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- PDB-7ab4: Crystal structure of the Escherichia coli toxin-antitoxin system ... -

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Basic information

Entry
Database: PDB / ID: 7ab4
TitleCrystal structure of the Escherichia coli toxin-antitoxin system HipBST (HipT S59A)
Components
  • (HipA_C domain-containing protein) x 2
  • Couple_hipA domain-containing protein
  • Predicted transcriptional regulator, XRE family
KeywordsTOXIN / antitoxin / kinase / HTH / HipA / HipT / HipB / HipS / HipBA / HipBST / bacteria / TrpS / phosphorylation / trans-autophosphorylation
Function / homology
Function and homology information


transferase activity / DNA binding
Similarity search - Function
: / HipA, N-terminal subdomain 1 / HipA N-terminal domain / HipA-like, C-terminal / HipA-like C-terminal domain / Helix-turn-helix / Cro/C1-type helix-turn-helix domain / Lambda repressor-like, DNA-binding domain superfamily
Similarity search - Domain/homology
HipA-like C-terminal domain-containing protein / HipA N-terminal subdomain 1 domain-containing protein / Predicted transcriptional regulator, XRE family
Similarity search - Component
Biological speciesEscherichia coli O127:H6 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.34 Å
AuthorsBaerentsen, R.L. / Brodersen, D.E.
Funding support Denmark, 2items
OrganizationGrant numberCountry
Novo Nordisk FoundationNNF18OC0030646 Denmark
Danish National Research FoundationDNRF120 Denmark
CitationJournal: Biorxiv / Year: 2022
Title: Structural basis for regulation of a tripartite toxin-antitoxin system by dual phosphorylation
Authors: Baerentsen, R.L. / Nielsen, S.V. / Lyngso, J. / Bisiak, F. / Pedersen, J.S. / Gerdes, K. / Sorensen, M.A. / Brodersen, D.E.
History
DepositionSep 6, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jan 12, 2022Provider: repository / Type: Initial release
Revision 1.1Aug 3, 2022Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.pdbx_database_id_DOI / _citation.title / _citation.year
Revision 1.2May 1, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / citation / pdbx_initial_refinement_model
Item: _citation.journal_id_ISSN

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Predicted transcriptional regulator, XRE family
B: Couple_hipA domain-containing protein
C: HipA_C domain-containing protein
D: Predicted transcriptional regulator, XRE family
E: Couple_hipA domain-containing protein
F: HipA_C domain-containing protein


Theoretical massNumber of molelcules
Total (without water)125,0546
Polymers125,0546
Non-polymers00
Water1448
1


  • Idetical with deposited unit
  • defined by software
  • Evidence: SAXS
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9970 Å2
ΔGint-55 kcal/mol
Surface area45870 Å2
MethodPISA
Unit cell
Length a, b, c (Å)285.257, 107.146, 58.449
Angle α, β, γ (deg.)90.000, 90.710, 90.000
Int Tables number5
Space group name H-MC121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and ((resid 36 through 37 and (name N...
21(chain D and (resid 36 through 77 or resid 79 through 81 or resid 83 through 107))
12(chain B and (resid 2 or resid 5 or resid...
22(chain E and (resid 2 or resid 5 or resid...
13(chain C and (resid 2 through 12 or resid 14...
23(chain F and (resid 2 through 12 or resid 14...

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111(chain A and ((resid 36 through 37 and (name N...A36 - 37
121(chain A and ((resid 36 through 37 and (name N...A36 - 107
131(chain A and ((resid 36 through 37 and (name N...A36 - 107
141(chain A and ((resid 36 through 37 and (name N...A36 - 107
151(chain A and ((resid 36 through 37 and (name N...A36 - 107
211(chain D and (resid 36 through 77 or resid 79 through 81 or resid 83 through 107))D36 - 77
221(chain D and (resid 36 through 77 or resid 79 through 81 or resid 83 through 107))D79 - 81
231(chain D and (resid 36 through 77 or resid 79 through 81 or resid 83 through 107))D83 - 107
112(chain B and (resid 2 or resid 5 or resid...B2
122(chain B and (resid 2 or resid 5 or resid...B5
132(chain B and (resid 2 or resid 5 or resid...B7 - 11
142(chain B and (resid 2 or resid 5 or resid...B13 - 15
152(chain B and (resid 2 or resid 5 or resid...B17
162(chain B and (resid 2 or resid 5 or resid...B69
172(chain B and (resid 2 or resid 5 or resid...B2 - 101
182(chain B and (resid 2 or resid 5 or resid...B2 - 101
192(chain B and (resid 2 or resid 5 or resid...B2 - 101
212(chain E and (resid 2 or resid 5 or resid...E2
222(chain E and (resid 2 or resid 5 or resid...E5
232(chain E and (resid 2 or resid 5 or resid...E7 - 11
242(chain E and (resid 2 or resid 5 or resid...E1
252(chain E and (resid 2 or resid 5 or resid...E80 - 8
262(chain E and (resid 2 or resid 5 or resid...E3
272(chain E and (resid 2 or resid 5 or resid...E85 - 101
113(chain C and (resid 2 through 12 or resid 14...C2 - 12
123(chain C and (resid 2 through 12 or resid 14...C14 - 32
133(chain C and (resid 2 through 12 or resid 14...C34 - 46
143(chain C and (resid 2 through 12 or resid 14...C55 - 56
153(chain C and (resid 2 through 12 or resid 14...C58 - 69
163(chain C and (resid 2 through 12 or resid 14...C71 - 69
173(chain C and (resid 2 through 12 or resid 14...C170 - 221
183(chain C and (resid 2 through 12 or resid 14...C224 - 321
193(chain C and (resid 2 through 12 or resid 14...C323 - 338
213(chain F and (resid 2 through 12 or resid 14...F2 - 12
223(chain F and (resid 2 through 12 or resid 14...F14 - 32
233(chain F and (resid 2 through 12 or resid 14...F34 - 46
243(chain F and (resid 2 through 12 or resid 14...F48
253(chain F and (resid 2 through 12 or resid 14...F0
263(chain F and (resid 2 through 12 or resid 14...F55 - 56
273(chain F and (resid 2 through 12 or resid 14...F51 - 69
283(chain F and (resid 2 through 12 or resid 14...F71 - 125
293(chain F and (resid 2 through 12 or resid 14...F2 - 341
2103(chain F and (resid 2 through 12 or resid 14...F170 - 221
2113(chain F and (resid 2 through 12 or resid 14...F323 - 338

NCS ensembles :
ID
1
2
3

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Components

#1: Protein Predicted transcriptional regulator, XRE family


Mass: 11720.580 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli O127:H6 (strain E2348/69 / EPEC) (bacteria)
Strain: E2348/69 / EPEC / Gene: E2348C_3787 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: B7UL98
#2: Protein Couple_hipA domain-containing protein


Mass: 11713.165 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli O127:H6 (strain E2348/69 / EPEC) (bacteria)
Strain: E2348/69 / EPEC / Gene: E2348C_3786 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: B7UL97
#3: Protein HipA_C domain-containing protein


Mass: 39053.102 Da / Num. of mol.: 1 / Mutation: S59A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli O127:H6 (strain E2348/69 / EPEC) (bacteria)
Strain: E2348/69 / EPEC / Gene: E2348C_3785 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: B7UL96
#4: Protein HipA_C domain-containing protein


Mass: 39133.082 Da / Num. of mol.: 1 / Mutation: S59A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli O127:H6 (strain E2348/69 / EPEC) (bacteria)
Strain: E2348/69 / EPEC / Gene: E2348C_3785 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: B7UL96
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.58 Å3/Da / Density % sol: 65.71 % / Description: Plate
Crystal growTemperature: 293.15 K / Method: vapor diffusion, sitting drop / pH: 9 / Details: 0.1M BICINE, 6% MPD

