- PDB-7cnf: Solution strcture of HsTFIIS LW domain -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 7cnf
Title
Solution strcture of HsTFIIS LW domain
Components
Transcription elongation factor A protein 1
Keywords
PEPTIDE BINDING PROTEIN / binding prorein
Function / homology
Function and homology information
Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / transcription factor TFIID complex / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / Formation of HIV elongation complex in the absence of HIV Tat / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex ...Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / transcription factor TFIID complex / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / Formation of HIV elongation complex in the absence of HIV Tat / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / RNA Polymerase II Pre-transcription Events / transcription elongation by RNA polymerase II / TP53 Regulates Transcription of DNA Repair Genes / Transcription-Coupled Nucleotide Excision Repair (TC-NER) / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / transcription by RNA polymerase II / DNA-templated transcription / nucleolus / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleoplasm / nucleus Similarity search - Function
Transcription elongation factor, TFIIS / Transcription elongation factor, IIS-type / Transcription factor S-II (TFIIS), central domain / Domain in the central regions of transcription elongation factor S-II (and elsewhere) / Transcription elongation factor S-II, central domain / Transcription elongation factor S-II, central domain superfamily / TFIIS central domain profile. / Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type / Domain in the N-terminus of transcription elongation factor S-II (and elsewhere) / TFIIS helical bundle-like domain ...Transcription elongation factor, TFIIS / Transcription elongation factor, IIS-type / Transcription factor S-II (TFIIS), central domain / Domain in the central regions of transcription elongation factor S-II (and elsewhere) / Transcription elongation factor S-II, central domain / Transcription elongation factor S-II, central domain superfamily / TFIIS central domain profile. / Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type / Domain in the N-terminus of transcription elongation factor S-II (and elsewhere) / TFIIS helical bundle-like domain / Transcription factor IIS, N-terminal / TFIIS N-terminal domain profile. / TFIIS/LEDGF domain superfamily / Zinc finger TFIIS-type signature. / Zinc finger, TFIIS-type / Transcription factor S-II (TFIIS) / Zinc finger TFIIS-type profile. / C2C2 Zinc finger Similarity search - Domain/homology
Mass: 10835.714 Da / Num. of mol.: 1 / Fragment: LW domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TCEA1, GTF2S, TFIIS / Production host: Escherichia phage EcSzw-2 (virus) / References: UniProt: P23193
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
2D 1H-15N HSQC NH2 only
1
2
1
isotropic
1
3DCBCA(CO)NH
1
3
1
isotropic
1
3D CBCANH
1
4
1
isotropic
1
3DHBHA(CO)NH
1
5
1
isotropic
1
15N-edited NOESY
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Sample preparation
Details
Type: solution Contents: 2.5 g/L [U-95% 13C] glucose, 0.5 g/L [U-15N] Ammonium Chloride, 25 mM NA sodium phosphate, 2 mM NA EDTA, 90% H2O/10% D2O Label: 13C_15N_HsLW / Solvent system: 90% H2O/10% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
2.5g/L
glucose
[U-95% 13C]
1
0.5g/L
AmmoniumChloride
[U-15N]
1
25mM
sodiumphosphate
NA
1
2mM
EDTA
NA
1
Sample conditions
Ionic strength: 175 mM / Label: sodium phosphate / pH: 6.8 / Pressure: 1 Pa / Temperature: 298 K
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NMR measurement
NMR spectrometer
Type: Bruker DMX 600 / Manufacturer: Bruker / Model: DMX 600 / Field strength: 600 MHz
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Processing
NMR software
Name
Developer
Classification
CYANA
Guntert, MumenthalerandWuthrich
structurecalculation
NMRDraw
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
dataanalysis
NMRPipe
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
chemicalshiftassignment
Sparky
Goddard
peakpicking
Refinement
Method: simulated annealing / Software ordinal: 1
NMR representative
Selection criteria: fewest violations
NMR ensemble
Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 200 / Conformers submitted total number: 20
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