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- PDB-7cvq: crystal structure of Arabidopsis CO CCT domain in complex with NF... -

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Basic information

Entry
Database: PDB / ID: 7cvq
Titlecrystal structure of Arabidopsis CO CCT domain in complex with NF-YB2/YC3 and FT CORE1 DNA
Components
  • Chimera of Nuclear transcription factor Y subunit C-3 and Zinc finger protein CONSTANS
  • FT CORE1 DNA forward strand
  • FT CORE1 DNA reverse strand
  • Nuclear transcription factor Y subunit B-2
KeywordsDNA BINDING PROTEIN/DNA / CONSTANS / transcription factor / DNA BINDING PROTEIN-DNA complex
Function / homology
Function and homology information


far-red light signaling pathway / positive regulation of photomorphogenesis / long-day photoperiodism, flowering / CCAAT-binding factor complex / regulation of flower development / gibberellic acid mediated signaling pathway / regulation of seed germination / flower development / response to far red light / response to water deprivation ...far-red light signaling pathway / positive regulation of photomorphogenesis / long-day photoperiodism, flowering / CCAAT-binding factor complex / regulation of flower development / gibberellic acid mediated signaling pathway / regulation of seed germination / flower development / response to far red light / response to water deprivation / abscisic acid-activated signaling pathway / plastid / transcription coregulator activity / DNA-binding transcription activator activity, RNA polymerase II-specific / cell differentiation / transcription cis-regulatory region binding / DNA-binding transcription factor activity / protein heterodimerization activity / DNA binding / zinc ion binding / nucleus
Similarity search - Function
CCT domain / Zinc finger protein CONSTANS-like / CCT motif / CCT domain profile. / : / Transcription factor, NFYB/HAP3, conserved site / Transcription factor NFYB/HAP3 / NF-YB/HAP3 subunit signature. / : / Transcription factor CBF/NF-Y/archaeal histone domain ...CCT domain / Zinc finger protein CONSTANS-like / CCT motif / CCT domain profile. / : / Transcription factor, NFYB/HAP3, conserved site / Transcription factor NFYB/HAP3 / NF-YB/HAP3 subunit signature. / : / Transcription factor CBF/NF-Y/archaeal histone domain / Histone-like transcription factor (CBF/NF-Y) and archaeal histone / B-box zinc finger / B-Box-type zinc finger / B-box-type zinc finger / Zinc finger B-box type profile. / Histone H2A/H2B/H3 / Core histone H2A/H2B/H3/H4 / Histone-fold
Similarity search - Domain/homology
DNA / DNA (> 10) / Zinc finger protein CONSTANS / Nuclear transcription factor Y subunit B-2 / Nuclear transcription factor Y subunit C-3
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.3 Å
AuthorsLv, X. / Du, J.
CitationJournal: Plant Cell / Year: 2021
Title: Structural insights into the multivalent binding of the Arabidopsis FLOWERING LOCUS T promoter by the CO-NF-Y master transcription factor complex.
Authors: Lv, X. / Zeng, X. / Hu, H. / Chen, L. / Zhang, F. / Liu, R. / Liu, Y. / Zhou, X. / Wang, C. / Wu, Z. / Kim, C. / He, Y. / Du, J.
History
DepositionAug 26, 2020Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jun 16, 2021Provider: repository / Type: Initial release
Revision 1.1Nov 29, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Chimera of Nuclear transcription factor Y subunit C-3 and Zinc finger protein CONSTANS
B: Nuclear transcription factor Y subunit B-2
D: FT CORE1 DNA forward strand
E: FT CORE1 DNA reverse strand
F: Chimera of Nuclear transcription factor Y subunit C-3 and Zinc finger protein CONSTANS
K: Chimera of Nuclear transcription factor Y subunit C-3 and Zinc finger protein CONSTANS
P: Chimera of Nuclear transcription factor Y subunit C-3 and Zinc finger protein CONSTANS
G: Nuclear transcription factor Y subunit B-2
L: Nuclear transcription factor Y subunit B-2
Q: Nuclear transcription factor Y subunit B-2
I: FT CORE1 DNA forward strand
N: FT CORE1 DNA forward strand
T: FT CORE1 DNA forward strand
J: FT CORE1 DNA reverse strand
O: FT CORE1 DNA reverse strand
U: FT CORE1 DNA reverse strand


