THERE IS ONE COMPLEX IN THE ASYMMETRIC UNIT, WHICH CONSISTS OF TWO ALPHA-BETA TUBULIN HETERODIMERS ...THERE IS ONE COMPLEX IN THE ASYMMETRIC UNIT, WHICH CONSISTS OF TWO ALPHA-BETA TUBULIN HETERODIMERS (CHAINS A-B AND C-D), AND ONE STATHMIN-LIKE DOMAIN OF RB3 (RB3-SLD) WHICH CORRESPONDS TO STAHMIN RESIDUES 5 TO 145 WITH THE ADDITION OF ONE ACETYLATED ALANINE AT THE N-TERMINUS. THE NUMBERING OF RB3-SLD IS ACCORDING TO THE STATHMIN SEQUENCE. ALPHA-TUBULIN AND BETA-TUBULIN HAVE BEEN ALIGNED AS IN NOGALES ET AL., NATURE VOL 391,199-203. IN THIS ALIGNMENT, RESIDUES 45-46 AND 361-368 OF ALPHA-TUBULIN ARE MISSING IN BETA-TUBULIN. AS THE SEQUENCE OF OVIS ARIES(SHEEP) TUBULIN IS NOT AVAILABLE, THE BOS TAURUS TUBULIN SEQUENCES (ALPHA: ISOTYPE 1A, GI:194666935, BETA: ISOTYPE 2, GI:51491829) WERE USED AS A REFERENCE BUT FOR THE ILE TO VAL SUBSTITUTION AT POSITION 318 ON BETA TUBULIN. THIS IS BASED ON DIFFERENCES BETWEEN TUBULIN ISOTYPES AND ON THE RELATIVE EXPRESSION ON THESE ISOTYPES IN MAMMALIAN BRAIN.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.86 Å3/Da / Density % sol: 68 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG, PIPES BUFFER, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K
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