[English] 日本語
Yorodumi- PDB-1m2c: THREE-DIMENSIONAL STRUCTURE OF ALPHA-CONOTOXIN MII, NMR, 14 STRUCTURES -
+Open data
-Basic information
Entry | Database: PDB / ID: 1m2c | ||||||
---|---|---|---|---|---|---|---|
Title | THREE-DIMENSIONAL STRUCTURE OF ALPHA-CONOTOXIN MII, NMR, 14 STRUCTURES | ||||||
Components | ALPHA-CONOTOXIN MII | ||||||
Keywords | NEUROTOXIN / NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR INHIBITOR / SUBTYPE SPECIFIC LIGAND / PRESYNAPTIC NICOTINIC ACETYLCHOLINE RECEPTOR BLOCKER / CHOLINERGIC MODULATION / DOPAMINE RELEASE | ||||||
Function / homology | Function and homology information host cell postsynaptic membrane / acetylcholine receptor inhibitor activity / ion channel regulator activity / toxin activity / extracellular region Similarity search - Function | ||||||
Biological species | Conus magus (magus cone) | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, ITERATIVE RELAXATION MATRIX APPROACH, SIMULATED ANNEALING | ||||||
Authors | Shon, K.J. / Koerber, S.C. / Rivier, J.E. / Olivera, B.M. / Mcintosh, J.M. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1996 Title: A new alpha-conotoxin which targets alpha3beta2 nicotinic acetylcholine receptors. Authors: Cartier, G.E. / Yoshikami, D. / Gray, W.R. / Luo, S. / Olivera, B.M. / McIntosh, J.M. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1m2c.cif.gz | 59.3 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1m2c.ent.gz | 44.8 KB | Display | PDB format |
PDBx/mmJSON format | 1m2c.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m2/1m2c ftp://data.pdbj.org/pub/pdb/validation_reports/m2/1m2c | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein/peptide | Mass: 1715.976 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Conus magus (magus cone) / References: UniProt: P56636 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||||||
NMR details | Text: THE STRUCTURE WAS DETERMINED USING 2-D HOMONUCLEAR 1H NMR EXPERIMENTS. THESE ARE A SET OF NOESY, TOCSY, DQF-COSY, AND PE-COSY. |
-Sample preparation
Details | Contents: 90% H2O / 10% D2O |
---|---|
Sample conditions | Ionic strength: 5mM SODIUM PHOSPHATE BUFFER / pH: 3.3 / Pressure: 1 atm / Temperature: 275 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITYPLUS / Manufacturer: Varian / Model: UNITYPLUS / Field strength: 600 MHz |
---|
-Processing
NMR software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: DISTANCE GEOMETRY, ITERATIVE RELAXATION MATRIX APPROACH, SIMULATED ANNEALING Software ordinal: 1 | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY / Conformers calculated total number: 50 / Conformers submitted total number: 14 |