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: MAX IV / Beamline: BioMAX / Wavelength: 1.5418 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: May 23, 2020
RadiationMonochromator: At 26 m, Si (111), double crystal monochromator, horizontally deflecting, LN2 side-cooling
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.5418 Å / Relative weight: 1
ReflectionResolution: 3.24→50.59 Å / Num. obs: 45019 / % possible obs: 81.9 % / Redundancy: 4.108 % / Biso Wilson estimate: 96.02 Å2 / CC1/2: 0.983 / Rmerge(I) obs: 0.332 / Rrim(I) all: 0.381 / Χ2: 0.83 / Net I/σ(I): 4.73 / Num. measured all: 184956 / Scaling rejects: 13
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
3.24-3.324.0973.3160.5512868400531410.1673.80778.4
3.32-3.424.1112.4210.7313791399933550.2042.7883.9
3.42-3.514.081.8940.9613097382632100.3092.17483.9
3.51-3.624.1521.611.313061379531460.4181.84482.9
3.62-3.744.0751.2861.6912063358829600.5061.47582.5
3.74-3.874.1131.1072.1812143354629520.5841.27283.2
3.87-4.024.1120.8232.8911412335927750.7120.94482.6
4.02-4.184.1040.7153.4111204332227300.8270.8282.2
4.18-4.374.0110.5144.210285311425640.8850.59182.3
4.37-4.584.1760.4145.6410139296224280.8990.47482
4.58-4.834.1990.3077.279965288523730.9450.35282.3
4.83-5.124.1540.2977.349085266421870.9430.3482.1
5.12-5.484.2270.2857.178708250420600.9460.32682.3
5.48-5.924.2370.2777.288178236119300.9490.31781.7
5.92-6.484.2030.2697.487478218917790.950.30781.3
6.48-7.253.7010.2017.855644196815250.960.23577.5
7.25-8.373.9240.11311.355391171613740.9870.1380.1
8.37-10.254.0790.0716.444773144711700.9940.07980.9
10.25-14.494.2840.05619.26387311359040.9950.06479.6
14.49-50.593.9430.04919.4817986074560.9960.05675.1

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Phasing

PhasingMethod: molecular replacement
Phasing MRModel details: Phaser MODE: MR_AUTO
Highest resolutionLowest resolution
Rotation7.4 Å50.59 Å
Translation7.4 Å50.59 Å

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Processing

Software
NameVersionClassification
PHENIX1.19rc5_4047refinement
XDSVERSION Mar 15, 2019 BUILT=20190315data reduction
XSCALEVERSION Mar 15, 2019 BUILT=20190315data scaling
PHASER2.8.3phasing
PDB_EXTRACT3.25data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PBD-code from depostion entry D_1292109701

Resolution: 3.34→50.59 Å / SU ML: 0.46 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 26.07 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2435 1085 4.82 %RANDOM
Rwork0.202 21448 --
obs0.2041 22533 87.59 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 295.56 Å2 / Biso mean: 103.8043 Å2 / Biso min: 39.31 Å2
Refinement stepCycle: final / Resolution: 3.34→50.59 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8233 0 0 8 8241
Biso mean---54.87 -
Num. residues----1023
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A410X-RAY DIFFRACTION8.674TORSIONAL
12D410X-RAY DIFFRACTION8.674TORSIONAL
21B490X-RAY DIFFRACTION8.674TORSIONAL
22E490X-RAY DIFFRACTION8.674TORSIONAL
31C1904X-RAY DIFFRACTION8.674TORSIONAL
32F1904X-RAY DIFFRACTION8.674TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 8