Theoretical massNumber of molelcules
Total (without water)186,21116
Polymers186,21116
Non-polymers00
Water00
1
A: Chimera of Nuclear transcription factor Y subunit C-3 and Zinc finger protein CONSTANS
B: Nuclear transcription factor Y subunit B-2
D: FT CORE1 DNA forward strand
E: FT CORE1 DNA reverse strand


Theoretical massNumber of molelcules
Total (without water)46,5534
Polymers46,5534
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11520 Å2
ΔGint-79 kcal/mol
Surface area17740 Å2
MethodPISA
2
F: Chimera of Nuclear transcription factor Y subunit C-3 and Zinc finger protein CONSTANS
G: Nuclear transcription factor Y subunit B-2
I: FT CORE1 DNA forward strand
J: FT CORE1 DNA reverse strand


Theoretical massNumber of molelcules
Total (without water)46,5534
Polymers46,5534
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11410 Å2
ΔGint-80 kcal/mol
Surface area18140 Å2
MethodPISA
3
K: Chimera of Nuclear transcription factor Y subunit C-3 and Zinc finger protein CONSTANS
L: Nuclear transcription factor Y subunit B-2
N: FT CORE1 DNA forward strand
O: FT CORE1 DNA reverse strand


Theoretical massNumber of molelcules
Total (without water)46,5534
Polymers46,5534
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10270 Å2
ΔGint-75 kcal/mol
Surface area18630 Å2
MethodPISA
4
P: Chimera of Nuclear transcription factor Y subunit C-3 and Zinc finger protein CONSTANS
Q: Nuclear transcription factor Y subunit B-2
T: FT CORE1 DNA forward strand
U: FT CORE1 DNA reverse strand


Theoretical massNumber of molelcules
Total (without water)46,5534
Polymers46,5534
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10900 Å2
ΔGint-83 kcal/mol
Surface area18530 Å2
MethodPISA
Unit cell
Length a, b, c (Å)103.244, 65.273, 138.284
Angle α, β, γ (deg.)90.000, 91.170, 90.000
Int Tables number4
Space group name H-MP1211

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Components

#1: Protein
Chimera of Nuclear transcription factor Y subunit C-3 and Zinc finger protein CONSTANS / AtNF-YC-3


Mass: 20021.914 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Arabidopsis thaliana (thale cress)
Gene: NFYC3, At1g54830, F14C21.41, T22H22.22, CO, At5g15840, F14F8_220
Plasmid: pET-Sumo / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q9ZVL3, UniProt: Q39057
#2: Protein
Nuclear transcription factor Y subunit B-2 / AtNF-YB-2 / Transcriptional activator HAP3B


Mass: 11175.779 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: NFYB2, HAP3B, At5g47640, MNJ7.23 / Plasmid: pET-Sumo / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q9FGJ3
#3: DNA chain
FT CORE1 DNA forward strand


Mass: 7762.034 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) Arabidopsis thaliana (thale cress)
#4: DNA chain
FT CORE1 DNA reverse strand


Mass: 7592.922 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) Arabidopsis thaliana (thale cress)

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.5 Å3/Da / Density % sol: 50.83 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 9.5 / Details: 0.1 M CHES, pH 9.5 and 40% PEG 400

