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- PDB-6xet: Tubulin-RB3_SLD in complex with compound 60c -

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Basic information

Entry
Database: PDB / ID: 6xet
TitleTubulin-RB3_SLD in complex with compound 60c
Components
  • Stathmin-4
  • Tubulin alpha-1B chain
  • Tubulin beta chain
KeywordsCELL CYCLE/INHIBITOR / MICROTUBULE INHIBITOR / COLCHICINE / CELL CYCLE / CANCER / CELL CYCLE-INHIBITOR COMPLEX
Function / homology
Function and homology information


Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Hedgehog 'off' state / Cilium Assembly / Intraflagellar transport / COPI-dependent Golgi-to-ER retrograde traffic / Carboxyterminal post-translational modifications of tubulin / RHOH GTPase cycle / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / PKR-mediated signaling ...Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Hedgehog 'off' state / Cilium Assembly / Intraflagellar transport / COPI-dependent Golgi-to-ER retrograde traffic / Carboxyterminal post-translational modifications of tubulin / RHOH GTPase cycle / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / PKR-mediated signaling / The role of GTSE1 in G2/M progression after G2 checkpoint / Aggrephagy / Resolution of Sister Chromatid Cohesion / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Separation of Sister Chromatids / RHO GTPases activate IQGAPs / RHO GTPases Activate Formins / Recruitment of NuMA to mitotic centrosomes / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / microtubule depolymerization / COPI-mediated anterograde transport / regulation of microtubule polymerization or depolymerization / tubulin binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / structural constituent of cytoskeleton / microtubule cytoskeleton organization / neuron projection development / microtubule cytoskeleton / mitotic cell cycle / growth cone / microtubule / neuron projection / GTPase activity / GTP binding / Golgi apparatus / metal ion binding / cytoplasm
Similarity search - Function
Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site ...Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily
Similarity search - Domain/homology
GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Chem-TU2 / Tubulin beta chain / Stathmin-4 / Tubulin alpha-1B chain
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
Sus scrofa (pig)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å
AuthorsWhite, S.W. / Yun, M.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Cancer Institute (NIH/NCI)R01CA148706 United States
CitationJournal: J.Med.Chem. / Year: 2021
Title: Design, Synthesis, and Biological Evaluation of Stable Colchicine-Binding Site Tubulin Inhibitors 6-Aryl-2-benzoyl-pyridines as Potential Anticancer Agents.
Authors: Chen, H. / Deng, S. / Albadari, N. / Yun, M.K. / Zhang, S. / Li, Y. / Ma, D. / Parke, D.N. / Yang, L. / Seagroves, T.N. / White, S.W. / Miller, D.D. / Li, W.
History
DepositionJun 13, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 25, 2021Provider: repository / Type: Initial release
Revision 1.1Sep 8, 2021Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.2Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Tubulin alpha-1B chain
B: Tubulin beta chain
C: Tubulin alpha-1B chain
D: Tubulin beta chain
E: Stathmin-4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)214,82915
Polymers211,7095
Non-polymers3,12010
Water2,522140
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area19640 Å2
ΔGint-143 kcal/mol
Surface area65360 Å2
MethodPISA
Unit cell
Length a, b, c (Å)66.097, 126.938, 250.461
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

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Components

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Protein , 3 types, 5 molecules ACBDE

#1: Protein Tubulin alpha-1B chain / Alpha-tubulin ubiquitous / Tubulin K-alpha-1 / Tubulin alpha-ubiquitous chain


Mass: 48780.117 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: Q2XVP4
#2: Protein Tubulin beta chain


Mass: 48648.652 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A287AGU7
#3: Protein Stathmin-4 / Stathmin-like protein B3 / RB3


Mass: 16851.133 Da / Num. of mol.: 1 / Mutation: C14A, F20W
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Stmn4 / Production host: Escherichia coli (E. coli) / References: UniProt: P63043

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Non-polymers , 6 types, 150 molecules