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
3.34-3.490.32531210.28552590271185
3.49-3.670.30981390.27272741288090
3.67-3.910.32241500.25442731288190
3.91-4.210.26251240.21852717284189
4.21-4.630.24141340.19552664279888
4.63-5.30.2181480.16982703285188
5.3-6.670.23631330.20662656278987
6.68-50.590.19541360.16142646278284
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.427-6.5631-4.77486.06074.43563.461-0.794-1.35290.73060.27230.7638-0.98060.76760.639-0.13170.88430.03560.20771.08370.13320.786394.98-27.03522.519
29.23726.22883.27974.39621.76444.34530.6913-1.1751-0.10021.9423-0.37470.03440.2738-0.52210.16550.66530.06630.07450.69670.00820.566690.2-15.42924.89
33.38431.64060.71046.3418-0.16775.637-0.011-0.03320.2361-0.6243-0.13070.6570.2124-0.28660.2330.5347-0.00920.16090.62260.03440.577486.743-12.73816.459
43.1947-1.49121.58397.56343.37063.24620.63212.0905-0.41710.08931.1251-0.60090.53011.0996-1.22491.03610.17610.18731.3727-0.41371.7561136.386-21.13918.158
55.3171.78612.2048.44673.11422.0494-1.00270.264-1.67830.57920.4383-0.19150.55621.1429-0.38991.3810.25290.22170.9603-0.28361.4297130.546-22.90319.898
64.6730.41660.48374.85872.58148.9271-0.4782-1.0107-1.7363-0.83470.3645-1.70612.16491.9120.19191.06230.32560.02650.90480.02631.6643130.226-26.28413.068
73.69811.03522.35345.87834.22885.99210.42590.2641-0.04751.192-0.73020.73390.6935-0.89730.44360.94240.16960.2591.1260.02331.4797120.235-20.9715.432
88.05182.95860.31062.00670.29752.09460.12060.9257-0.3376-0.6068-0.293-0.78471.97191.37170.32711.36980.24820.29341.2233-0.22421.696125.485-25.9738.826
97.25062.31234.16587.8957-1.42766.7186-0.79570.59090.1788-0.435-0.2828-1.60511.35330.22231.34180.76440.14810.01431.0785-0.27311.1936129.69-13.81610.586
106.04994.14263.48972.99682.12692.18150.6325-0.6752-0.7922-1.75350.68330.0126-0.5135-0.0701-1.64760.79440.17330.16441.6344-0.15651.8098137.7830.19519.175
116.03414.07433.10723.62391.18473.2312-0.85240.2006-0.05-1.70471.2412-1.39580.50681.82041.17171.8015-0.56690.72842.98320.05513.2228143.661-7.94413.239
127.95412.03914.07059.66992.67426.3862-0.22180.381-0.1795-1.19560.64540.6597-1.26560.25030.43311.29380.20340.29040.8955-0.00910.9013134.328-11.17621.723
134.99091.13731.20898.16514.80727.3687-0.5107-0.5173-1.58131.93930.1607-2.04291.54111.06760.40890.980.1271-0.17340.99930.3951.5957134.891-19.57722.825
146.1234-1.88471.67734.6469-1.65315.9638-0.1778-1.48960.53781.3105-0.0164-0.09460.1392-0.2234-0.11320.8178-0.07640.11141.0258-0.15210.6826113.16214.6540.574
155.9608-0.34082.30573.14070.87041.42980.0266-0.38030.4783-0.04910.05260.16110.15711.0746-0.19290.5649-0.1106-0.05920.8755-0.08390.517131.8579.00529.575
166.2653-0.73211.94235.7083-0.414.59510.2933-0.33370.117-0.1541-0.0188-0.63940.05440.3729-0.41270.60.08860.13450.907-0.04380.7287128.8774.39430.046
