Crystal structure of Staphylococcal nuclease variant Delta+PHS F34Y at cryogenic temperature
要素
Thermonuclease
キーワード
HYDROLASE / nuclease / polar group / pdTp
機能・相同性
機能・相同性情報
endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters / micrococcal nuclease / nucleic acid binding / extracellular region / membrane / metal ion binding 類似検索 - 分子機能
Thermonuclease family signature 1. / Thermonuclease active site / Thermonuclease family signature 2. / Staphylococcal nuclease (SNase-like), OB-fold / Staphylococcal nuclease homologue / Thermonuclease domain profile. / Staphylococcal nuclease homologues / SNase-like, OB-fold superfamily 類似検索 - ドメイン・相同性
解像度: 2.1→24.63 Å / Cor.coef. Fo:Fc: 0.939 / Cor.coef. Fo:Fc free: 0.899 / WRfactor Rfree: 0.2342 / WRfactor Rwork: 0.1986 / FOM work R set: 0.7041 / SU B: 9.209 / SU ML: 0.229 / SU R Cruickshank DPI: 0.2706 / SU Rfree: 0.2205 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.271 / ESU R Free: 0.221 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
Rfactor
反射数
%反射
Selection details
Rfree
0.2791
417
5 %
RANDOM
Rwork
0.2306
-
-
-
obs
0.2331
7937
99.94 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK