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Open data
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Basic information
Entry | Database: PDB / ID: 7c0g | ||||||
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Title | Aca1 in complex with 14bp palindromic DNA target | ||||||
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![]() | TRANSCRIPTION / CRISPR / anti-CRISPR / anti-CRISPR-associated / DNA binding / autoregulation / Aca1 / complex | ||||||
Function / homology | Lambda repressor-like, DNA-binding domain superfamily / DNA binding / DNA / DNA (> 10) / HTH cro/C1-type domain-containing protein![]() | ||||||
Biological species | ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liu, Y.H. / Zhang, L.S. / Wu, B.X. / Huang, H.D. | ||||||
![]() | ![]() Title: Aca1 in complex with 14bp palindromic DNA target Authors: Liu, Y.H. / Zhang, L.S. / Wu, B.X. / Huang, H.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 58.7 KB | Display | ![]() |
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PDB format | ![]() | 40.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 445.2 KB | Display | ![]() |
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Full document | ![]() | 448.5 KB | Display | |
Data in XML | ![]() | 9.4 KB | Display | |
Data in CIF | ![]() | 12.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7c0b ![]() 7c0a S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 8908.148 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: DNA chain | Mass: 4281.779 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.57 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.1M Sodium acetate pH5.0; 20%(w/v) MPD |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Oct 15, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9789 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→40 Å / Num. obs: 12045 / % possible obs: 99.5 % / Redundancy: 27.4 % / CC1/2: 1 / CC star: 1 / Rmerge(I) obs: 0.133 / Rsym value: 0.133 / Net I/σ(I): 24.333 |
Reflection shell | Resolution: 2.4→2.49 Å / Rmerge(I) obs: 0.814 / Num. unique obs: 1108 / CC1/2: 0.385 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 7C0B ![]() 7c0b Resolution: 2.4→35 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso mean: 31.69 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→35 Å
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Refine LS restraints |
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