#4: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#5: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: SO4
#6: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#7: Chemical ChemComp-TU2 / [3-fluoro-6-(3-hydroxy-4-methylphenyl)pyridin-2-yl](3,4,5-trimethoxyphenyl)methanone


Mass: 397.396 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C22H20FNO5 / Feature type: SUBJECT OF INVESTIGATION
#8: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg
#9: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 140 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.48 Å3/Da / Density % sol: 50.43 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.6 / Details: PEG 4000, ammonium sulfate, tri-sodium citrate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-BM / Wavelength: 1 Å
DetectorType: RAYONIX MX300-HS / Detector: CCD / Date: Nov 21, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.6→50 Å / Num. obs: 65688 / % possible obs: 99.3 % / Redundancy: 5.8 % / Biso Wilson estimate: 46.9 Å2 / CC1/2: 0.995 / Rmerge(I) obs: 0.112 / Rpim(I) all: 0.051 / Net I/σ(I): 15.5
Reflection shellResolution: 2.6→2.66 Å / Redundancy: 5.2 % / Rmerge(I) obs: 0.564 / Mean I/σ(I) obs: 1.8 / Num. unique obs: 3800 / CC1/2: 0.812 / Rpim(I) all: 0.268 / % possible all: 92.7

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Processing

Software
NameVersionClassification
PHENIX1.14_3260refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5XIW

5xiw


Resolution: 2.6→45.46 Å / SU ML: 0.3103 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 25.014 / Stereochemistry target values: CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2307 1993 3.05 %
Rwork0.1682 63372 -
obs0.1701 65365 99.28 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 58.33 Å2
Refinement stepCycle: LAST / Resolution: 2.6→45.46 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms14446 0 198 140 14784
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.007514968
X-RAY DIFFRACTIONf_angle_d0.928320300
X-RAY DIFFRACTIONf_chiral_restr0.05152206
X-RAY DIFFRACTIONf_plane_restr0.00542637
X-RAY DIFFRACTIONf_dihedral_angle_d18.75168943
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.6-2.670.34381320.25044197X-RAY DIFFRACTION93.32
2.67-2.740.32071370.24644375X-RAY DIFFRACTION97.62
2.74-2.820.32661420.23444491X-RAY DIFFRACTION99.81
2.82-2.910.2961410.21664501X-RAY DIFFRACTION100
2.91-3.010.26951430.21464530X-RAY DIFFRACTION100
3.01-3.130.28531410.20474498X-RAY DIFFRACTION99.98
3.13-3.280.25871420.19144516X-RAY DIFFRACTION99.91
3.28-3.450.24671430.17874522X-RAY DIFFRACTION100
3.45-3.660.23581430.16374545X-RAY DIFFRACTION100
3.66-3.950.21131420.14734543X-RAY DIFFRACTION100
3.95-4.340.19121430.13544572X-RAY DIFFRACTION99.94
4.34-4.970.19411460.12354589X-RAY DIFFRACTION99.83
4.97-6.260.21391450.16394655X-RAY DIFFRACTION100
6.26-45.460.1961530.15674838X-RAY DIFFRACTION99.48
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.902362390361.48792585145-0.6514082095382.9852746711-0.191615824154.347960746020.0588881720452-0.458694991712-0.1818578776560.378758054549-0.1446232658970.3003270038530.228732901744-0.1564281653570.1348541652830.539275794923-0.01035614502520.02547690419630.366518874267-0.005395901033230.372231180051-17.6952337956-28.01702217865.0747936094
25.188112280741.69075295729-1.573392853957.38869654822-2.692839112024.039749997470.334198203643-0.3005416349280.6015333993790.3200885750770.1640715784670.777554983986-1.25888679043-0.440761281337-0.3359341841440.7661619215190.07997271998350.08587529714160.463322088185-0.02123082240990.388540272694-13.7602356876-15.612833571762.114374032
32.269491704570.944306166163-1.572724324473.37878420066-1.063858012132.8589156458-0.00212484577264-0.317119117451-0.1257024863340.292058213425-0.0918926657628-0.2083116719060.004355822338810.2421975512950.08480003122460.430502551796-0.000382960144877-0.03703491858960.3915381828880.06123433909020.301918426432-0.262761663931-24.019440086354.8414548102
43.22955219495-0.11005550195-0.4621152504975.06671897956-0.3786995171772.62023346303-0.2161974519190.2554649363610.159182422038-0.162057753736-0.02114323226930.08999206923190.272780585289-0.2276810676530.2382278170430.471414879162-0.0517072235555-0.006946632215610.369660682839-0.02524202392920.350195833221-16.6363777599-32.702172994948.7443934577
52.901518839271.574447728240.2247877052273.082241866330.004244454538283.53599886625-0.2934577817070.469166766844-0.14887396633-0.4616472410120.156327254440.4430791938460.31593975273-0.412498257770.1368080799250.542502100067-0.0875157347288-0.02097114233440.4032843445780.01561940559410.371729629974-17.9554583333-39.486397694242.5371792846
64.778641319580.111872206246-0.9138941129643.814911887570.2072616923182.49229089188-0.006468125145950.199730864416-0.515496898437-0.69614011312-0.01869382615370.3007109990920.888061280741-0.3247155856990.1060478845560.868093796556-0.103910175357-0.05504323129740.4212204744720.06485381127990.558567980503-19.0061981049-49.743105105243.7949217415
73.53412308624-1.35509763026-0.3940387885465.958165334670.1275876759462.89153955289-0.140596099415-0.205206821201-0.493017972572-0.2516077657280.2150570985060.3478378387740.572663523249-0.01632103902920.01694816001160.531861351823-0.082434841731-0.03098965669910.3984345861710.03070326631320.345196113914-18.8767121971-43.584503380747.701254577
81.692306336291.29491931261-0.2453947856466.0513284972-0.372518636172.110468528-0.1532228122650.0358818449403-0.304264950896-0.180896362722-0.12030723364-0.6359065357750.4153985363980.1997778285940.2389668193850.4848071897910.04887529937530.09363279979530.4253432806410.07531396731530.3935179753896.24188319655-27.539465139837.9089705563