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.9792 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 16, 2019
RadiationMonochromator: LN2-cooled DCM with Si(111) crystals / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 3.3→40 Å / Num. obs: 28108 / % possible obs: 99.2 % / Redundancy: 4.4 % / Biso Wilson estimate: 95.43 Å2 / CC1/2: 0.996 / CC star: 0.999 / Rmerge(I) obs: 0.148 / Rsym value: 0.148 / Net I/σ(I): 7.7
Reflection shellResolution: 3.3→3.42 Å / Redundancy: 4.1 % / Rmerge(I) obs: 0.811 / Mean I/σ(I) obs: 1 / Num. unique obs: 2727 / CC1/2: 0.872 / CC star: 0.965 / Rsym value: 0.811 / % possible all: 97.4

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Processing

Software
NameVersionClassification
PHENIX1.9_1692refinement
HKL-3000data scaling
PDB_EXTRACT3.25data extraction
HKL-3000data reduction
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7CVO

7cvo


Resolution: 3.3→35.181 Å / SU ML: 0.5 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 31.25 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2642 1411 5.08 %
Rwork0.2412 26377 -
obs0.2425 27788 98.75 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 272.9 Å2 / Biso mean: 127.4662 Å2 / Biso min: 36.28 Å2
Refinement stepCycle: final / Resolution: 3.3→35.181 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6834 4076 0 0 10910
Num. residues----1031
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00611506
X-RAY DIFFRACTIONf_angle_d1.00816327
X-RAY DIFFRACTIONf_chiral_restr0.0431823
X-RAY DIFFRACTIONf_plane_restr0.0061382
X-RAY DIFFRACTIONf_dihedral_angle_d25.8114703
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
3.3001-3.41790.30441290.3137255296
3.4179-3.55470.35921370.2941258598
3.5547-3.71630.28961380.2868262399
3.7163-3.9120.28761170.2709263399
3.912-4.15670.28221380.2659264899
4.1567-4.4770.25591380.23542645100
4.477-4.92650.26641440.2262674100
4.9265-5.63690.2671480.25262999
5.6369-7.09230.30141470.2592267799
7.0923-350.21891750.1933271198
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.59710.376-0.83951.2408-1.27592.0395-0.28820.22550.8955-1.00290.22940.2175-0.47961.3154-0.08561.24850.26310.171.1074-0.27251.417764.00133.10344.267
20.0267-0.07910.09570.1259-0.5864.7392-0.08370.3521-0.205-0.34110.7912-0.40510.5495-1.20790.07471.799-0.27560.2666-0.05410.29370.646345.61324.78350.102
35.87770.9669-4.11010.2231-0.6652.8643-0.1843-0.78320.32240.1973-0.05190.91830.8428-1.11740.07731.551-0.1303-0.0551.4201-0.01420.934832.36525.42560.977
43.98372.41520.01862.9351.10680.8325-0.2004-0.2167-0.27420.78950.266-0.3710.1576-0.1337-0.07880.78120.05-0.00361.5547-0.01530.922423.31331.49954.723
