+Open data
-Basic information
Entry | Database: PDB / ID: 7cdv | ||||||
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Title | STRUCTURE OF A NOVEL VIRULENCE REGULATION FACTOR SghR | ||||||
Components | LacI-type transcription factor | ||||||
Keywords | TRANSCRIPTION / AGROBACTERIUM INFECTION / PLANT-MICROBE INTERACTION / LACI REP SUCROSE | ||||||
Function / homology | Function and homology information transcription cis-regulatory region binding / DNA-binding transcription factor activity Similarity search - Function | ||||||
Biological species | Agrobacterium tumefaciens A6 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.1 Å | ||||||
Authors | Ye, F.Z. / Wang, C. / Yan, X.F. / Zhang, L.H. / Gao, Y.G. | ||||||
Funding support | Singapore, 1items
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Citation | Journal: J.Biol.Chem. / Year: 2020 Title: Structural basis of a novel repressor, SghR, controllingAgrobacteriuminfection by cross-talking to plants. Authors: Ye, F. / Wang, C. / Fu, Q. / Yan, X.F. / Bharath, S.R. / Casanas, A. / Wang, M. / Song, H. / Zhang, L.H. / Gao, Y.G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7cdv.cif.gz | 239.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7cdv.ent.gz | 189.8 KB | Display | PDB format |
PDBx/mmJSON format | 7cdv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7cdv_validation.pdf.gz | 444.4 KB | Display | wwPDB validaton report |
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Full document | 7cdv_full_validation.pdf.gz | 448.1 KB | Display | |
Data in XML | 7cdv_validation.xml.gz | 25.1 KB | Display | |
Data in CIF | 7cdv_validation.cif.gz | 35.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cd/7cdv ftp://data.pdbj.org/pub/pdb/validation_reports/cd/7cdv | HTTPS FTP |
-Related structure data
Related structure data | 7cdxC 7ce1C 3gv0S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 40776.398 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Agrobacterium tumefaciens A6 (bacteria) Gene: sghR / Plasmid: PET14B-SGHR / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 CODONPLUS-(DE3) RIL / References: UniProt: A0A2I4PGE9 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.59 Å3/Da / Density % sol: 22.45 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.05M HEPES-NA PH 7.0, 20% PEG 3350, 1%(W/V) TRYPTONE, VAPOR DIFFUSION, TEMPERATURE 293K |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Dec 21, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→50 Å / Num. obs: 31202 / % possible obs: 99.7 % / Redundancy: 3.5 % / Rsym value: 0.08 / Net I/σ(I): 9.6 |
Reflection shell | Resolution: 2.1→2.21 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.33 / Mean I/σ(I) obs: 3.7 / Num. unique obs: 31202 / % possible all: 99.9 |
-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3GV0 Resolution: 2.1→42.66 Å / SU ML: 0.23 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 28.34 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 97.6 Å2 / Biso mean: 26.854 Å2 / Biso min: 6.45 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.1→42.66 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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Refinement TLS params. | Method: refined / Origin x: 0.0415 Å / Origin y: 1.6577 Å / Origin z: 26.9851 Å
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Refinement TLS group |
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