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- PDB-6z71: Structure of the MATE family multidrug resistance transporter Aq_... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6z71 | ||||||
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Title | Structure of the MATE family multidrug resistance transporter Aq_128 from Aquifex aeolicus in the outward-facing state | ||||||
![]() | Aq128 | ||||||
![]() | MEMBRANE PROTEIN / MATE class transporter | ||||||
Function / homology | : / Multi antimicrobial extrusion protein / MatE / antiporter activity / xenobiotic transmembrane transporter activity / monoatomic ion transport / plasma membrane / Multidrug-efflux transporter![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhao, J. / Safarian, S. / Thielmann, Y. / Xie, H. / Wang, J. / Michel, H. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily. Authors: Zhao, J. / Xie, H. / Mehdipour, A.R. / Safarian, S. / Ermler, U. / Munke, C. / Thielmann, Y. / Hummer, G. / Ebersberger, I. / Wang, J. / Michel, H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 173.7 KB | Display | ![]() |
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PDB format | ![]() | 137.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.7 KB | Display | ![]() |
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Full document | ![]() | 458.9 KB | Display | |
Data in XML | ![]() | 30.4 KB | Display | |
Data in CIF | ![]() | 40.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6z70C ![]() 6fv6S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 55567.156 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: aq_128 / Cell line (production host): Top10 / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.98 % |
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Crystal grow | Temperature: 295 K / Method: lipidic cubic phase / pH: 6 Details: 0.1 M MES 0.1 M Magnesium chloride 0.1 M Sodium chloride 30% PEG 600 (v/v) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Apr 15, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.81517 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→19.978 Å / Num. obs: 14759 / % possible obs: 99.7 % / Redundancy: 3.5 % / CC1/2: 0.991 / Rmerge(I) obs: 0.178 / Rpim(I) all: 0.11 / Rrim(I) all: 0.21 / Net I/σ(I): 6.4 |
Reflection shell | Resolution: 3.5→3.62 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.84 / Mean I/σ(I) obs: 1.5 / Num. unique obs: 1453 / CC1/2: 0.618 / Rpim(I) all: 0.516 / Rrim(I) all: 0.989 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6FV6 Resolution: 3.5→19.978 Å / SU ML: 0.61 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 36.87 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 183.47 Å2 / Biso mean: 75.9581 Å2 / Biso min: 15.49 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.5→19.978 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 5
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