96.213160127560.931605384023-0.1679807027292.98025064973-1.049274172612.655676145110.282131865278-0.4600174641660.6123014597130.644594854959-0.09935836950110.785582981519-0.456672272159-0.383375904317-0.2163866498060.6868236343470.04053885277260.1258464773120.396616121121-0.1024076966770.533595689061-10.656585490314.120717518846.2245122412
103.492394272010.824917263709-2.477671045612.677071144170.138336338274.505704588560.387205651783-0.65663844580.4410939914620.837918926493-0.2870221433320.318150734512-0.3802086581080.316401391099-0.1052645358470.703813260383-0.126405543148-0.008632117606190.474524433716-0.08678924348680.4442786363497.9425260485119.996516014445.4277158836
112.517288363911.44821722682-0.1807542262762.948747909350.2373692209191.039917090130.004225823099340.002090647577850.2043362156060.12691493142-0.001485024738260.126016843759-0.212371099275-0.0130871964283-0.01912243857170.4342319964470.0356512834436-0.005343539385940.3013632886930.02520458838220.3221802148521.1586216385510.606117955932.8158481184
125.24886296519-2.89830597685-0.6926292606583.581356236761.025264128152.43403281786-0.181050175669-0.09883775543610.1066521371430.207268130320.007488648602380.113098114170.124663055371-0.1128812225570.1540005950040.434343989648-0.122433992738-0.03042100401040.272790883109-0.003483925505720.241132463158-2.85023150192-4.3660917658239.2106587874
133.039645568391.07804650602-0.258506796121.36006345911-0.8712205132352.27022060747-0.1272605415980.1715725739670.194733320371-0.3098668734810.06505657607950.69132245870.0226188426808-0.4414544122910.007812805141290.491793338079-0.0764518883618-0.1021210520280.5219549964880.05737225014890.622452903082-11.60244436190.38660629127422.3989890219
144.43989540918-1.0558932075-0.7475568521834.281650175650.5861888462752.14469020284-0.208943706870.257740550352-0.246726377128-0.1878887673720.144297240730.707624272580.231327175932-0.482983132590.08303280555540.460280857809-0.0983155048737-0.007851434875270.4138934169710.08571468808920.387444515263-12.9538992284-9.3024583779929.1219332712
150.7043179517070.06359364017910.4379152624012.280191337560.5526689169450.545002721697-0.2861246577680.543217005522-0.00827892540817-0.4757556029270.409061807373-0.09923185254180.128226827526-0.139978645246-0.1152711579480.523179437646-0.0498856080349-0.0216136019060.3485633190880.04224076893830.2682781665615.576127770735.6883872759921.7918183769
162.09366514112.538756987840.07285175437533.106626654730.4527547994621.86174126421-0.1998185828090.174128713024-0.498263755727-0.4080603709570.250341597112-1.342725866350.03155381209420.245192628071-0.003571681086460.4263012604780.06452029557330.01063089194630.4003754442030.04157375495730.44023746336716.90801326777.2987254411125.5276472391
171.384813853630.435514823002-0.1343533339782.40984834955-0.05858051653141.95531828961-0.0284553534276-0.1186432612440.04548828619160.470234814352-0.05896949537750.325572453212-0.17007439037-0.1486030334820.09217271324090.507632113856-0.06458171237680.04034920643160.3817743368460.02121806145760.39638184563213.921014435249.067721088627.9401468811
182.013521297711.32590681236-0.6413042000752.05192539573-0.5686736223020.974294041597-0.1171132421360.144488549398-0.0353609918883-0.1247509190550.1207158257390.2797015369740.215984551246-0.290907434418-0.008472464188510.429409810552-0.0445783104063-0.03561128068790.3917949261760.04573225336110.35035104326610.706388956939.029694387513.4605925792
195.95524409459-0.781385214416-0.4261435102573.2304036475-0.4135700951473.295409576980.234884031976-0.5306307805470.5642421240880.714511054967-0.1949582147020.315651382555-0.747611180957-0.367590145667-0.005907421505920.59038706575-0.04174726731050.05775862698960.392585863721-0.08341683768790.37316265090127.233795442488.692869891421.1749723693
201.838392056240.505682735154-0.4878237358482.04983257653-0.7598927342662.041097109250.244979512684-0.2491057213450.04742419378840.475840467793-0.323340497879-0.213575318805-0.2139379094120.2520882472560.0926692942060.284417333532-0.0938968121321-0.05341527652880.3358247869110.03882216588440.3463434765940.352686216583.408178339811.6611979458
