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Yorodumi- PDB-6yxp: Higher resolution structure of the N-terminal module of the human... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6yxp | ||||||
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Title | Higher resolution structure of the N-terminal module of the human SWI/SNF-subunit BAF155/SMARCC1 | ||||||
Components | SWI/SNF complex subunit SMARCC1 | ||||||
Keywords | SIGNALING PROTEIN / Baf155 / SWI/SNF-subunit | ||||||
Function / homology | Function and homology information prostate gland development / npBAF complex / brahma complex / nBAF complex / GBAF complex / regulation of G0 to G1 transition / nucleosome disassembly / regulation of nucleotide-excision repair / XY body / RSC-type complex ...prostate gland development / npBAF complex / brahma complex / nBAF complex / GBAF complex / regulation of G0 to G1 transition / nucleosome disassembly / regulation of nucleotide-excision repair / XY body / RSC-type complex / regulation of mitotic metaphase/anaphase transition / SWI/SNF complex / positive regulation of double-strand break repair / positive regulation of T cell differentiation / positive regulation of stem cell population maintenance / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known / regulation of G1/S transition of mitotic cell cycle / negative regulation of cell differentiation / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / positive regulation of myoblast differentiation / nucleosomal DNA binding / male germ cell nucleus / positive regulation of cell differentiation / animal organ morphogenesis / kinetochore / RMTs methylate histone arginines / nuclear matrix / insulin receptor signaling pathway / nervous system development / histone binding / transcription coactivator activity / chromatin remodeling / positive regulation of cell population proliferation / chromatin / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / protein-containing complex / nucleoplasm / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.6 Å | ||||||
Authors | Allen, M.D. / Bycroft, M. / Zinzalla, G. | ||||||
Citation | Journal: Commun Biol / Year: 2021 Title: SWI/SNF subunit BAF155 N-terminus structure informs the impact of cancer-associated mutations and reveals a potential drug binding site. Authors: Allen, M.D. / Freund, S.M.V. / Bycroft, M. / Zinzalla, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6yxp.cif.gz | 243.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6yxp.ent.gz | 196.4 KB | Display | PDB format |
PDBx/mmJSON format | 6yxp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6yxp_validation.pdf.gz | 430.2 KB | Display | wwPDB validaton report |
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Full document | 6yxp_full_validation.pdf.gz | 435.2 KB | Display | |
Data in XML | 6yxp_validation.xml.gz | 26.1 KB | Display | |
Data in CIF | 6yxp_validation.cif.gz | 39.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yx/6yxp ftp://data.pdbj.org/pub/pdb/validation_reports/yx/6yxp | HTTPS FTP |
-Related structure data
Related structure data | 6yxoSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 32854.195 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SMARCC1, BAF155 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): C41 / References: UniProt: Q92922 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 49.77 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, sitting drop / pH: 5.9 Details: 0.2 M Magnesium formate (pH 5.9), 20% PEG 3350. Protein at 16 mg/ml. |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.939273 Å |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: May 23, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.939273 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→49.49 Å / Num. obs: 82320 / % possible obs: 99.1 % / Redundancy: 6.8 % / CC1/2: 0.999 / Rmerge(I) obs: 0.053 / Rpim(I) all: 0.022 / Rrim(I) all: 0.057 / Net I/σ(I): 15.1 |
Reflection shell | Resolution: 1.6→1.69 Å / Redundancy: 6.9 % / Rmerge(I) obs: 0.611 / Mean I/σ(I) obs: 2.7 / Num. unique obs: 11930 / CC1/2: 0.85 / Rpim(I) all: 0.248 / Rrim(I) all: 0.661 / % possible all: 98.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6YXO Resolution: 1.6→42.066 Å / SU ML: 0.21 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 21.1
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→42.066 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -12.2151 Å / Origin y: 50.3313 Å / Origin z: 67.6816 Å
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Refinement TLS group | Selection details: all |