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- PDB-5vxn: Structure of two RcsB dimers bound to two parallel DNAs. -

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Basic information

Entry
Database: PDB / ID: 5vxn
TitleStructure of two RcsB dimers bound to two parallel DNAs.
Components
  • DNA (5'-D(*GP*AP*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*AP*TP*A)-3')
  • DNA (5'-D(*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*A)-3')
  • Transcriptional regulatory protein RcsB
Keywordsdna binding protein/dna / Rcs phosphorelay / response regulator / FixJ/NarL / Center for Structural Genomics of Infectious Diseases / CSGID / DNA-binding protein / dna binding protein-dna complex
Function / homology
Function and homology information


regulation of capsule organization / regulation of carbohydrate catabolic process / single-species biofilm formation on inanimate substrate / positive regulation of cell projection organization / cellular stress response to acidic pH / regulation of bacterial-type flagellum-dependent cell motility / DNA-binding transcription activator activity / DNA-binding transcription repressor activity / phosphorelay signal transduction system / transcription regulator complex ...regulation of capsule organization / regulation of carbohydrate catabolic process / single-species biofilm formation on inanimate substrate / positive regulation of cell projection organization / cellular stress response to acidic pH / regulation of bacterial-type flagellum-dependent cell motility / DNA-binding transcription activator activity / DNA-binding transcription repressor activity / phosphorelay signal transduction system / transcription regulator complex / response to antibiotic / negative regulation of DNA-templated transcription / DNA-templated transcription / regulation of DNA-templated transcription / positive regulation of DNA-templated transcription / DNA binding / identical protein binding / cytosol
Similarity search - Function
Transcriptional regulatory protein RcsB / : / LuxR-type HTH domain signature. / LuxR-type HTH domain profile. / Transcription regulator LuxR, C-terminal / Bacterial regulatory proteins, luxR family / helix_turn_helix, Lux Regulon / Signal transduction response regulator, C-terminal effector / Response regulator receiver domain / cheY-homologous receiver domain ...Transcriptional regulatory protein RcsB / : / LuxR-type HTH domain signature. / LuxR-type HTH domain profile. / Transcription regulator LuxR, C-terminal / Bacterial regulatory proteins, luxR family / helix_turn_helix, Lux Regulon / Signal transduction response regulator, C-terminal effector / Response regulator receiver domain / cheY-homologous receiver domain / Signal transduction response regulator, receiver domain / Response regulatory domain profile. / CheY-like superfamily / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
DNA / DNA (> 10) / Transcriptional regulatory protein RcsB
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3.375 Å
AuthorsFilippova, E.V. / Minasov, G. / Pshenychnyi, S. / Anderson, W.F. / Center for Structural Genomics of Infectious Diseases (CSGID)
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) United States
CitationJournal: MBio / Year: 2018
Title: Structural Basis for DNA Recognition by the Two-Component Response Regulator RcsB.
Authors: Filippova, E.V. / Zemaitaitis, B. / Aung, T. / Wolfe, A.J. / Anderson, W.F.
History
DepositionMay 23, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 7, 2018Provider: repository / Type: Initial release
Revision 1.1Mar 14, 2018Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.name
Revision 1.2Dec 11, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.3Mar 13, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Transcriptional regulatory protein RcsB
B: Transcriptional regulatory protein RcsB
E: DNA (5'-D(*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*A)-3')
F: DNA (5'-D(*GP*AP*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*AP*TP*A)-3')
C: Transcriptional regulatory protein RcsB
D: Transcriptional regulatory protein RcsB
G: DNA (5'-D(*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*A)-3')
H: DNA (5'-D(*GP*AP*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*AP*TP*A)-3')


Theoretical massNumber of molelcules
Total (without water)116,8538
Polymers116,8538
Non-polymers00
Water21612
1
A: Transcriptional regulatory protein RcsB
B: Transcriptional regulatory protein RcsB
E: DNA (5'-D(*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*A)-3')
F: DNA (5'-D(*GP*AP*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*AP*TP*A)-3')


Theoretical massNumber of molelcules
Total (without water)58,4264
Polymers58,4264
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6300 Å2
ΔGint-59 kcal/mol
Surface area23490 Å2
MethodPISA
2
C: Transcriptional regulatory protein RcsB
D: Transcriptional regulatory protein RcsB
G: DNA (5'-D(*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*A)-3')
H: DNA (5'-D(*GP*AP*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*AP*TP*A)-3')


Theoretical massNumber of molelcules
Total (without water)58,4264
Polymers58,4264
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6360 Å2
ΔGint-61 kcal/mol
Surface area23710 Å2
MethodPISA
Unit cell
Length a, b, c (Å)74.116, 113.231, 76.868
Angle α, β, γ (deg.)90.00, 115.37, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Protein
Transcriptional regulatory protein RcsB / Capsular synthesis regulator component B


Mass: 23699.600 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (strain K12) (bacteria)
Strain: K12 / Gene: rcsB, b2217, JW2205 / Plasmid: pCA24n / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 DE3 magic / References: UniProt: P0DMC7
#2: DNA chain DNA (5'-D(*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*A)-3')


Mass: 5562.653 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Escherichia coli (E. coli)
#3: DNA chain DNA (5'-D(*GP*AP*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*AP*TP*A)-3')


Mass: 5464.577 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Escherichia coli (E. coli)
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.49 Å3/Da / Density % sol: 50.68 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 0.2 M Sodium Chloride, 0.1 M HEPES, 25% PEG3350

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-G / Wavelength: 0.97857 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jun 16, 2016 / Details: beryllium lenses
RadiationMonochromator: C(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97857 Å / Relative weight: 1
ReflectionResolution: 3.37→113.23 Å / Num. obs: 15089 / % possible obs: 93 % / Redundancy: 4.3 % / Biso Wilson estimate: 103 Å2 / CC1/2: 0.99 / Rmerge(I) obs: 0.1 / Net I/σ(I): 10.8
Reflection shellResolution: 3.37→3.39 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.6 / Mean I/σ(I) obs: 2.1 / Num. unique all: 163 / % possible all: 100

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Processing

Software
NameVersionClassification
PHENIX(1.10_2155: ???)refinement
XDSdata processing
XDSdata scaling
PHASERphasing
BLU-MAXdata collection
Cootmodel building
RefinementResolution: 3.375→56.615 Å / SU ML: 0.5 / σ(F): 1.36 / Phase error: 29.84 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2683 794 5.28 %
Rwork0.1985 --
obs0.202 15041 92.76 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3.375→56.615 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6141 1464 0 12 7617
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0037854
X-RAY DIFFRACTIONf_angle_d0.55610912
X-RAY DIFFRACTIONf_dihedral_angle_d18.4864584
X-RAY DIFFRACTIONf_chiral_restr0.0411314
X-RAY DIFFRACTIONf_plane_restr0.0031118
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.3755-3.58690.3267940.28341874X-RAY DIFFRACTION74
3.5869-3.86380.32191320.26732559X-RAY DIFFRACTION99
3.8638-4.25250.31751090.22852270X-RAY DIFFRACTION88
4.2525-4.86760.27921160.19712548X-RAY DIFFRACTION99
4.8676-6.13140.27261970.20072486X-RAY DIFFRACTION99
6.1314-56.62340.22011460.15592510X-RAY DIFFRACTION96
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.4249-0.3804-0.71740.9705-0.12921.4549-1.6904-0.29330.0045-1.08880.0171-0.0285-0.17060.2304-0.05120.51070.0393-0.08341.177-0.28850.922721.6213-33.146586.4114
26.5274-3.3668-0.20784.0963-0.02550.00060.4770.55851.193-0.37280.55550.53011.9831-2.32620.32960.9853-0.0485-0.06141.390.21150.685523.1362-25.709780.67
31.144-0.7527-1.43322.5441.60373.0516-0.2976-0.0684-0.22780.0167-0.44310.5994-0.0993-0.5751-1.34390.4963-0.0610.04061.14480.06290.494723.2006-32.165392.2953
43.1308-0.308-2.25674.843-0.2273.04940.0123-1.7622-2.8021-0.2996-1.28541.06710.55231.2234-1.73450.5698-0.29810.58390.92531.1081-0.244528.6266-40.23395.1987
51.4609-0.4446-0.33890.40820.68620.61440.5432-0.0091-0.57310.154-0.65711.14071.11410.0806-0.00010.9224-0.04770.15961.0208-0.05450.903333.6249-39.554182.1954
60.24880.46060.03330.7137-0.04230.00470.2532-0.3236-0.9261.03391.4548-2.25792.6193-0.18350.13030.9556-0.2007-0.5211.5043-0.39812.706626.1303-47.449979.123
70.4547-0.07250.13460.9827-0.89260.75510.91682.4619-1.3505-3.30550.63881.97312.68920.67450.06591.41-0.20620.17351.0911-0.20721.449642.8889-44.036677.3289
81.44671.1830.06770.8022-0.00370.32930.9595-2.47820.1866-2.0533-0.09890.48731.4161-1.05260.0082-1.80032.5775-1.8455-0.41441.6969-0.507331.6882-14.717969.7349
92.53780.1515-1.72243.5779-1.15834.4586-0.18120.7774-2.7291-1.3048-0.12350.43480.7329-1.1689-1.29630.1171-0.1789-0.17061.11720.07021.210227.6102-21.756974.1546
101.21580.24070.70191.2798-0.30170.5488-0.50210.98880.91170.22560.32210.2619-0.0787-0.9081-00.79720.1771-0.00890.93910.13880.673833.4871-14.813766.1849
111.39620.06540.51620.0627-0.1560.9103-0.07120.73370.5175-0.3480.37610.7203-0.3028-0.0320.00060.84060.10850.06750.93130.160.765547.7165-14.585271.203
120.69450.52710.39720.32740.17690.09851.11380.3271-0.42180.1032-1.5251-0.6103-0.19990.9938-0.03770.79930.20480.3480.88650.14051.116539.0598-8.8372.0288
130.7280.26910.00061.8331-2.14072.9346-1.0523-1.1438-0.7191.72122.22690.5819-2.40111.1660.05251.5433-0.07750.65961.2617-0.40261.988744.0329-12.107186.2788
145.36094.7339-3.75667.3419-8.53662.01790.2061-0.30892.5472-1.84250.2270.29680.8338-0.94140.26811.16540.01770.35850.7152-0.11530.778738.2512-7.158479.9567
153.25772.61225.31612.46394.20568.92232.4485-0.58210.93830.1145-0.77410.57111.2585-0.47971.19340.85630.64270.33361.2171-0.0921.585128.0374-9.764580.6802
160.91120.28940.3050.24490.09980.0914-2.8365-0.693-0.4749-0.34630.31560.37290.0668-0.7083-0.09091.43330.45150.10521.629-0.62231.838933.139-4.92489.1043
170.1474-0.11380.23850.0479-0.17910.3911-1.46130.58311.4487-1.31281.1824-0.0683-0.56020.10970.00051.6467-0.0272-0.21781.05920.22441.468169.0165-50.083292.3429
180.247-0.0491-1.070.20830.86942.31770.4864-0.6715-0.32760.1337-0.4438-3.3557-0.6030.28370.00971.0726-0.0948-0.16011.0083-0.16611.643371.9477-28.150386.3891
190.0333-0.0350.06730.0304-0.0111-0.00910.3093-0.18863.95560.4073-1.3957-0.2743-0.6302-0.7794-0.00041.4185-0.0208-0.12531.0984-0.08872.125872.5467-5.385983.6818
200.0884-0.25620.21650.5334-0.5650.35310.74490.6283-0.13831.1338-1.2605-0.0667-0.1587-0.81530.00271.2305-0.2720.12430.8406-0.09491.507766.4654-11.223482.1079
21-0.115-0.26570.08491.7817-1.19522.11671.0162-0.2727-0.48060.7754-0.9228-2.6416-0.53510.694-0.02530.9530.0213-0.00361.0221-0.08211.405172.0924-32.688686.7328
220.031-0.05520.1360.0331-0.09760.07471.4761-1.1499-0.9056-0.7199-0.93460.1096-0.5690.7861-0.00331.46790.0010.07211.14040.02641.482873.2633-55.543887.3815
230.6182-0.4846-1.0140.94620.07331.78680.01490.4343-0.51020.2814-0.31940.10040.46150.4930.00060.9362-0.17770.1440.8002-0.04511.105911.5406-30.2952120.6757
241.5876-1.9499-0.88872.4291-0.05294.2313-0.04760.0431-0.42710.7588-0.16540.84830.4798-0.2702-0.00091.0063-0.17670.14230.6948-0.07740.713815.0095-36.4383129.7908
250.36240.4328-0.21090.78680.33830.3773-0.29010.63170.8194-0.88710.64072.5193-0.24040.49370.00250.8306-0.15690.10440.88320.05480.803930.5312-40.2737122.6961
26-0.140.13050.14130.10890.27540.3484-0.20420.7954-0.25130.5784-0.04570.03170.82480.3648-0.01680.6948-0.1409-0.11090.8441-0.08030.589118.3954-41.6269115.1885
275.49842.904-3.93657.0806-6.52676.41271.25351.68480.2085-2.1651.1941-1.35011.81071.59731.90881.23110.05840.05020.9236-0.38911.033233.3307-44.0935113.3265
280.28080.1674-0.49530.22290.34221.6261-0.01470.6835-0.1269-0.5342-0.15490.4750.1609-0.9994-0.51480.63170.0217-0.08791.00450.43310.807818.3192-18.9846110.4563
290.40920.4522-0.64851.79730.12312.53770.72331.63341.2184-0.5499-0.89711.1555-0.8171-1.0925-0.22150.94090.4582-0.3750.86080.5353-0.126720.4982-15.8093105.3135
302.37711.2191-0.79690.25780.12371.44950.2710.795-0.0704-0.56740.0224-0.121-0.54720.003-00.7981-0.0940.02790.77160.1210.719532.0136-13.328107.4417
311.5761-0.5396-0.8599-0.1722-0.00120.65350.0606-0.06731.0820.30190.70330.6739-0.93570.320.04821.01820.17160.11550.4610.02730.997431.1647-11.2775118.7071
320.35120.1307-0.22910.2373-0.72831.93020.17110.11560.8646-0.123-0.09240.573-0.0069-1.0707-0.00620.83910.10650.08590.59250.04970.88920.7267-10.5454120.2198
332.14371.18540.99690.64880.50840.4278-0.24261.2671-0.16960.43291.5032.0625-0.45930.78160.0141.37750.24340.00460.92820.01851.317224.2909-2.2724123.5423
343.83693.6975-4.09323.7965-3.39875.737-0.0583-1.1473-0.31151.2931-0.5651.413-1.55190.79520.05621.16950.2215-0.3660.9460.1021.25259.6766-49.7694125.869
350.86950.0252-0.33870.0684-0.33010.1024-0.83570.72611.2583-0.4327-0.3534-2.46190.22570.0979-0.00490.99320.11550.1240.88520.05811.559962.8748-36.3538115.3363
360.1373-0.20690.49840.6786-0.87070.80520.3024-0.9285-0.0744-1.3019-0.6931-1.606-1.0438-0.22690.00451.1305-0.0924-0.23490.8977-0.11781.380860.7922-19.6329122.9426
370.3882-0.2666-0.98830.23220.50923.34891.4321-0.32070.2763.3173-3.2945-1.6318-1.15780.2617-0.10921.8331-0.0744-0.38471.0895-0.17852.383762.1203-5.4131116.2296
380.0420.2704-0.14990.6-0.274-0.00031.099-1.1372-0.52870.48840.3444-1.60110.55510.68770.0141.3581-0.1688-0.08840.9044-0.05241.710255.6673-11.3115.6728
390.3765-0.1301-0.40910.06720.20640.14780.14220.670.59170.5302-0.489-2.5686-0.5830.78810.01631.157-0.1229-0.19831.17240.0092.01765.9827-24.6965120.842
400.5766-1.03561.24481.3671-1.73631.9621-0.383-0.0377-1.23370.12820.627-1.20460.19440.44790.16510.8377-0.0715-0.14370.7246-0.20461.292857.8155-41.0208117.6117
41-0.02590.0380.03840.56720.37160.2217-0.17450.6518-2.4092-0.97590.0856-0.4823-0.9321-0.105-0.00451.08140.23150.02141.23410.12011.374363.132-55.464120.428
423.5637-3.2425-0.09822.3872-0.160.6343-0.1694-0.10360.60840.46410.4522-1.0339-0.09880.08640.01250.5465-0.1857-0.05870.4878-0.0960.845646.4936-18.2352120.1058
432.31471.6151.52298.4356-4.83125.80060.0012-1.10440.13261.50561.05430.72251.41861.07263.04531.0454-0.00620.02690.7119-0.15230.631543.1854-23.4488132.2163
444.1319-0.3844-3.48082.67462.62635.5634-1.6167-0.584-1.28421.844-0.137-0.53581.9497-1.9632-1.08580.84850.3568-0.3260.94940.14840.547355.1094-44.822397.0499
450.30551.12810.05923.64860.0181-0.01790.4058-1.77430.5027-0.31590.0348-1.8522-0.8572-0.0399-0.06971.33610.09-0.24960.8622-0.04640.867865.6855-38.7822100.8414
460.9333-0.40451.7951.3163-0.00192.6962-0.1763-1.0207-1.2922-0.02120.30470.30890.058-0.6867-0.00030.82030.15360.04250.94420.04250.954955.2933-39.80790.1885
477.49793.4791-5.21362.4427-1.51874.71110.5514-0.36531.82520.59312.02230.5656-2.12451.07165.08010.29880.403-0.62160.4733-0.07230.482252.5053-15.508585.3228
480.3776-0.27450.04320.72670.55430.5396-0.2097-0.04271.8807-0.61561.07630.1724-0.21820.06310.00710.50120.0483-0.05630.772-0.00040.881758.0185-22.939678.0614
496.8434-1.40380.24871.8414-1.11510.8097-1.105-3.39330.76790.3096-0.2928-0.4826-1.75920.2105-5.70561.0163-0.1363-0.23050.579-0.57221.053556.3659-19.960692.2374
508.7034.71043.93935.29162.23621.7175-0.283-3.13490.62680.8677-0.02520.8672-0.0920.7786-0.59210.74750.21970.08281.24440.5420.199646.398-44.5271132.4015
510.14440.1938-0.15490.3032-0.05970.1476-0.3443-2.8497-0.01821.25420.7145-0.62720.35361.3773-0.00141.1050.2572-0.221.1295-0.11650.986357.3807-38.3349134.4699
527.74281.93080.45131.56320.2853-0.00210.30931.2153-1.4643-0.7703-0.5743-0.7901-0.0161-0.392-0.04780.83280.2268-0.08540.81710.14530.368251.0834-43.4876124.4822
530.2819-0.10640.52080.8228-1.4473.10360.7327-0.57080.35310.17120.74831.3758-1.557-2.10.08080.91330.00920.06790.736-0.01370.63839.8531-36.7675126.3919