212.81719541623-2.33540791780.120115798155.058552450040.3941941109912.553384811680.172100626021-0.1909656702630.1470821899780.232800391153-0.2014401163640.1070425878320.0384365480337-0.1048246055720.05765555209950.302350892672-0.116371115997-0.04046770945990.2848701151380.01151838748020.22930675332728.329324815870.17479870411.6596709416
222.458600260042.997749531440.485538176968.632771919911.026238025711.941277231160.140308513796-0.1569178209360.540004727704-0.28523077766-0.01988740244361.11185850327-0.209158037352-0.944318053109-0.0602557133120.347246871994-0.00861865044832-0.1134008335570.6500966509710.07002043573270.69647031442616.034931384282.6944795932-2.37987862381
232.31756683507-0.686767913256-0.8825372898233.39285571846-0.4415448308392.64418336933-0.02493182333170.15623064674-0.0301054856315-0.132675718162-0.0206624063030.5758089194410.269042434797-0.6003040902640.05489130015410.289110874508-0.0600366330629-0.05190836672820.453434951261-0.009368447795030.41724155884120.645347192869.74991738560.436879244427
242.356441289792.14658209861-0.2732890921013.18468455803-0.2832305531831.1938656338-0.06370937034660.214407967818-0.326761981862-0.01276563109060.00325080052125-0.8347137557960.1933872108260.2179295356710.03870168679550.301575306920.0291400166608-0.02398658651590.3140093770440.09750257720340.44725763802648.057401747574.1827415813-0.48078034622
257.49919555522-1.07282701512-3.545312678498.048868234293.927846462963.16857848435-0.38058653612-0.45721675869-0.9385179058570.258291389342-0.04497540841560.15222338911.132419218340.05879640460360.4143181930871.05137363629-0.141458693820.05075713418540.6606215377250.05364755043930.797860348207-18.4204312479-55.132374813155.6559679117
262.925893184780.342021631340.3209008762561.865067731770.4451189735972.90818967534-0.2728143106610.150272566025-0.497153184523-0.0437910603729-0.144910247491-0.1163234349731.278959055790.7690366779310.3441052538890.9839672998950.1344913607470.2126273960040.650555862750.1518682815750.9534176108640.789105205887-54.575132836546.0296505842
271.379122920733.23135773675-1.171992693286.25932343722-2.245327345730.6861070829020.122762807448-0.222467119472-0.4100447691980.168216546201-0.547600629611-1.01451835975-0.06097238996830.3337476687550.4108648986720.75696836156-0.163848087515-0.1588779947170.6567687788140.2522168385020.77676229124635.501518796331.854621788335.8324128904
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 64 )
2X-RAY DIFFRACTION2chain 'A' and (resid 65 through 91 )
3X-RAY DIFFRACTION3chain 'A' and (resid 92 through 199 )
4X-RAY DIFFRACTION4chain 'A' and (resid 200 through 259 )
5X-RAY DIFFRACTION5chain 'A' and (resid 260 through 306 )
6X-RAY DIFFRACTION6chain 'A' and (resid 307 through 338 )
7X-RAY DIFFRACTION7chain 'A' and (resid 339 through 381 )
8X-RAY DIFFRACTION8chain 'A' and (resid 382 through 437 )
9X-RAY DIFFRACTION9chain 'B' and (resid 1 through 86 )
10X-RAY DIFFRACTION10chain 'B' and (resid 87 through 125 )
11X-RAY DIFFRACTION11chain 'B' and (resid 126 through 236 )
12X-RAY DIFFRACTION12chain 'B' and (resid 237 through 266 )
13X-RAY DIFFRACTION13chain 'B' and (resid 267 through 309 )
14X-RAY DIFFRACTION14chain 'B' and (resid 310 through 362 )
15X-RAY DIFFRACTION15chain 'B' and (resid 363 through 391 )
16X-RAY DIFFRACTION16chain 'B' and (resid 392 through 430 )
17X-RAY DIFFRACTION17chain 'C' and (resid 1 through 273 )
18X-RAY DIFFRACTION18chain 'C' and (resid 274 through 438 )
19X-RAY DIFFRACTION19chain 'D' and (resid 1 through 86 )
20X-RAY DIFFRACTION20chain 'D' and (resid 87 through 236 )
21X-RAY DIFFRACTION21chain 'D' and (resid 237 through 271 )
22X-RAY DIFFRACTION22chain 'D' and (resid 272 through 294 )
23X-RAY DIFFRACTION23chain 'D' and (resid 295 through 363 )
24X-RAY DIFFRACTION24chain 'D' and (resid 364 through 431 )
25X-RAY DIFFRACTION25chain 'E' and (resid 6 through 23 )
26X-RAY DIFFRACTION26chain 'E' and (resid 24 through 46 )
27X-RAY DIFFRACTION27chain 'E' and (resid 47 through 140 )

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