53.7343-0.77340.76893.3482-1.21964.18990.3611-0.7847-0.2256-1.140.56030.2455-1.306-0.4185-0.03090.7630.14780.0410.65020.0710.51943.64439.53531.659
64.74591.2125-0.69522.15621.07021.49540.82490.6611.8308-0.58190.16330.66-0.5617-0.86570.27181.47350.09220.3167-0.09491.2534-0.276746.62847.23419.456
71.8536-0.27781.51041.4309-1.21653.15430.5924-0.1291-0.23440.24730.3813-0.57370.2046-0.0481-0.15541.070.06240.05340.4681-0.01680.573253.23936.40940.665
80.8263-1.19661.64961.9875-2.63773.6639-0.5015-0.4514-0.53360.33390.7791-0.3154-0.29560.22310.01471.3978-0.0236-0.14220.8508-0.10140.720752.14238.62860.009
91.7228-0.3479-1.37921.35430.4671.1245-0.1668-0.35390.08111.4922-0.1213-1.1918-0.02821.174-1.99882.2128-0.4865-0.31110.5328-0.5941-0.116456.82145.33649.095
102.3893-0.3191-0.47963.9802-1.55860.9194-0.6834-0.0269-0.0665-1.79510.3384-0.2571-0.8732-0.2581-2.43991.5792-0.0113-0.05250.3129-0.27370.730750.01452.79546.934
115.6003-1.15131.57892.45691.34291.72841.9611-0.0101-1.60530.7640.0960.3378-0.3358-0.72880.62851.133-0.07480.27080.49260.21390.229943.29736.94447.223
122.21870.836-0.85560.4161-1.12835.2492-0.44420.052-0.4499-0.4845-0.4418-0.5169-0.166-0.1038-0.09191.4940.1160.20450.43110.05150.631652.36946.36129.995
134.06541.69990.40562.6017-0.87227.19331.06870.8136-0.8205-0.2060.6887-1.5475-0.84991.11680.13331.29110.0130.2820.5741-0.17790.736861.638.4830.119
14-0.0350.3577-0.56751.26310.08282.91740.1284-0.18830.25790.9665-0.1515-0.0279-0.3975-0.70830.05781.46980.32650.10711.0074-0.02270.583742.639.08768.918
151.57190.306-0.5366-0.1831-0.53213.77140.44570.4983-0.0036-0.49980.35730.6417-0.3972-2.05720.00561.3831-0.04680.0411.22650.09060.829329.83432.66731.601
160.7632-0.0865-1.32750.3223-0.78744.36470.00110.15650.0529-0.61530.30630.25760.7776-1.5990.01011.53910.0604-0.0151.19020.03220.747731.81235.51129.695
171.6714-0.575-0.6810.00480.0531.2750.5971-1.261-0.03891.70820.02990.1662-0.3457-0.31820.08181.7760.3030.06110.84490.09590.807140.638.77167.409
184.7001-4.02841.63873.9451-0.10575.60611.21450.66190.046-0.3813-0.60280.33710.54140.992-0.01811.1787-0.07440.03740.4738-0.0640.886366.74920.94625.999
191.8425-0.6870.38330.6029-0.13122.4788-0.59590.1173-0.4081-1.16590.1281-0.3595-0.1851-1.981-0.0460.9960.4193-0.10930.8830.05320.77937.90817.8413.859
20-0.00330.04790.00170.0408-0.06130.12470.7546-0.33640.1758-0.36240.89330.621-0.5277-0.02560.05842.70740.3039-0.09021.427-0.13421.3937-16.44530.9349.955
21-0.0104-0.0180.02350.00090.0013-0.010.6265-0.17520.53020.57270.1676-0.9429-0.4899-0.78910.0612.2271-0.36130.06261.59840.21451.32183.34627.56457.021
224.91544.5308-3.31057.9736-4.1652.5716-1.2637-0.6472-1.0583-0.0964-0.311-1.63880.120.0265-0.85322.4787-0.1598-0.29183.0931-1.27731.283714.2118.42569.288