173.88360.90511.52972.1870.98592.42870.07430.0825-0.1144-0.0561-0.0751-0.20080.15020.2265-0.13190.67250.01120.20960.65290.04930.4556116.8461.36218.66
182.2464-0.12321.33111.82340.42393.20660.00370.42550.1451-0.3428-0.08630.0112-0.10910.09230.09580.85930.01770.26130.73780.01630.6383108.1926.9199.595
199.03850.51260.94844.71740.98513.63170.35610.1481.0433-0.6513-0.3514-0.0064-1.2046-0.52710.23560.8404-0.0010.21110.79540.13180.6676108.05218.66213.504
209.662-1.85497.88257.43291.28797.8087-0.15841.28311.0239-0.5537-0.9115-1.75310.74810.8433-0.61570.6835-0.10820.12710.9870.12990.72896.673-28.59910.337
218.38256.88342.88049.22364.78793.88960.35931.36110.0435-0.16450.12980.31370.13180.646-0.49850.7091-0.01920.03890.9811-0.05430.464886.509-32.2794.279
228.30462.37273.21627.60720.4491.30660.82090.60890.98130.7929-0.51971.5201-0.0984-0.2819-0.18390.7595-0.1090.18390.73310.03120.58372.712-35.6298.131
237.53924.22074.43234.47774.5284.56350.00010.20711.4743-0.6779-0.7491.5985-0.5667-0.06171.17451.1980.10840.03740.78990.0430.726978.317-24.5263.421
247.9864.1322.63645.14684.16364.8325-0.0321-0.68980.2674-0.52160.38050.9257-0.7929-0.723-0.40120.73640.03510.30310.5850.07960.515880.338-27.27613.858
257.58675.40964.14355.02614.31443.6087-0.90280.60950.19451.12721.2033-0.5977-0.14791.2849-0.39870.5501-0.0003-0.06530.5478-0.06570.538291.434-28.59516.902
260.39720.0298-1.82064.22-0.47628.16470.1816-2.3048-1.32760.8321-0.2398-0.90190.22831.8443-0.87981.08540.30880.05262.16710.42061.428107.726-62.0329.559
275.5558-3.332-0.8574.68680.84360.16890.3391-0.3531-0.75022.9529-0.8195-1.2015-1.43581.74461.35471.37910.4818-1.08842.3223-0.1731.2873104.507-60.13138.693
283.4885-0.7331-3.26883.4459-2.61896.7823-0.8633-0.81440.07810.32010.5346-1.85360.55672.28870.20630.7834-0.1738-0.18331.6538-0.38011.2247104.184-50.98530.8
293.3973-3.1626-3.1337.4621-2.53739.4855-0.2130.2977-0.48041.10170.17250.3947-0.43180.80790.29111.09240.1671-0.16011.5354-0.39411.204796.005-61.84933.192
304.76032.4223.03955.9641-3.9098.1837-0.68271.247-1.5507-2.5386-0.45032.71750.86550.1219-0.16741.80460.6998-0.1841.567-0.5711.438989.88-74.00523.681
313.8058-3.3133-2.7374.97930.65333.4149-1.369-0.3641-1.71330.1075-0.77320.73211.79820.13750.86832.01590.25520.1042.1902-0.06482.240494.652-79.01930.363
324.4022-2.86020.00045.013-0.27376.0953-0.0347-0.8468-0.13230.52660.53260.31430.50650.9032-1.0550.98110.1575-0.19931.33150.06611.2326103.077-67.95425.163
333.2987-0.9498-0.31014.8615-0.973.37390.37130.5887-0.2549-0.8999-0.288-0.57480.16390.83790.07361.03280.150.09580.7184-0.13990.70284.676-74.8142.156
344.6867-3.78671.18316.2831.82476.03930.18030.4302-0.1412-0.4185-0.0043-0.62430.75620.2685-0.17110.7169-0.04140.12120.6161-0.01010.557286.417-70.9738.476
