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Yorodumi- PDB-6y4h: Solution structure of cold-shock domain 7 and 8 of drosophila Ups... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6y4h | ||||||
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Title | Solution structure of cold-shock domain 7 and 8 of drosophila Upstream of N-Ras (Unr) | ||||||
Components | Upstream of N-ras, isoform A | ||||||
Keywords | RNA BINDING PROTEIN / CSD / ncCSD | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Hollmann, N.M. / Simon, B. / Hennig, J. | ||||||
Citation | Journal: Cell Rep / Year: 2020 Title: Pseudo-RNA-Binding Domains Mediate RNA Structure Specificity in Upstream of N-Ras. Authors: Hollmann, N.M. / Jagtap, P.K.A. / Masiewicz, P. / Guitart, T. / Simon, B. / Provaznik, J. / Stein, F. / Haberkant, P. / Sweetapple, L.J. / Villacorta, L. / Mooijman, D. / Benes, V. / ...Authors: Hollmann, N.M. / Jagtap, P.K.A. / Masiewicz, P. / Guitart, T. / Simon, B. / Provaznik, J. / Stein, F. / Haberkant, P. / Sweetapple, L.J. / Villacorta, L. / Mooijman, D. / Benes, V. / Savitski, M.M. / Gebauer, F. / Hennig, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6y4h.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb6y4h.ent.gz | 963.1 KB | Display | PDB format |
PDBx/mmJSON format | 6y4h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/y4/6y4h ftp://data.pdbj.org/pub/pdb/validation_reports/y4/6y4h | HTTPS FTP |
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-Related structure data
Related structure data | 6y6eC 6y6mC 6y96C C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 18672.947 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) Gene: Unr, BcDNA:LD13080, CR32028, Dmel\CG7015, dUNR, MRE30, UNR, unr, CG7015, Dmel_CG7015 Plasmid: pETM11 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q9VSK3 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 150 mM / Label: conditions_1 / pH: 6.4 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 5 | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |