+Open data
-Basic information
Entry | Database: PDB / ID: 7a67 | ||||||
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Title | Pcc1Pcc2 complex | ||||||
Components |
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Keywords | STRUCTURAL PROTEIN / t6A synthases | ||||||
Function / homology | CTAG/Pcc1 family / Transcription factor Pcc1 / EKC/KEOPS complex / tRNA threonylcarbamoyladenosine modification / cytoplasm / KEOPS complex subunit Pcc1 Function and homology information | ||||||
Biological species | Pyrococcus abyssi (archaea) Pyrococcus abyssi GE5 (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.18 Å | ||||||
Authors | Missoury, S. / Tilbeurgh, V.H. | ||||||
Citation | Journal: To Be Published Title: Pcc1Pcc2 structure Authors: Missoury, S. / Tilbeurgh, V.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7a67.cif.gz | 46.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7a67.ent.gz | 31.7 KB | Display | PDB format |
PDBx/mmJSON format | 7a67.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7a67_validation.pdf.gz | 432.9 KB | Display | wwPDB validaton report |
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Full document | 7a67_full_validation.pdf.gz | 435.4 KB | Display | |
Data in XML | 7a67_validation.xml.gz | 8.3 KB | Display | |
Data in CIF | 7a67_validation.cif.gz | 10.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a6/7a67 ftp://data.pdbj.org/pub/pdb/validation_reports/a6/7a67 | HTTPS FTP |
-Related structure data
Related structure data | 3encS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 10408.970 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyrococcus abyssi (strain GE5 / Orsay) (archaea) Strain: GE5 / Orsay / Gene: pcc1, PYRAB02750, PAB3073 Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) References: UniProt: Q9V1Z9 |
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#2: Protein | Mass: 10342.880 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyrococcus abyssi GE5 (archaea) Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.15 Å3/Da / Density % sol: 60.98 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / Details: 15% PEG 6000 and 5% glycerol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 2 / Wavelength: 0.980066 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS EIGER2 X 9M / Detector: PIXEL / Date: Sep 14, 2018 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.980066 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 3.17→34.95 Å / Num. obs: 4659 / % possible obs: 98.4 % / Redundancy: 18.751 % / Biso Wilson estimate: 90.58 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.212 / Rrim(I) all: 0.218 / Χ2: 1.105 / Net I/σ(I): 10.93 / Num. measured all: 87363 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3ENC Resolution: 3.18→34.95 Å / Cor.coef. Fo:Fc: 0.919 / Cor.coef. Fo:Fc free: 0.894 / Cross valid method: THROUGHOUT / σ(F): 0 / SU Rfree Blow DPI: 0.442
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Displacement parameters | Biso max: 136.84 Å2 / Biso mean: 60.74 Å2 / Biso min: 15.15 Å2
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Refine analyze | Luzzati coordinate error obs: 0.39 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.18→34.95 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.18→3.27 Å / Rfactor Rfree error: 0 / Total num. of bins used: 21
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