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- PDB-6z2m: H11-D4, SARS-CoV-2 RBD, CR3022 ternary complex -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 6z2m
TitleH11-D4, SARS-CoV-2 RBD, CR3022 ternary complex
Components
  • (CR3022 antibody) x 2
  • Spike glycoprotein
  • nanobody D4
KeywordsVIRAL PROTEIN / Antibody / nanobody complex / rbd
Function / homology
Function and homology information


Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / symbiont-mediated-mediated suppression of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / symbiont-mediated-mediated suppression of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell / membrane fusion / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / Attachment and Entry / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / host cell surface receptor binding / symbiont-mediated suppression of host innate immune response / receptor ligand activity / endocytosis involved in viral entry into host cell / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / identical protein binding / membrane / plasma membrane
Similarity search - Function
Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal / Spike glycoprotein, betacoronavirus / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus ...Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal / Spike glycoprotein, betacoronavirus / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2 / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Biological speciesSevere acute respiratory syndrome coronavirus 2
Homo sapiens (human)
Lama glama (llama)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.71 Å
AuthorsNaismith, J.H. / Ren, J. / Zhou, D. / Zhao, Y. / Stuart, D.I.
Funding support United Kingdom, 2items
OrganizationGrant numberCountry
Wellcome Trust100209/Z/12/Z United Kingdom
Engineering and Physical Sciences Research CouncilEP/S025243/1. United Kingdom
CitationJournal: To Be Published
Title: Structural characterisation of a nanobody derived from a naive library that neutralises SARS-CoV-2
Authors: Naismith, J.H.
History
DepositionMay 17, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jun 3, 2020Provider: repository / Type: Initial release
Revision 1.1Jul 29, 2020Group: Data collection / Derived calculations / Structure summary
Category: chem_comp / entity ...chem_comp / entity / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / struct_conn / struct_site / struct_site_gen
Item: _chem_comp.name / _entity.pdbx_description ..._chem_comp.name / _entity.pdbx_description / _pdbx_entity_nonpoly.name / _struct_conn.pdbx_role
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 1.2Jan 24, 2024Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.3Nov 6, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Spike glycoprotein
B: CR3022 antibody
C: CR3022 antibody
E: Spike glycoprotein
H: CR3022 antibody
L: CR3022 antibody
F: nanobody D4
D: nanobody D4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)166,90210
Polymers166,4608
Non-polymers4422
Water00
1
A: Spike glycoprotein
B: CR3022 antibody
C: CR3022 antibody
D: nanobody D4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)83,4515
Polymers83,2304
Non-polymers2211
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
E: Spike glycoprotein
H: CR3022 antibody
L: CR3022 antibody
F: nanobody D4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)83,4515
Polymers83,2304
Non-polymers2211
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)149.717, 150.395, 119.537
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP21212

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Components

#1: Protein Spike glycoprotein / S glycoprotein / E2 / Peplomer protein


Mass: 22115.834 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: S, 2 / Production host: Homo sapiens (human) / References: UniProt: P0DTC2
#2: Protein CR3022 antibody


Mass: 22874.785 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#3: Antibody CR3022 antibody


Mass: 24186.740 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#4: Antibody nanobody D4


Mass: 14052.671 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Lama glama (llama) / Production host: Escherichia coli (E. coli)
#5: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.05 Å3/Da / Density % sol: 69.62 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop
Details: Purified RBD, Fab CR3022 and nanobody H11-D4 were mixed together at a molar ratio of 1:1:1 to a final concentration of approximately 7 mg/ml and incubated at room temperature for one hour. ...Details: Purified RBD, Fab CR3022 and nanobody H11-D4 were mixed together at a molar ratio of 1:1:1 to a final concentration of approximately 7 mg/ml and incubated at room temperature for one hour. Initial screening was performed in 96-well plates using the nanolitre sitting-drop vapour diffusion method. The best crystals were grown in condition containing 0.1 M sodium citrate tribasic dihydrate, pH 5.0, 10% (w/v) Polyethylene glycol 6000.

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.97625 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 10, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97625 Å / Relative weight: 1
ReflectionResolution: 2.71→106.1 Å / Num. obs: 73071 / % possible obs: 99 % / Redundancy: 13.2 % / CC1/2: 0.997 / Rmerge(I) obs: 0.231 / Rpim(I) all: 0.065 / Net I/σ(I): 7.4
Reflection shellResolution: 2.71→2.76 Å / Redundancy: 9 % / Mean I/σ(I) obs: 0.2 / Num. unique obs: 3426 / CC1/2: 0.087 / Rpim(I) all: 3.953 / % possible all: 94

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
XDSdata reduction
PHASERphasing
PHENIX1.17.1_3660refinement
PDB_EXTRACT3.25data extraction
XSCALEdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6YZ7

6yz7


Resolution: 2.71→74.86 Å / SU ML: 0.26 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 26.16 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.241 2365 4.99 %
Rwork0.1983 45047 -
obs0.2004 47412 64.33 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 213.92 Å2 / Biso mean: 53.5819 Å2 / Biso min: 11.46 Å2
Refinement stepCycle: final / Resolution: 2.71→74.86 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11699 0 28 0 11727
Biso mean--70.23 --
Num. residues----1516
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 17

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.71-2.770.289350.2988951002
2.77-2.830.4119360.379747150712
2.83-2.890.3867410.336276180219
2.89-2.970.3558470.3204997104424
2.97-3.050.3781420.30771264130630
3.05-3.140.3256950.29161544163938
3.14-3.240.29891170.27541985210249
3.24-3.350.29771260.26192480260661
3.35-3.490.32561590.25052953311272
3.49-3.650.26622000.22183376357683
3.65-3.840.29121720.20764015418797
3.84-4.080.25472140.190841194333100
4.08-4.390.22032100.164141104320100
4.39-4.840.16872380.147341354373100
4.84-5.530.19131860.165941984384100
5.54-6.970.23342460.200741854431100
6.97-74.860.22292310.19054359459099
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.83970.8253-0.66432.35760.92284.4238-0.09270.38780.7183-0.44720.086-0.6446-0.90610.0734-0.24430.40110.04080.13670.65560.15720.491712.709748.040649.9923
23.14841.0326-2.42826.5753-2.40584.85790.0805-0.17111.206-0.26740.0385-0.14320.0440.1141-0.11640.12460.080.14190.48310.04180.504123.167139.156645.3228
35.8064-1.6961-1.63450.82350.77380.72590.9291.62291.0128-0.7020.03180.8049-0.7377-0.9859-0.15870.57730.32-0.02761.03380.33250.476514.799339.587435.1974
42.76740.5962-1.32442.44590.18923.6668-0.16650.8275-0.4068-0.16290.1942-0.21770.5458-0.1973-0.0560.2561-0.01980.06910.3491-0.05990.340513.186231.841256.6216
53.6950.0429-1.00691.9984-0.28993.9439-0.0308-0.0677-0.15220.08650.1147-0.06620.07160.0703-0.07250.1545-0.02780.03950.2966-0.02150.263910.847134.302864.5598
61.4141-3.26470.51777.6473-0.20968.84110.41471.9086-0.3169-1.3042-0.01370.5983-0.14470.1023-0.01430.6725-0.2246-0.00641.19130.14050.82767.425443.595634.2127
71.4611-0.43850.08061.53710.56791.37130.2186-0.4182-0.2954-0.001-0.0513-0.22570.2996-0.3478-0.09530.2064-0.04930.02070.16780.05820.263436.152724.044737.6237
81.4667-0.14180.22440.6552-1.02411.5752-0.0711-0.1403-0.49250.2633-0.03310.25830.9238-0.5176-0.05940.3846-0.0685-0.00760.23710.030.293827.889817.012433.1507
92.23850.35780.47111.7470.22190.14260.0591-0.4407-0.7970.43580.03140.39940.6234-0.3546-0.04930.3928-0.4510.0770.25490.5830.238727.434317.0244.2507
102.01990.2170.14071.60540.98671.5029-0.0415-0.4701-0.41660.04910.18970.32250.4393-0.21790.00580.4046-0.0152-0.03990.13480.06560.267535.506518.032938.5573
111.2872-0.6513-0.29761.59180.68612.0005-0.067-0.01820.0678-0.23930.5021-0.26720.4283-0.0618-0.21730.3473-0.21280.01860.1485-0.03240.323143.825515.712618.3452
123.13541.1752-1.64534.7179-4.00223.5471-0.2033-0.28850.50230.01150.2589-0.2863-1.30060.41690.19060.7219-0.1564-0.01270.2622-0.19110.568550.668915.570116.5341
133.08510.4378-0.21460.1682-0.34072.0091-0.1032-0.15580.01790.01950.12010.1416-0.77110.1464-0.11980.6099-0.0019-0.1280.3588-0.06990.433545.277412.283212.5509
141.7662-0.4605-0.66361.668-1.18621.4296-0.0574-0.21041.1345-0.03720.2365-0.6898-0.67690.9171-0.00710.8883-0.3264-0.07140.5297-0.04640.619254.834718.64694.1383
152.1593-0.1366-0.53982.0881-0.68320.3793-0.4283-0.22410.2417-0.38460.5553-0.6924-0.62650.909-0.14720.6595-0.3164-0.06260.7275-0.24080.64459.082713.690811.9532
162.65610.1841-1.47452.49380.28015.00830.0861-0.084-0.0014-0.3035-0.03980.74760.5596-0.7491-0.09250.289-0.0149-0.23720.4211-0.02020.636413.384822.30821.766
171.3913-1.3564-1.33582.16551.77472.47490.33350.0172-0.1766-0.4145-0.15820.1839-0.2152-0.4092-0.11570.2946-0.0524-0.14310.28350.03570.282824.485923.91217.2897
181.7878-0.8647-0.58373.17781.19192.2266-0.2551-0.1563-0.0863-0.34530.16560.0133-0.3325-0.00590.0230.576-0.0719-0.04640.2393-0.12580.339242.77833.92080.9943
195.26050.5910.52973.1085-0.77263.746-0.0256-0.5386-0.3747-0.3830.19471.10430.2335-0.7394-0.01030.4005-0.0869-0.09620.35980.11880.580923.048551.14363.8263
203.922-0.3828-2.65482.5299-0.17453.14430.14-0.1273-0.31310.20810.17410.9499-0.3421-0.3905-0.47990.5345-0.03820.34010.1530.47150.173427.09957.85355.6529
211.13350.2976-0.7780.32420.28141.5067-0.0114-0.0364-0.20320.0380.34320.82710.1624-0.31940.67870.49390.0559-0.15530.20320.29040.65129.0742.463412.1205
222.0919-0.83731.98295.4804-1.23311.9116-0.6326-0.67490.00750.75130.3310.8706-0.6112-0.3177-0.18120.6240.12440.10980.29010.00720.444233.196751.497118.1742
232.97710.33980.07294.67940.80734.28220.04910.2421-0.40680.43770.0186-0.2828-0.34490.3982-0.17250.2785-0.01520.00550.06580.04950.17937.892651.89331.6912
242.66030.0313-0.8373.8497-0.65613.333-0.04970.1066-0.1639-0.03470.3291-0.10370.03980.0379-0.13670.2233-0.0656-0.00370.11370.03520.25739.114550.7598-1.1796
253.0506-0.10180.25212.4129-0.15493.33160.20070.32390.1434-0.5945-0.0908-0.3283-0.65750.4151-0.12840.5289-0.02560.08410.2348-0.050.199644.132659.0329-12.3592
263.076-1.6364-1.55345.69532.83265.7818-0.0886-0.31070.58960.70810.29410.2706-0.7577-0.0812-0.03350.6782-0.09020.01310.00360.06580.4430.041256.648312.1121
272.44970.30760.92191.9983-0.18711.48170.1217-0.0071-0.3097-0.20940.24020.05160.20190.047-0.1370.20240.02870.00410.1041-0.03610.148445.762830.942222.9209
282.5283-1.63310.13642.1789-0.06222.06910.09140.19460.2576-0.17180.013-0.3109-0.0540.2755-0.16050.18140.01140.04540.1003-0.00950.172553.453638.256421.7916
291.5378-0.23460.34741.0767-0.78731.73280.147-0.0571-0.05790.4785-0.12460.0557-0.27930.2146-0.03770.2974-0.010.03540.0362-0.00250.250551.244231.289438.2845
302.0584-1.06370.45832.01920.28093.51560.1298-0.1641-0.3185-0.0006-0.04330.47390.5811-0.3188-0.02910.2538-0.0289-0.00380.31720.12970.487952.761520.835755.0345
311.7765-0.13430.03731.2692-0.58014.0934-0.3361-0.36910.7133-0.17790.07880.1971-0.78010.0796-0.44620.55030.0187-0.09030.021-0.15110.473945.713757.309636.458
322.87170.02140.13253.5491-0.97623.8979-0.08210.03580.02670.33650.1070.2816-0.4606-0.423-0.05440.24590.08260.03010.2072-0.03370.123938.262447.45236.0181
331.7786-0.23441.05180.7041-0.84672.4375-0.0436-0.24510.18850.0548-0.0388-0.0824-0.417-0.10470.10920.35410.05170.01940.2117-0.09640.231244.504750.820841.7827
346.3274-3.1117-0.02083.41471.31271.85130.5977-0.1367-0.3162-0.188-0.3878-0.21050.1670.1267-0.1550.3193-0.0595-0.02750.25590.020.281259.772728.569561.665
353.2741-0.86593.72040.4012-1.00354.22740.1784-0.21410.4811-0.0377-0.298-0.5301-0.39230.6867-0.06920.22930.0037-0.07160.47150.0380.399269.447536.784265.205
361.8725-2.0277-0.19052.2827-0.25342.51741.13770.3012-0.2674-0.2587-0.77050.1301-0.056-0.1067-0.27630.348-0.0478-0.00270.3146-0.01580.421352.837936.786255.8665
374.0997-2.14630.34833.0851-0.19192.411-0.2267-0.7276-0.1980.63420.2121-0.2703-0.0580.281-0.14210.30570.0382-0.05550.41330.0180.338765.650931.501665.9058
383.31380.09410.87282.64931.05555.0222-0.0806-0.17790.4114-0.2287-0.0010.409-0.3516-2.3753-0.08450.59310.3126-0.0781.01910.01210.44528.401954.5267-36.027
392.35420.9813-0.32324.75443.82493.682-0.33540.1496-0.90570.3142-0.12910.22820.18710.03080.21920.48930.21230.03670.8208-0.13520.230639.584650.3061-36.9406
401.41920.8781.231.4259-0.56053.0471-0.02980.4480.0279-0.1269-0.0050.113-1.2633-0.9304-0.03820.75260.33980.02390.81150.00170.233936.446658.0494-37.2396
412.64870.4554-0.40231.34990.11386.87180.20270.0808-0.3159-0.13620.1643-0.1980.4524-0.8955-0.14250.38450.15570.01580.5535-0.05760.247137.993350.4158-31.6518
425.4668-0.337-3.71793.4890.71666.9115-0.6979-0.40390.55540.36510.2408-0.3892-0.5073-0.36760.4640.5491-0.0178-0.05270.8333-0.4070.7626.283652.987294.4418
434.60530.30481.97615.66923.13772.7834-0.48770.05540.9732-0.6113-0.5395-0.7721-1.40870.73080.7370.35940.0032-0.06380.62890.00990.510210.697846.631284.4724
444.9526-0.08031.14464.6705-0.16454.2825-0.3285-0.77820.11740.5912-0.0027-0.3103-0.21580.13170.37330.24910.0356-0.01070.6267-0.0360.36274.893842.707493.2615
450.351-0.5874-0.29511.0340.93683.0345-0.0232-0.54070.18550.28410.3543-0.3247-0.50460.9746-0.23910.249-0.08760.0520.9169-0.13450.386612.538943.351388.9513
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 332 through 364 )A332 - 364
