+Open data
-Basic information
Entry | Database: PDB / ID: 7c0g | ||||||
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Title | Aca1 in complex with 14bp palindromic DNA target | ||||||
Components |
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Keywords | TRANSCRIPTION / CRISPR / anti-CRISPR / anti-CRISPR-associated / DNA binding / autoregulation / Aca1 / complex | ||||||
Function / homology | Lambda repressor-like, DNA-binding domain superfamily / DNA binding / DNA / DNA (> 10) / HTH cro/C1-type domain-containing protein Function and homology information | ||||||
Biological species | Pseudomonas phage JBD30 (virus) synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Liu, Y.H. / Zhang, L.S. / Wu, B.X. / Huang, H.D. | ||||||
Citation | Journal: To Be Published Title: Aca1 in complex with 14bp palindromic DNA target Authors: Liu, Y.H. / Zhang, L.S. / Wu, B.X. / Huang, H.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7c0g.cif.gz | 58.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7c0g.ent.gz | 40.8 KB | Display | PDB format |
PDBx/mmJSON format | 7c0g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7c0g_validation.pdf.gz | 445.2 KB | Display | wwPDB validaton report |
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Full document | 7c0g_full_validation.pdf.gz | 448.5 KB | Display | |
Data in XML | 7c0g_validation.xml.gz | 9.4 KB | Display | |
Data in CIF | 7c0g_validation.cif.gz | 12.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c0/7c0g ftp://data.pdbj.org/pub/pdb/validation_reports/c0/7c0g | HTTPS FTP |
-Related structure data
Related structure data | 7c0b 7c0a S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 8908.148 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas phage JBD30 (virus) / Gene: JBD30_036 / Production host: Escherichia coli (E. coli) / References: UniProt: L7P845 #2: DNA chain | Mass: 4281.779 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.57 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.1M Sodium acetate pH5.0; 20%(w/v) MPD |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.9789 Å |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Oct 15, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9789 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→40 Å / Num. obs: 12045 / % possible obs: 99.5 % / Redundancy: 27.4 % / CC1/2: 1 / CC star: 1 / Rmerge(I) obs: 0.133 / Rsym value: 0.133 / Net I/σ(I): 24.333 |
Reflection shell | Resolution: 2.4→2.49 Å / Rmerge(I) obs: 0.814 / Num. unique obs: 1108 / CC1/2: 0.385 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 7C0B 7c0b Resolution: 2.4→35 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso mean: 31.69 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→35 Å
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Refine LS restraints |
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