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- PDB-7cpc: His-Mediated Reversible Self-assembly of Ferritin Nanocage with N... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7cpc | |||||||||
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Title | His-Mediated Reversible Self-assembly of Ferritin Nanocage with Ni binding | |||||||||
![]() | Ferritin | |||||||||
![]() | METAL BINDING PROTEIN / Ferritin / Assembly / Ni binding | |||||||||
Function / homology | ![]() ferroxidase / intracellular sequestering of iron ion / ferroxidase activity / ferric iron binding / ferrous iron binding / iron ion transport / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Gu, C. / Zhang, T. / Zhao, G. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: His-Mediated Reversible Self-Assembly of Ferritin Nanocages through Two Different Switches for Encapsulation of Cargo Molecules. Authors: Gu, C. / Zhang, T. / Lv, C. / Liu, Y. / Wang, Y. / Zhao, G. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 206.5 KB | Display | ![]() |
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PDB format | ![]() | 166.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7cpiC ![]() 6kh1S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 19735.049 Da / Num. of mol.: 6 / Mutation: Q89R Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-NI / #3: Chemical | ChemComp-FE / #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.95 Å3/Da / Density % sol: 58.29 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion / Details: 1.26 M (NH4)2SO4, 100 mM Tris (pH =8.0) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jan 10, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97892 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→50 Å / Num. obs: 33438 / % possible obs: 99.3 % / Redundancy: 4.6 % / CC1/2: 0.959 / Net I/σ(I): 7.545 |
Reflection shell | Resolution: 2.8→2.85 Å / Num. unique obs: 1668 / CC1/2: 0.789 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6KH1 Resolution: 2.801→36.275 Å / SU ML: 0.42 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 27.22 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 89.66 Å2 / Biso mean: 27.4982 Å2 / Biso min: 7.96 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.801→36.275 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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