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 2 through 12 )
2X-RAY DIFFRACTION2chain 'A' and (resid 13 through 24 )
3X-RAY DIFFRACTION3chain 'A' and (resid 25 through 66 )
4X-RAY DIFFRACTION4chain 'A' and (resid 67 through 87 )
5X-RAY DIFFRACTION5chain 'A' and (resid 88 through 123 )
6X-RAY DIFFRACTION6chain 'A' and (resid 124 through 131 )
7X-RAY DIFFRACTION7chain 'A' and (resid 132 through 141 )
8X-RAY DIFFRACTION8chain 'B' and (resid 2 through 12 )
9X-RAY DIFFRACTION9chain 'B' and (resid 13 through 23 )
10X-RAY DIFFRACTION10chain 'B' and (resid 24 through 55 )
11X-RAY DIFFRACTION11chain 'B' and (resid 56 through 77 )
12X-RAY DIFFRACTION12chain 'B' and (resid 78 through 87 )
13X-RAY DIFFRACTION13chain 'B' and (resid 88 through 101 )
14X-RAY DIFFRACTION14chain 'B' and (resid 102 through 108 )
15X-RAY DIFFRACTION15chain 'B' and (resid 109 through 124 )
16X-RAY DIFFRACTION16chain 'B' and (resid 125 through 139 )
17X-RAY DIFFRACTION17chain 'E' and (resid 1 through 5 )
18X-RAY DIFFRACTION18chain 'E' and (resid 6 through 15 )
19X-RAY DIFFRACTION19chain 'E' and (resid 16 through 18 )
20X-RAY DIFFRACTION20chain 'F' and (resid 20 through 24 )
21X-RAY DIFFRACTION21chain 'F' and (resid 25 through 34 )
22X-RAY DIFFRACTION22chain 'F' and (resid 35 through 37 )
23X-RAY DIFFRACTION23chain 'C' and (resid 2 through 24 )
24X-RAY DIFFRACTION24chain 'C' and (resid 25 through 87 )
25X-RAY DIFFRACTION25chain 'C' and (resid 88 through 101 )
26X-RAY DIFFRACTION26chain 'C' and (resid 102 through 131 )
27X-RAY DIFFRACTION27chain 'C' and (resid 132 through 143 )
28X-RAY DIFFRACTION28chain 'D' and (resid 3 through 23 )
29X-RAY DIFFRACTION29chain 'D' and (resid 24 through 45 )
30X-RAY DIFFRACTION30chain 'D' and (resid 46 through 77 )
31X-RAY DIFFRACTION31chain 'D' and (resid 78 through 101 )
32X-RAY DIFFRACTION32chain 'D' and (resid 102 through 123 )
33X-RAY DIFFRACTION33chain 'D' and (resid 124 through 140 )
34X-RAY DIFFRACTION34chain 'G' and (resid 1 through 5 )
35X-RAY DIFFRACTION35chain 'G' and (resid 6 through 10 )
36X-RAY DIFFRACTION36chain 'G' and (resid 11 through 15 )
37X-RAY DIFFRACTION37chain 'G' and (resid 16 through 18 )
38X-RAY DIFFRACTION38chain 'H' and (resid 20 through 24 )
39X-RAY DIFFRACTION39chain 'H' and (resid 25 through 29 )
40X-RAY DIFFRACTION40chain 'H' and (resid 30 through 34 )
41X-RAY DIFFRACTION41chain 'H' and (resid 35 through 37 )
42X-RAY DIFFRACTION42chain 'K' and (resid 148 through 193 )
43X-RAY DIFFRACTION43chain 'K' and (resid 194 through 208 )
44X-RAY DIFFRACTION44chain 'L' and (resid 149 through 164 )
45X-RAY DIFFRACTION45chain 'L' and (resid 165 through 178 )
46X-RAY DIFFRACTION46chain 'L' and (resid 179 through 209 )
47X-RAY DIFFRACTION47chain 'M' and (resid 150 through 163 )
48X-RAY DIFFRACTION48chain 'M' and (resid 164 through 178 )
49X-RAY DIFFRACTION49chain 'M' and (resid 179 through 207 )
50X-RAY DIFFRACTION50chain 'N' and (resid 149 through 164 )
51X-RAY DIFFRACTION51chain 'N' and (resid 165 through 178 )
52X-RAY DIFFRACTION52chain 'N' and (resid 179 through 193 )
53X-RAY DIFFRACTION53chain 'N' and (resid 194 through 209 )

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