]() | 199.8 KB | Display | ![]() |
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PDB format | ![]() | 152.7 KB | Display | ![]() |
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-Validation report
Summary document | ![]() | 734.2 KB | Display | ![]() |
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Full document | ![]() | 765 KB | Display | |
Data in XML | ![]() | 28.6 KB | Display | |
Data in CIF | ![]() | 44.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 31142MC M: map data used to model this data C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 73628.375 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-CLR / #3: Chemical | #4: Chemical | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: ABCB8 / Type: CELL / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 6.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 56 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
CTF correction | Type: NONE |
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3D reconstruction | Num. of particles: 43131 / Symmetry type: POINT |