Cell/Rod shape-determining protein MreC, domain 1 / Rod shape-determining protein MreC / Cell/Rod shape-determining protein MreC, domain 2 / rod shape-determining protein MreC / regulation of cell shape / plasma membrane / Cell shape-determining protein MreC
ジャーナル: Nat Commun / 年: 2021 タイトル: Self-association of MreC as a regulatory signal in bacterial cell wall elongation. 著者: Alexandre Martins / Carlos Contreras-Martel / Manon Janet-Maitre / Mayara M Miyachiro / Leandro F Estrozi / Daniel Maragno Trindade / Caíque C Malospirito / Fernanda Rodrigues-Costa / Lionel ...著者: Alexandre Martins / Carlos Contreras-Martel / Manon Janet-Maitre / Mayara M Miyachiro / Leandro F Estrozi / Daniel Maragno Trindade / Caíque C Malospirito / Fernanda Rodrigues-Costa / Lionel Imbert / Viviana Job / Guy Schoehn / Ina Attrée / Andréa Dessen / 要旨: The elongasome, or Rod system, is a protein complex that controls cell wall formation in rod-shaped bacteria. MreC is a membrane-associated elongasome component that co-localizes with the ...The elongasome, or Rod system, is a protein complex that controls cell wall formation in rod-shaped bacteria. MreC is a membrane-associated elongasome component that co-localizes with the cytoskeletal element MreB and regulates the activity of cell wall biosynthesis enzymes, in a process that may be dependent on MreC self-association. Here, we use electron cryo-microscopy and X-ray crystallography to determine the structure of a self-associated form of MreC from Pseudomonas aeruginosa in atomic detail. MreC monomers interact in head-to-tail fashion. Longitudinal and lateral interfaces are essential for oligomerization in vitro, and a phylogenetic analysis of proteobacterial MreC sequences indicates the prevalence of the identified interfaces. Our results are consistent with a model where MreC's ability to alternate between self-association and interaction with the cell wall biosynthesis machinery plays a key role in the regulation of elongasome activity.
構造決定の手法: AB INITIO PHASING / 解像度: 1.471→42.435 Å / Cor.coef. Fo:Fc: 0.966 / Cor.coef. Fo:Fc free: 0.955 / SU B: 4.582 / SU ML: 0.082 / 交差検証法: FREE R-VALUE / ESU R: 0.082 / ESU R Free: 0.083 / 詳細: Hydrogens have been added in their riding positions
Rfactor
反射数
%反射
Rfree
0.2445
2036
7.473 %
Rwork
0.2168
25210
-
all
0.219
-
-
obs
-
27246
96.262 %
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: BABINET MODEL PLUS MASK
原子変位パラメータ
Biso mean: 39.496 Å2
Baniso -1
Baniso -2
Baniso -3
1-
0.658 Å2
0.329 Å2
-0 Å2
2-
-
0.658 Å2
0 Å2
3-
-
-
-2.133 Å2
精密化ステップ
サイクル: LAST / 解像度: 1.471→42.435 Å
タンパク質
核酸
リガンド
溶媒
全体
原子数
1178
0
6
66
1250
拘束条件
Refine-ID
タイプ
Dev ideal
Dev ideal target
数
X-RAY DIFFRACTION
r_bond_refined_d
0.01
0.013
1198
X-RAY DIFFRACTION
r_bond_other_d
0.006
0.017
1162
X-RAY DIFFRACTION
r_angle_refined_deg
1.332
1.643
1631
X-RAY DIFFRACTION
r_angle_other_deg
1.292
1.573
2683
X-RAY DIFFRACTION
r_dihedral_angle_1_deg
6.701
5
154
X-RAY DIFFRACTION
r_dihedral_angle_2_deg
30.244
21.5
60
X-RAY DIFFRACTION
r_dihedral_angle_3_deg
12.028
15
198
X-RAY DIFFRACTION
r_dihedral_angle_4_deg
19.598
15
10
X-RAY DIFFRACTION
r_chiral_restr
0.067
0.2
157
X-RAY DIFFRACTION
r_gen_planes_refined
0.01
0.02
1343
X-RAY DIFFRACTION
r_gen_planes_other
0.001
0.02
239
X-RAY DIFFRACTION
r_nbd_refined
0.214
0.2
179
X-RAY DIFFRACTION
r_symmetry_nbd_other
0.198
0.2
1087
X-RAY DIFFRACTION
r_nbtor_refined
0.155
0.2
580
X-RAY DIFFRACTION
r_symmetry_nbtor_other
0.079
0.2
553
X-RAY DIFFRACTION
r_xyhbond_nbd_refined
0.143
0.2
42
X-RAY DIFFRACTION
r_symmetry_xyhbond_nbd_other
0.148
0.2
1
X-RAY DIFFRACTION
r_symmetry_nbd_refined
0.22
0.2
11
X-RAY DIFFRACTION
r_nbd_other
0.236
0.2
46
X-RAY DIFFRACTION
r_symmetry_xyhbond_nbd_refined
0.134
0.2
6
X-RAY DIFFRACTION
r_mcbond_it
1.351
1.724
619
X-RAY DIFFRACTION
r_mcbond_other
1.349
1.723
618
X-RAY DIFFRACTION
r_mcangle_it
1.766
2.582
772
X-RAY DIFFRACTION
r_mcangle_other
1.765
2.583
773
X-RAY DIFFRACTION
r_scbond_it
1.885
1.969
579
X-RAY DIFFRACTION
r_scbond_other
1.883
1.97
580
X-RAY DIFFRACTION
r_scangle_it
2.838
2.862
859
X-RAY DIFFRACTION
r_scangle_other
2.837
2.862
860
X-RAY DIFFRACTION
r_lrange_it
5.885
21.132
1188
X-RAY DIFFRACTION
r_lrange_other
5.87
20.916
1184
LS精密化 シェル
Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20