[English] 日本語
![](img/lk-miru.gif)
- PDB-7b3o: Crystal structure of the SARS-CoV-2 RBD in complex with STE90-C11 Fab -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 7b3o | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of the SARS-CoV-2 RBD in complex with STE90-C11 Fab | ||||||
![]() |
| ||||||
![]() | ANTIVIRAL PROTEIN / neutralizing antibodies / SARS-CoV-2 / RBD | ||||||
Function / homology | ![]() Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / receptor-mediated endocytosis of virus by host cell / membrane fusion / Attachment and Entry / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / identical protein binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Kluenemann, T. / Van den Heuvel, J. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations. Authors: Bertoglio, F. / Fuhner, V. / Ruschig, M. / Heine, P.A. / Abassi, L. / Klunemann, T. / Rand, U. / Meier, D. / Langreder, N. / Steinke, S. / Ballmann, R. / Schneider, K.T. / Roth, K.D.R. / ...Authors: Bertoglio, F. / Fuhner, V. / Ruschig, M. / Heine, P.A. / Abassi, L. / Klunemann, T. / Rand, U. / Meier, D. / Langreder, N. / Steinke, S. / Ballmann, R. / Schneider, K.T. / Roth, K.D.R. / Kuhn, P. / Riese, P. / Schackermann, D. / Korn, J. / Koch, A. / Chaudhry, M.Z. / Eschke, K. / Kim, Y. / Zock-Emmenthal, S. / Becker, M. / Scholz, M. / Moreira, G.M.S.G. / Wenzel, E.V. / Russo, G. / Garritsen, H.S.P. / Casu, S. / Gerstner, A. / Roth, G. / Adler, J. / Trimpert, J. / Hermann, A. / Schirrmann, T. / Dubel, S. / Frenzel, A. / Van den Heuvel, J. / Cicin-Sain, L. / Schubert, M. / Hust, M. #1: ![]() Title: A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations. Authors: Bertoglio, F. / Fuhner, V. / Ruschig, M. / Heine, P.A. / Abassi, L. / Klunemann, T. / Rand, U. / Meier, D. / Langreder, N. / Steinke, S. / Ballmann, R. / Schneider, K.T. / Roth, K.D.R. / ...Authors: Bertoglio, F. / Fuhner, V. / Ruschig, M. / Heine, P.A. / Abassi, L. / Klunemann, T. / Rand, U. / Meier, D. / Langreder, N. / Steinke, S. / Ballmann, R. / Schneider, K.T. / Roth, K.D.R. / Kuhn, P. / Riese, P. / Schackermann, D. / Korn, J. / Koch, A. / Chaudhry, M.Z. / Eschke, K. / Kim, Y. / Zock-Emmenthal, S. / Becker, M. / Scholz, M. / Moreira, G.M.S.G. / Wenzel, E.V. / Russo, G. / Garritsen, H.S.P. / Casu, S. / Gerstner, A. / Roth, G. / Adler, J. / Trimpert, J. / Hermann, A. / Schirrmann, T. / Dubel, S. / Frenzel, A. / Van den Heuvel, J. / Cicin-Sain, L. / Schubert, M. / Hust, M. #2: ![]() Title: A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients by phage display is binding to the ACE2-RBD interface and is tolerant to known RBD mutations Authors: Bertoglio, F. / Fuhner, V. / Ruschig, M. / Heine, P.A. / Rand, U. / Klunemann, T. / Meier, D. / Langreder, N. / Steinke, S. / Ballmann, R. / Schneider, K. / Roth, K.D.R. / Kuhn, P. / Riese, ...Authors: Bertoglio, F. / Fuhner, V. / Ruschig, M. / Heine, P.A. / Rand, U. / Klunemann, T. / Meier, D. / Langreder, N. / Steinke, S. / Ballmann, R. / Schneider, K. / Roth, K.D.R. / Kuhn, P. / Riese, P. / Schackermann, D. / Korn, J. / Koch, A. / Zock-Emmenthal, S. / Becker, M. / Scholz, M. / Moreira, G.M.S.G. / Wenzel, E.V. / Russo, G. / Garritsen, H.S. / Casu, S. / Gerstner, A. / Roth, G. / Hermann, A. / Schirrmann, T. / Dubel, S. / Frenzel, A. / Cicin-Sain, L. / Schubert, M. / Hust, M. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 243.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 195 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 607.8 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 611 KB | Display | |
Data in XML | ![]() | 27 KB | Display | |
Data in CIF | ![]() | 40.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7bwyS S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 23087.838 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: S, 2 / Production host: ![]() |
---|---|
#2: Antibody | Mass: 23481.197 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#3: Antibody | Mass: 24081.908 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#4: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
#5: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3.4 Å3/Da / Density % sol: 63.78 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion Details: 13.3% (w/v) polyethylene glycol 6,000, 0.1M MES pH 5.6 0.24M tri sodium citrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Sep 4, 2020 | ||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1.033 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 2→48.1 Å / Num. obs: 63906 / % possible obs: 100 % / Redundancy: 6.9 % / CC1/2: 0.985 / Rmerge(I) obs: 0.159 / Rpim(I) all: 0.066 / Rrim(I) all: 0.173 / Net I/σ(I): 6.7 / Num. measured all: 437879 / Scaling rejects: 33 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
|
-Phasing
Phasing | Method: ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Phasing MR |
|
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 7bwy Resolution: 2→48.1 Å / SU ML: 0.28 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 26.87 / Stereochemistry target values: ML
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 160.82 Å2 / Biso mean: 47.2921 Å2 / Biso min: 22.59 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2→48.1 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 8 / % reflection obs: 100 %
|