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Yorodumi- PDB-6zxp: Solution structure of the C-terminal domain of the vaccinia virus... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6zxp | ||||||
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Title | Solution structure of the C-terminal domain of the vaccinia virus DNA polymerase processivity factor component A20 fused to a short peptide from the viral DNA polymerase E9. | ||||||
Components | DNA polymerase processivity factor component A20,DNA polymerase processivity factor component E9 | ||||||
Keywords | REPLICATION / Poxviridae / DNA polymerase holoenzyme / processivity factor binding | ||||||
Function / homology | Chordopoxvirus A20R / Chordopoxvirus A20R protein / viral DNA genome replication / DNA replication / DNA polymerase processivity factor component OPG148 Function and homology information | ||||||
Biological species | Vaccinia virus Copenhagen | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Authors | Bersch, B. / Tarbouriech, N. / Burmeister, W. / Iseni, F. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2021 Title: Solution Structure of the C-terminal Domain of A20, the Missing Brick for the Characterization of the Interface between Vaccinia Virus DNA Polymerase and its Processivity Factor. Authors: Bersch, B. / Tarbouriech, N. / Burmeister, W.P. / Iseni, F. #1: Journal: Nature Communications / Year: 2017 Title: The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding. Authors: Tarbouriech, N. / Ducournau, C. / Hutin, S. / Mas, P.J. / Man, P. / Forest, E. / Hart, D.J. / Peyrefitte, C.N. / Burmeister, W. / Iseni, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6zxp.cif.gz | 1002.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6zxp.ent.gz | 855.5 KB | Display | PDB format |
PDBx/mmJSON format | 6zxp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6zxp_validation.pdf.gz | 551.3 KB | Display | wwPDB validaton report |
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Full document | 6zxp_full_validation.pdf.gz | 1.7 MB | Display | |
Data in XML | 6zxp_validation.xml.gz | 271.8 KB | Display | |
Data in CIF | 6zxp_validation.cif.gz | 195.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zx/6zxp ftp://data.pdbj.org/pub/pdb/validation_reports/zx/6zxp | HTTPS FTP |
-Related structure data
Related structure data | 6zycC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16882.934 Da / Num. of mol.: 1 / Mutation: L147A Source method: isolated from a genetically manipulated source Details: This is a recombinant fusion protein: residues 2-124 correspond to A20_VACCC:304-426, residues 125-135 to a flexible linker and residues 136-150 to E9_VACCC:576-590. E9_VACCC:L587A ...Details: This is a recombinant fusion protein: residues 2-124 correspond to A20_VACCC:304-426, residues 125-135 to a flexible linker and residues 136-150 to E9_VACCC:576-590. E9_VACCC:L587A mutation,This is a recombinant fusion protein: residues 2-124 correspond to A20_VACCC:304-426, residues 125-135 to a flexible linker and residues 136-150 to E9_VACCC:576-590. E9_VACCC:L587A mutation Source: (gene. exp.) Vaccinia virus Copenhagen / Gene: A20R / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): -Star / References: UniProt: P20995 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Details: 3 mm tube / Ionic strength: 0.3 M / Label: general / pH: 6 / PH err: 0.1 / Pressure: 1 atm / Temperature: 298 K / Temperature err: 0.5 |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 6 / Details: in explicit water | |||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 1000 / Conformers submitted total number: 20 |