+Open data
-Basic information
Entry | Database: PDB / ID: 7bz7 | ||||||||||||
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Title | Template lasso peptide C24 mutant F15Y | ||||||||||||
Components | lasso peptide | ||||||||||||
Keywords | ANTIBIOTIC / heterologous expression / site-direct mutagenesis | ||||||||||||
Biological species | Streptomyces sp. (bacteria) | ||||||||||||
Method | SOLUTION NMR / simulated annealing | ||||||||||||
Authors | Liu, X.H. / Liu, T. / Ma, X.J. / Yu, J.H. / Yang, D.H. / Ma, M. | ||||||||||||
Funding support | China, 3items
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Citation | Journal: Chem Sci / Year: 2021 Title: Rational generation of lasso peptides based on biosynthetic gene mutations and site-selective chemical modifications. Authors: Liu, T. / Ma, X. / Yu, J. / Yang, W. / Wang, G. / Wang, Z. / Ge, Y. / Song, J. / Han, H. / Zhang, W. / Yang, D. / Liu, X. / Ma, M. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7bz7.cif.gz | 85.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7bz7.ent.gz | 57.6 KB | Display | PDB format |
PDBx/mmJSON format | 7bz7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7bz7_validation.pdf.gz | 431.9 KB | Display | wwPDB validaton report |
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Full document | 7bz7_full_validation.pdf.gz | 536.7 KB | Display | |
Data in XML | 7bz7_validation.xml.gz | 11.2 KB | Display | |
Data in CIF | 7bz7_validation.cif.gz | 14.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bz/7bz7 ftp://data.pdbj.org/pub/pdb/validation_reports/bz/7bz7 | HTTPS FTP |
-Related structure data
Related structure data | 6m19C 7bz8C 7bz9C 7bzaC 7cu6C C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1730.958 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces sp. (bacteria) / Production host: Streptomyces sp. (bacteria) |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 0.83 mM C24_F15Y, 0.2 v/v DMSO, 100 mM NaCl, 0.8 v/v H2O, 20% DMSO/80% H2O Label: f15y / Solvent system: 20% DMSO/80% H2O | ||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 100 mM / Label: condition1 / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Agilent DD2 / Manufacturer: Agilent / Model: DD2 / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 4 | ||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |