+Open data
-Basic information
Entry | Database: PDB / ID: 4xdn | |||||||||
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Title | Crystal structure of Scc4 in complex with Scc2n | |||||||||
Components |
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Keywords | CELL CYCLE / cohesion / centromere / cohesin loading | |||||||||
Function / homology | Function and homology information SMC loading complex / Scc2-Scc4 cohesin loading complex / mitotic cohesin loading / 2-micrometer circle DNA / tRNA gene clustering / rDNA chromatin condensation / establishment of protein localization to chromatin / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / kinetochore binding / replication-born double-strand break repair via sister chromatid exchange ...SMC loading complex / Scc2-Scc4 cohesin loading complex / mitotic cohesin loading / 2-micrometer circle DNA / tRNA gene clustering / rDNA chromatin condensation / establishment of protein localization to chromatin / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / kinetochore binding / replication-born double-strand break repair via sister chromatid exchange / establishment of mitotic sister chromatid cohesion / mitotic chromosome condensation / mitotic sister chromatid cohesion / chromosome, centromeric region / protein localization to chromatin / double-strand break repair / regulation of gene expression / sequence-specific DNA binding / cell division / chromatin binding / chromatin / nucleus / cytosol Similarity search - Function | |||||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.08 Å | |||||||||
Authors | Hinshaw, S.M. / Harrison, S.C. | |||||||||
Funding support | United States, 2items
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Citation | Journal: Elife / Year: 2015 Title: Structural evidence for Scc4-dependent localization of cohesin loading. Authors: Hinshaw, S.M. / Makrantoni, V. / Kerr, A. / Marston, A.L. / Harrison, S.C. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4xdn.cif.gz | 288.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4xdn.ent.gz | 230.9 KB | Display | PDB format |
PDBx/mmJSON format | 4xdn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xd/4xdn ftp://data.pdbj.org/pub/pdb/validation_reports/xd/4xdn | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 74557.156 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: SCC4 / Production host: Escherichia coli (E. coli) / References: UniProt: P40090 | ||
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#2: Protein | Mass: 21868.424 Da / Num. of mol.: 1 / Fragment: N-terminal region (UNP residues 1-181) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: SCC2 / Production host: Escherichia coli (E. coli) / References: UniProt: Q04002 | ||
#3: Chemical | ChemComp-SO4 / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.84 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, sitting drop Details: overnight growth in .2M ammonium sulfate, 16% (w:v) PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Apr 8, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.079→30 Å / Num. obs: 50794 / % possible obs: 99.9 % / Redundancy: 3.7 % / Rmerge(I) obs: 0.098 / Net I/σ(I): 9.4 |
Reflection shell | Resolution: 2.079→2.14 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.762 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.08→28.72 Å / SU ML: 0.21 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 25.1 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||
Displacement parameters | Biso mean: 44.5 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.08→28.72 Å
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