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- PDB-6zj2: Structure of RcsB from Salmonella enterica serovar Typhimurium bo... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6zj2 | |||||||||||||||
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Title | Structure of RcsB from Salmonella enterica serovar Typhimurium bound to promoter rprA in the presence of phosphomimetic BeF3- | |||||||||||||||
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![]() | DNA BINDING PROTEIN / response regulator / phosphorylation / two-component systems / transcriptional factor | |||||||||||||||
Function / homology | ![]() phosphorelay signal transduction system / regulation of DNA-templated transcription / DNA binding Similarity search - Function | |||||||||||||||
Biological species | ![]() ![]() | |||||||||||||||
Method | ![]() ![]() ![]() | |||||||||||||||
![]() | Huesa, J. / Marina, A. / Casino, P. | |||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure-based analyses of Salmonella RcsB variants unravel new features of the Rcs regulon. Authors: Huesa, J. / Giner-Lamia, J. / Pucciarelli, M.G. / Paredes-Martinez, F. / Portillo, F.G. / Marina, A. / Casino, P. | |||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 320.1 KB | Display | ![]() |
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PDB format | ![]() | 247.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 513.7 KB | Display | ![]() |
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Full document | ![]() | 533.7 KB | Display | |
Data in XML | ![]() | 49.8 KB | Display | |
Data in CIF | ![]() | 68.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ziiC ![]() 6zilC ![]() 6zixC ![]() 5o8zS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23743.650 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: rcsB, STM2270 / Production host: ![]() ![]() #2: DNA chain | Mass: 7080.584 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() #3: DNA chain | Mass: 7040.561 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() #4: Chemical | #5: Chemical | ChemComp-MG / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.59 Å3/Da / Density % sol: 52.55 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / Details: 15% PEG 8000, MES pH 6.5 and 0.2M NaAc |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Sep 26, 2019 | |||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97949 Å / Relative weight: 1 | |||||||||||||||||||||||||||
Reflection | Resolution: 3.38→45.99 Å / Num. obs: 30572 / % possible obs: 99.1 % / Redundancy: 3.8 % / CC1/2: 0.983 / Rmerge(I) obs: 0.228 / Rpim(I) all: 0.133 / Rrim(I) all: 0.266 / Net I/σ(I): 4.2 | |||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1 / Redundancy: 3.6 %
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5O8Z Resolution: 3.38→45.99 Å / Cor.coef. Fo:Fc: 0.866 / Cor.coef. Fo:Fc free: 0.829 / SU B: 22.271 / SU ML: 0.389 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.151 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 195.64 Å2 / Biso mean: 85.244 Å2 / Biso min: 35.82 Å2
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Refinement step | Cycle: final / Resolution: 3.38→45.99 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.38→3.468 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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