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Open data
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Basic information
Entry | Database: PDB / ID: 7b22 | ||||||
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Title | Vibrio cholerae ParD2 Antitoxin | ||||||
![]() | Antitoxin ParD | ||||||
![]() | DNA BINDING PROTEIN / Prokaryotic Toxin-Antitoxin System / intrinsically disordered proteins / RHH protein / transcriptional repressor / Antitoxin | ||||||
Function / homology | Antitoxin ParD superfamily / Bacterial antitoxin of ParD toxin-antitoxin type II system and RHH / Antitoxin ParD / Ribbon-helix-helix / regulation of DNA-templated transcription / Antitoxin ParD![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Garcia-Rodriguez, G. / Loris, R. | ||||||
![]() | ![]() Title: Entropic pressure controls the oligomerization of the Vibrio cholerae ParD2 antitoxin. Authors: Garcia-Rodriguez, G. / Girardin, Y. / Volkov, A.N. / Singh, R.K. / Muruganandam, G. / Van Dyck, J. / Sobott, F. / Versees, W. / Charlier, D. / Loris, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 82.9 KB | Display | ![]() |
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PDB format | ![]() | 62.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 472.7 KB | Display | ![]() |
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Full document | ![]() | 473.9 KB | Display | |
Data in XML | ![]() | 13.8 KB | Display | |
Data in CIF | ![]() | 18.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3kxeS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 8972.801 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 39315 / El Tor Inaba N16961 / Gene: parD, VC_A0360.1 / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52.27 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 0.2 M Lithium sulfate, 0.1 M MES pH 6 and 20 % w/v PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Sep 6, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9801 Å / Relative weight: 1 |
Reflection | Resolution: 3.08→45.95 Å / Num. obs: 8845 / % possible obs: 99.5 % / Redundancy: 6.8 % / CC1/2: 0.996 / Rmerge(I) obs: 0.1738 / Net I/σ(I): 7.36 |
Reflection shell | Resolution: 3.08→3.19 Å / Rmerge(I) obs: 1.41 / Num. unique obs: 842 / CC1/2: 0.383 |
-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3kxe Resolution: 3.08→45.95 Å / SU ML: 0.49 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 35.02 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||
Displacement parameters | Biso max: 195.24 Å2 / Biso mean: 84.5736 Å2 / Biso min: 52.83 Å2 | ||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.08→45.95 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 3
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