230.03620.0627-0.02450.07780.0934-0.0065-0.0735-0.03040.79960.6045-0.32951.9447-1.0908-0.79790.05741.8596-1.1081-0.00162.9909-0.15761.7086-3.51232.33218.862
241.5073-1.15940.20621.01570.59061.45340.3429-0.2711-0.6485-0.6008-0.3281-0.9973-0.35060.46940.04221.31840.004-0.00431.21620.01960.6722.99744.1916.704
252.97831.5375-0.56451.961-1.13222.927-0.4075-0.64230.1820.8912-0.0470.2943-1.0333-0.8981-0.01591.07480.0958-0.01630.5467-0.04820.520748.8397.63937.182
262.6571-0.1136-0.90221.52450.55223.7490.85910.5722-0.1333-0.2826-0.3074-0.0347-0.021-0.18850.28991.55940.46640.01750.43490.34830.406553.7520.77649.222
271.683-0.1839-0.44742.012-0.17133.68280.1907-0.07890.4247-0.28250.2077-0.198-0.56381.1652-0.02241.007-0.17250.1080.4456-0.00960.394357.68113.91928.659
284.18344.0018-5.50235.74-3.45079.0067-0.0810.58831.6459-1.57930.91370.098-0.54571.0160.14971.24630.0650.06620.74060.06930.62957.77612.2269.174
295.19220.05530.32722.4341-0.35551.24830.070.54350.0864-1.740.7212-0.3730.63661.63240.01670.93680.1178-0.05660.6505-0.17710.577163.8616.41620.013
306.67342.2002-1.34082.19750.1823.1692-0.61181.41340.22210.92660.2238-0.42781.4070.1926-0.18770.94090.23060.12770.32510.08610.770759.042-2.71120.999
311.14180.2441-0.7291.4488-0.63551.8717-0.1376-0.0418-0.21340.34440.37190.04070.4085-0.03070.00580.90280.07050.11581.6753-0.17210.763-6.65216.91342.334
320.54830.15830.11311.44211.21171.9256-0.2888-0.9977-0.3573-0.2526-0.06931.1058-0.8945-0.92680.10821.436-0.03690.4651.58810.12571.2573-15.38712.4658.054
330.09660.575-0.03714.56381.150.27180.1945-0.7250.3197-0.23970.9846-1.87950.1141-1.6220.21831.36160.4527-0.02581.67370.17460.59487.51253.11833.282
340.77220.03930.56151.104-0.13450.70120.2845-0.0961-0.5820.94220.09991.19790.1745-0.83781.53091.4447-1.197-1.23653.43460.0162-1.20733.24642.27517.196
350.5412-0.5137-0.06030.7180.29660.26230.85980.1197-1.3434-0.75790.4579-0.12340.7024-0.5250.39171.787-1.072-0.4733.709-0.4805-0.0413-5.23747.90112.564
363.5083-2.2112-1.94611.97031.64111.29970.95740.36593.3316-0.41570.2648-0.9393-0.2857-1.07670.1231.39410.8391-0.57432.1170.2731.0601-3.78257.88713.272
372.50542.28110.03082.89540.33322.78330.1378-1.03480.24380.6266-0.41021.09561.3287-2.1418-0.10140.57820.0644-0.24610.63040.11750.488146.4797.25225.171
384.72711.94551.93369.50747.21075.62780.0588-0.02950.4358-0.79620.5284-1.46570.5291-0.58620.1040.79190.0404-0.02270.50740.2160.570152.55817.67215.377
391.95180.45120.5580.185-0.60556.7527-0.3831-0.49120.25180.3143-0.2878-0.11130.5473-0.1197-0.00291.05560.13-0.04180.5266-0.06560.483459.2833.73539.133
403.86581.7596-0.94120.84380.15724.017-0.6445-0.3169-0.85810.23180.2588-1.06990.36510.9443-0.07331.2732-0.0439-0.07190.6588-0.02430.706266.31911.72739.375
