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Yorodumi- PDB-7c3l: Structure of L-lysine oxidase D212A/D315A in complex with L-tyrosine -
+Open data
-Basic information
Entry | Database: PDB / ID: 7c3l | ||||||
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Title | Structure of L-lysine oxidase D212A/D315A in complex with L-tyrosine | ||||||
Components | L-lysine oxidase | ||||||
Keywords | OXIDOREDUCTASE / L-amino acid oxidase | ||||||
Function / homology | Function and homology information L-lysine oxidase / L-lysine oxidase activity / L-amino-acid oxidase activity / amino acid catabolic process / nucleotide binding Similarity search - Function | ||||||
Biological species | Hypocrea rufa (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.8 Å | ||||||
Authors | Kitagawa, M. / Matsumoto, Y. / Inagaki, K. / Imada, K. | ||||||
Funding support | Japan, 1items
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Citation | Journal: Protein Sci. / Year: 2020 Title: Structural basis of strict substrate recognition of l-lysine alpha-oxidase from Trichoderma viride. Authors: Kondo, H. / Kitagawa, M. / Matsumoto, Y. / Saito, M. / Amano, M. / Sugiyama, S. / Tamura, T. / Kusakabe, H. / Inagaki, K. / Imada, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7c3l.cif.gz | 273.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7c3l.ent.gz | 197.1 KB | Display | PDB format |
PDBx/mmJSON format | 7c3l.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7c3l_validation.pdf.gz | 1.4 MB | Display | wwPDB validaton report |
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Full document | 7c3l_full_validation.pdf.gz | 1.4 MB | Display | |
Data in XML | 7c3l_validation.xml.gz | 51.7 KB | Display | |
Data in CIF | 7c3l_validation.cif.gz | 78.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c3/7c3l ftp://data.pdbj.org/pub/pdb/validation_reports/c3/7c3l | HTTPS FTP |
-Related structure data
Related structure data | 7c3hC 7c3iC 7c3jC 3x0vS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 60787.598 Da / Num. of mol.: 2 / Mutation: D212A,D315A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Hypocrea rufa (fungus) / Plasmid: pCold IV / Production host: Escherichia coli (E. coli) / Strain (production host): SoluBL21 References: UniProt: A0A0J9X1X3, UniProt: A0A0G4DCU0*PLUS, L-lysine oxidase |
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-Non-polymers , 5 types, 1357 molecules
#2: Chemical | #3: Chemical | #4: Chemical | #5: Chemical | ChemComp-GOL / #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.24 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 1% (w/v) PEG400, 0.1 M Tris-HCl pH7.5, 2.1 M Ammonium Sulfate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 1 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 14, 2018 |
Radiation | Monochromator: Double-crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→75.3 Å / Num. obs: 111001 / % possible obs: 99.8 % / Redundancy: 5.8 % / Biso Wilson estimate: 17.03 Å2 / Rmerge(I) obs: 0.088 / Net I/σ(I): 9.9 |
Reflection shell | Resolution: 1.8→1.83 Å / Redundancy: 6.2 % / Rmerge(I) obs: 0.235 / Mean I/σ(I) obs: 5 / Num. unique obs: 5429 / % possible all: 99.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3X0V Resolution: 1.8→69.58 Å / SU ML: 0.1711 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 23.8207 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.4 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→69.58 Å
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Refine LS restraints |
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LS refinement shell |
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