351.824-1.7228-0.99084.41343.47585.1961-0.04850.1-0.0782-0.20970.06350.13120.41490.4364-0.26020.8720.1070.07770.8223-0.05860.77889.048-67.58614.631
361.992-1.56411.20742.73130.67784.4040.0078-0.19980.24930.2065-0.0853-0.24710.49190.02230.18960.5706-0.0630.08150.4910.01420.400978.963-54.40422.435
372.8761-0.7758-0.60673.84940.77085.0874-0.1095-0.0869-0.01150.51920.17160.42650.2656-0.2960.09030.5842-0.03940.11780.5263-0.03010.481169.856-56.09219.105
386.29770.8312-0.76874.713-1.27797.2559-0.7129-0.1547-0.30741.6631-0.14762.5820.7097-0.45160.60650.7782-0.07050.17120.7834-0.09581.141862.637-62.77612.272
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 36:44 )A36 - 44
2X-RAY DIFFRACTION2( CHAIN A AND RESID 45:59 )A45 - 59
3X-RAY DIFFRACTION3( CHAIN A AND RESID 60:107 )A60 - 107
4X-RAY DIFFRACTION4( CHAIN B AND RESID 2:9 )B2 - 9
5X-RAY DIFFRACTION5( CHAIN B AND RESID 10:19 )B10 - 19
6X-RAY DIFFRACTION6( CHAIN B AND RESID 20:33 )B20 - 33
7X-RAY DIFFRACTION7( CHAIN B AND RESID 34:43 )B34 - 43
8X-RAY DIFFRACTION8( CHAIN B AND RESID 44:50 )B44 - 50
9X-RAY DIFFRACTION9( CHAIN B AND RESID 51:60 )B51 - 60
10X-RAY DIFFRACTION10( CHAIN B AND RESID 61:73 )B61 - 73
11X-RAY DIFFRACTION11( CHAIN B AND RESID 74:81 )B74 - 81
12X-RAY DIFFRACTION12( CHAIN B AND RESID 82:95 )B82 - 95
13X-RAY DIFFRACTION13( CHAIN B AND RESID 96:101 )B96 - 101
14X-RAY DIFFRACTION14( CHAIN C AND RESID 2:28 )C2 - 28
15X-RAY DIFFRACTION15( CHAIN C AND RESID 29:57 )C29 - 57
16X-RAY DIFFRACTION16( CHAIN C AND RESID 58:94 )C58 - 94
17X-RAY DIFFRACTION17( CHAIN C AND RESID 95:219 )C95 - 219
18X-RAY DIFFRACTION18( CHAIN C AND RESID 220:313 )C220 - 313
19X-RAY DIFFRACTION19( CHAIN C AND RESID 314:340 )C314 - 340
20X-RAY DIFFRACTION20( CHAIN D AND RESID 36:44 )D36 - 44
21X-RAY DIFFRACTION21( CHAIN D AND RESID 45:59 )D45 - 59
22X-RAY DIFFRACTION22( CHAIN D AND RESID 60:73 )D60 - 73
23X-RAY DIFFRACTION23( CHAIN D AND RESID 74:86 )D74 - 86
24X-RAY DIFFRACTION24( CHAIN D AND RESID 87:98 )D87 - 98
25X-RAY DIFFRACTION25( CHAIN D AND RESID 99:107 )D99 - 107
26X-RAY DIFFRACTION26( CHAIN E AND RESID 2:19 )E2 - 19
27X-RAY DIFFRACTION27( CHAIN E AND RESID 20:28 )E20 - 28
28X-RAY DIFFRACTION28( CHAIN E AND RESID 29:50 )E29 - 50
29X-RAY DIFFRACTION29( CHAIN E AND RESID 51:60 )E51 - 60
30X-RAY DIFFRACTION30( CHAIN E AND RESID 61:65 )E61 - 65
31X-RAY DIFFRACTION31( CHAIN E AND RESID 66:80 )E66 - 80
32X-RAY DIFFRACTION32( CHAIN E AND RESID 81:101 )E81 - 101
33X-RAY DIFFRACTION33( CHAIN F AND RESID 2:58 )F2 - 58
34X-RAY DIFFRACTION34( CHAIN F AND RESID 59:120 )F59 - 120
35X-RAY DIFFRACTION35( CHAIN F AND RESID 121:165 )F121 - 165
36X-RAY DIFFRACTION36( CHAIN F AND RESID 166:220 )F166 - 220
37X-RAY DIFFRACTION37( CHAIN F AND RESID 221:313 )F221 - 313
38X-RAY DIFFRACTION38( CHAIN F AND RESID 314:341 )F314 - 341

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