414.937-2.1774-2.09195.3945-0.63679.9869-1.2502-0.46650.99420.2886-0.3108-0.2987-0.52810.4521-0.22030.61420.25260.57860.4057-0.3351.142764.57819.7940.083
422.3594-2.77382.01473.5782-2.4631.77950.84170.1623-0.42340.34180.33360.68670.2809-0.1950.04261.6411-0.21010.32882.01-0.37831.1133-1.2946.23748.522
431.0009-0.47660.38740.42740.03880.31880.6767-0.4190.2895-0.03690.37170.07030.52270.00611.16420.7776-0.2837-0.08922.4277-0.360.35370.06618.35252.79
441.979-0.1890.80821.537-0.31493.9763-0.0965-0.69970.1042-0.0758-0.22980.3340.43740.22050.12431.36160.02970.11321.5350.00170.7996-11.114.31845.81
454.1906-1.35912.62660.59-1.39364.1333-0.0958-0.06730.07960.33390.25010.2691-0.788-0.01010.12811.46340.3678-0.11641.78520.02730.7866-12.96313.44823.049
460.4330.68210.56242.9260.43370.8113-0.53391.1229-1.16530.4218-0.62980.91-0.0553-0.42060.16991.53990.2615-0.20911.34180.16221.4202-16.60820.77831.84
470.0275-0.05580.02861.7679-1.39581.6326-0.54290.52691.6484-0.15091.3211-0.1407-0.1352-0.23190.00611.90260.27150.23321.82850.0711.0211-16.57125.33738.864
483.5714-0.2597-0.40581.13080.19122.7675-0.6215-0.5159-0.8377-0.2348-0.09320.8403-0.7478-1.91370.00231.21180.169-0.00811.8970.00570.57144.00449.18921.739
490.2965-0.14230.5196-0.0189-0.14790.76040.798-0.9451-1.6735-0.6690.9772-0.28830.2626-1.64320.17191.6603-0.26140.01632.88430.0981.168-3.8442.36836.014
503.7368-4.53531.45535.4894-2.20218.58572.0998-0.6538-0.5245-0.67621.3341-2.80720.6201-02.34751.9205-0.41690.04050.7028-0.17151.35554.88.318-7.629
510.717-0.4877-1.35550.6658-0.24532.6387-0.304-0.5528-0.86240.22410.28910.70480.9708-0.60840.08651.0987-0.0972-0.00190.8103-0.07860.74639.25611.21812.276
521.9960.01230.51741.6661-0.22914.6806-0.3008-0.722-0.5310.54460.3810.88920.4264-2.78730.04791.3022-0.15410.10361.47880.02030.916834.6678.77441.699
533.99221.65560.44021.9690.57985.25980.5667-0.1526-1.2527-0.98141.47712.19830.9511-1.71290.07341.6718-0.00790.18332.71920.16241.5169-8.93419.0685.053
540.0253-0.16170.2671.536-2.05994.6121-0.35740.0044-0.97170.8611-0.7548-1.75880.00190.94040.27982.01270.29280.03262.20090.2671.0191-0.81711.51771.678
553.7893.81750.94873.85360.96740.2299-0.1846-0.3412-2.65120.01740.4238-2.02590.90750.4884-0.28413.0067-0.25340.08223.2410.00371.309315.69613.47858.167
566.82540.18551.06224.3255-0.56036.01060.0105-0.9761-0.15431.9690.7007-0.30521.98640.3555-0.03171.98370.2915-0.23992.8825-0.03731.124810.5016.78143.668
572.0132-0.04810.16070.8148-1.18811.7581-0.76611.0213-0.8988-0.6661-0.9496-1.14490.13020.3847-0.12141.758-0.34910.13793.95460.04841.305111.27111.24225.256
581.6044-1.39860.86581.322-0.08643.50130.38960.3868-0.39470.53770.53510.3711-0.7071-0.98660.13971.5602-0.0331-0.14861.49810.18320.95149.49948.173-7.726
