分子量: 16806.496 Da / 分子数: 2 / 由来タイプ: 組換発現 詳細: The expressed protein comprised residues 111-242 of the wild-type sequence with an additional N-terminal Met residue and a C-terminal nickel affinity tag of sequence KLAAALEHHHHHH from the ...詳細: The expressed protein comprised residues 111-242 of the wild-type sequence with an additional N-terminal Met residue and a C-terminal nickel affinity tag of sequence KLAAALEHHHHHH from the pET21b expression plasmid 由来: (組換発現) Bacillus subtilis (strain 168) (枯草菌) 遺伝子: noc, yyaA, BSU40990 / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): Rosetta / Variant (発現宿主): Solu / 参照: UniProt: P37524
#2: DNA鎖
NocBindingSite (NBS)
分子量: 6751.378 Da / 分子数: 2 / 由来タイプ: 合成 詳細: Chains C and D form a symmetrical DNA duplex which is similar to the Noc Binding Site (NBS) sequence from Bacillus subtilis without 5' phosphates 由来: (合成) synthetic construct (人工物)
解像度: 2.23→72.3 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.921 / SU ML: 0.385 / SU R Cruickshank DPI: 0.3856 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R Free: 0.441 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES: WITH TLS ADDED This structure was refined after applying an anisotropic correction to the data with STARANISO. In line with the ...詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES: WITH TLS ADDED This structure was refined after applying an anisotropic correction to the data with STARANISO. In line with the recommendations of the PDB and the STARANISO webpages, the uploaded data cif file contains three data blocks: 1. the STARANISO corrected data that was used to refine the structure, 2. the full uncorrected dataset with no resolution cut-off applied, 3. the output from refmac5 with filled-in data removed to leave only the reflections that were observed within the ellipsoidal resolution cut-off. The latter contains blurred map coefficients that should enable reproduction of the maps that were used to evaluate the model. N.B. The anisotropic correction leads to poor completeness in the higher spherical resolution shells resulting in maps that appear to be at lower resolution than the 2.23 Ang maximum resolution of the corrected data. Since the Rfree and RSRZ scores which evaluate the fit of the model to the data are dependent on the maximum reported resolution, they are inevitably poor for this model. Also note that the map coefficients automatically generated by the deposition process have unfortunately re-instated the above mentioned filled-in reflections. Thus, these will produce maps with an element of model bias i.e. they will tend to reproduce the model.