2X-RAY DIFFRACTION2chain 'A' and (resid 365 through 380 )A365 - 380
3X-RAY DIFFRACTION3chain 'A' and (resid 381 through 393 )A381 - 393
4X-RAY DIFFRACTION4chain 'A' and (resid 394 through 421 )A394 - 421
5X-RAY DIFFRACTION5chain 'A' and (resid 422 through 516 )A422 - 516
6X-RAY DIFFRACTION6chain 'A' and (resid 517 through 528 )A517 - 528
7X-RAY DIFFRACTION7chain 'B' and (resid 0 through 39 )B0 - 39
8X-RAY DIFFRACTION8chain 'B' and (resid 40 through 52 )B40 - 52
9X-RAY DIFFRACTION9chain 'B' and (resid 53 through 73 )B53 - 73
10X-RAY DIFFRACTION10chain 'B' and (resid 74 through 100 )B74 - 100
11X-RAY DIFFRACTION11chain 'B' and (resid 101 through 147 )B101 - 147
12X-RAY DIFFRACTION12chain 'B' and (resid 148 through 161 )B148 - 161
13X-RAY DIFFRACTION13chain 'B' and (resid 162 through 181 )B162 - 181
14X-RAY DIFFRACTION14chain 'B' and (resid 182 through 204 )B182 - 204
15X-RAY DIFFRACTION15chain 'B' and (resid 205 through 219 )B205 - 219
16X-RAY DIFFRACTION16chain 'C' and (resid 1 through 38 )C1 - 38
17X-RAY DIFFRACTION17chain 'C' and (resid 39 through 128 )C39 - 128
18X-RAY DIFFRACTION18chain 'C' and (resid 129 through 219 )C129 - 219
19X-RAY DIFFRACTION19chain 'E' and (resid 333 through 349 )E333 - 349
20X-RAY DIFFRACTION20chain 'E' and (resid 350 through 364 )E350 - 364
21X-RAY DIFFRACTION21chain 'E' and (resid 365 through 375 )E365 - 375
22X-RAY DIFFRACTION22chain 'E' and (resid 376 through 393 )E376 - 393
23X-RAY DIFFRACTION23chain 'E' and (resid 394 through 409 )E394 - 409
24X-RAY DIFFRACTION24chain 'E' and (resid 410 through 451 )E410 - 451
25X-RAY DIFFRACTION25chain 'E' and (resid 452 through 506 )E452 - 506
26X-RAY DIFFRACTION26chain 'E' and (resid 507 through 527 )E507 - 527
27X-RAY DIFFRACTION27chain 'H' and (resid 0 through 32 )H0 - 32
28X-RAY DIFFRACTION28chain 'H' and (resid 33 through 91 )H33 - 91
29X-RAY DIFFRACTION29chain 'H' and (resid 92 through 138 )H92 - 138
30X-RAY DIFFRACTION30chain 'H' and (resid 139 through 219 )H139 - 219
31X-RAY DIFFRACTION31chain 'L' and (resid 1 through 38 )L1 - 38
32X-RAY DIFFRACTION32chain 'L' and (resid 39 through 67 )L39 - 67
33X-RAY DIFFRACTION33chain 'L' and (resid 68 through 119 )L68 - 119
34X-RAY DIFFRACTION34chain 'L' and (resid 120 through 149 )L120 - 149
35X-RAY DIFFRACTION35chain 'L' and (resid 150 through 161 )L150 - 161
36X-RAY DIFFRACTION36chain 'L' and (resid 162 through 178 )L162 - 178
37X-RAY DIFFRACTION37chain 'L' and (resid 179 through 219 )L179 - 219
38X-RAY DIFFRACTION38chain 'F' and (resid 1 through 32 )F1 - 32
39X-RAY DIFFRACTION39chain 'F' and (resid 33 through 52 )F33 - 52
40X-RAY DIFFRACTION40chain 'F' and (resid 53 through 99 )F53 - 99
41X-RAY DIFFRACTION41chain 'F' and (resid 100 through 127 )F100 - 127
42X-RAY DIFFRACTION42chain 'D' and (resid 1 through 25 )D1 - 25
43X-RAY DIFFRACTION43chain 'D' and (resid 26 through 39 )D26 - 39
44X-RAY DIFFRACTION44chain 'D' and (resid 40 through 91 )D40 - 91
45X-RAY DIFFRACTION45chain 'D' and (resid 92 through 127 )D92 - 127

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