591.2401-0.67060.23822.750.73485.49150.1702-0.79070.33850.32220.817-0.5563-0.40712.1155-0.08341.5752-0.061-0.18991.4885-0.26281.012924.07954.64128.986
605.2232-0.71221.32233.2123-1.96555.116-0.4925-1.09430.060.61890.71380.48071.1712-1.8334-0.01591.6062-0.08580.19891.2048-0.04080.871337.2177.12347.813
613.4175-0.232-0.16941.8644-1.31351.5112-0.25081.27670.48850.16580.43160.78740.82-1.22110.06981.0626-0.28420.02381.11550.04210.779935.5348.79322.709
620.9030.7288-0.11852.1591-0.78210.8064-0.4447-0.6446-0.1387-0.88880.4565-0.15431.3035-0.78880.14311.5385-0.1114-0.09080.86290.02380.554848.4328.570.743
631.76670.58320.50330.75131.17072.04641.23730.9548-0.84940.3578-0.42810.07431.41890.33130.09511.39340.0139-0.15323.1877-0.16421.1859.9519.37332.754
641.3791-1.14982.74721.8615-3.72729.2245-0.0858-1.3673-0.05891.98480.4022-1.3329-0.19490.28110.07212.37350.95650.0333.05590.151.64969.0548.05658.644
653.99421.2604-0.24452.7573-0.09390.00481.4443-0.43740.77250.19090.1120.96791.0941.1050.2362.62810.26810.1812.564-0.09150.5333-2.76215.00976.287
661.3597-0.5668-1.0950.17850.84223.56030.256-0.35950.5878-0.36510.4486-0.2986-1.80720.4267-0.02042.1532-0.0890.21021.3382-0.12730.951220.96955.68330.875
672.1351-0.36210.1751.03740.13711.1331.24672.9417-0.84660.0912-0.00990.0416-0.0862-0.10070.03411.82280.0582-0.05012.00230.06981.013311.58149.696-6.011
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 304:318 )A304 - 318
2X-RAY DIFFRACTION2( CHAIN A AND RESID 319:328 )A319 - 328
3X-RAY DIFFRACTION3( CHAIN A AND RESID 329:337 )A329 - 337
4X-RAY DIFFRACTION4( CHAIN A AND RESID 338:345 )A338 - 345
5X-RAY DIFFRACTION5( CHAIN B AND RESID 29:44 )B29 - 44
6X-RAY DIFFRACTION6( CHAIN B AND RESID 45:52 )B45 - 52
7X-RAY DIFFRACTION7( CHAIN B AND RESID 53:80 )B53 - 80
8X-RAY DIFFRACTION8( CHAIN B AND RESID 81:87 )B81 - 87
9X-RAY DIFFRACTION9( CHAIN B AND RESID 88:100 )B88 - 100
10X-RAY DIFFRACTION10( CHAIN B AND RESID 101:113 )B101 - 113
11X-RAY DIFFRACTION11( CHAIN A AND RESID 66:90 )A66 - 90
12X-RAY DIFFRACTION12( CHAIN A AND RESID 91:125 )A91 - 125
13X-RAY DIFFRACTION13( CHAIN A AND RESID 126:148 )A126 - 148
14X-RAY DIFFRACTION14( CHAIN D AND RESID 1:11 )D1 - 11
15X-RAY DIFFRACTION15( CHAIN D AND RESID 12:25 )D12 - 25
16X-RAY DIFFRACTION16( CHAIN E AND RESID 1:15 )E1 - 15
17X-RAY DIFFRACTION17( CHAIN E AND RESID 16:25 )E16 - 25
18X-RAY DIFFRACTION18( CHAIN F AND RESID 304:318 )F304 - 318
19X-RAY DIFFRACTION19( CHAIN F AND RESID 319:345 )F319 - 345
20X-RAY DIFFRACTION20( CHAIN K AND RESID 304:318 )K304 - 318
21X-RAY DIFFRACTION21( CHAIN K AND RESID 319:328 )K319 - 328
22X-RAY DIFFRACTION22( CHAIN K AND RESID 329:339 )K329 - 339
23X-RAY DIFFRACTION23( CHAIN P AND RESID 304:318 )P304 - 318
24X-RAY DIFFRACTION24( CHAIN P AND RESID 319:345 )P319 - 345
25X-RAY DIFFRACTION25( CHAIN G AND RESID 29:44 )G29 - 44
26X-RAY DIFFRACTION26( CHAIN G AND RESID 45:52 )G45 - 52
27X-RAY DIFFRACTION27( CHAIN G AND RESID 53:80 )G53 - 80
28X-RAY DIFFRACTION28( CHAIN G AND RESID 81:87 )G81 - 87
29X-RAY DIFFRACTION29( CHAIN G AND RESID 88:100 )G88 - 100
30X-RAY DIFFRACTION30( CHAIN G AND RESID 101:114 )G101 - 114
31X-RAY DIFFRACTION31( CHAIN L AND RESID 29:80 )L29 - 80
32X-RAY DIFFRACTION32( CHAIN L AND RESID 81:114 )L81 - 114
33X-RAY DIFFRACTION33( CHAIN Q AND RESID 29:52 )Q29 - 52
34X-RAY DIFFRACTION34( CHAIN Q AND RESID 53:87 )Q53 - 87
35X-RAY DIFFRACTION35( CHAIN Q AND RESID 88:100 )Q88 - 100
36X-RAY DIFFRACTION36( CHAIN Q AND RESID 101:114 )Q101 - 114
37X-RAY DIFFRACTION37( CHAIN F AND RESID 66:81 )F66 - 81
38X-RAY DIFFRACTION38( CHAIN F AND RESID 82:90 )F82 - 90
39X-RAY DIFFRACTION39( CHAIN F AND RESID 91:125 )F91 - 125
40X-RAY DIFFRACTION40( CHAIN F AND RESID 126:142 )F126 - 142
41X-RAY DIFFRACTION41( CHAIN F AND RESID 143:148 )F143 - 148
42X-RAY DIFFRACTION42( CHAIN K AND RESID 68:72 )K68 - 72
43X-RAY DIFFRACTION43( CHAIN K AND RESID 73:82 )K73 - 82
44X-RAY DIFFRACTION44( CHAIN K AND RESID 83:118 )K83 - 118
45X-RAY DIFFRACTION45( CHAIN K AND RESID 119:125 )K119 - 125
46X-RAY DIFFRACTION46( CHAIN K AND RESID 126:135 )K126 - 135
47X-RAY DIFFRACTION47( CHAIN K AND RESID 136:148 )K136 - 148
48X-RAY DIFFRACTION48( CHAIN P AND RESID 69:118 )P69 - 118
49X-RAY DIFFRACTION49( CHAIN P AND RESID 119:147 )P119 - 147
50X-RAY DIFFRACTION50( CHAIN I AND RESID 1:6 )I1 - 6
51X-RAY DIFFRACTION51( CHAIN I AND RESID 7:14 )I7 - 14
52X-RAY DIFFRACTION52( CHAIN I AND RESID 15:25 )I15 - 25
53X-RAY DIFFRACTION53( CHAIN N AND RESID 1:5 )N1 - 5
54X-RAY DIFFRACTION54( CHAIN N AND RESID 6:10 )N6 - 10
55X-RAY DIFFRACTION55( CHAIN N AND RESID 11:15 )N11 - 15
56X-RAY DIFFRACTION56( CHAIN N AND RESID 16:20 )N16 - 20
57X-RAY DIFFRACTION57( CHAIN N AND RESID 21:25 )N21 - 25
58X-RAY DIFFRACTION58( CHAIN T AND RESID 1:11 )T1 - 11
59X-RAY DIFFRACTION59( CHAIN T AND RESID 12:25 )T12 - 25
60X-RAY DIFFRACTION60( CHAIN J AND RESID 1:9 )J1 - 9
61X-RAY DIFFRACTION61( CHAIN J AND RESID 10:16 )J10 - 16
62X-RAY DIFFRACTION62( CHAIN J AND RESID 17:25 )J17 - 25
63X-RAY DIFFRACTION63( CHAIN O AND RESID 1:11 )O1 - 11
64X-RAY DIFFRACTION64( CHAIN O AND RESID 12:16 )O12 - 16
65X-RAY DIFFRACTION65( CHAIN O AND RESID 17:25 )O17 - 25
66X-RAY DIFFRACTION66( CHAIN U AND RESID 1:15 )U1 - 15
67X-RAY DIFFRACTION67( CHAIN U AND RESID 16:25 )U16 - 25

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