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Open data
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Basic information
Entry | Database: PDB / ID: 6tiu | ||||||
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Title | DROSOPHILA GTP-TUBULIN Y222F MUTANT | ||||||
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![]() | CELL CYCLE / MICROTUBULE / MICROTUBULE DYNAMICS / MICROTULE NUCLEATION | ||||||
Function / homology | ![]() HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / COPI-mediated anterograde transport / COPI-independent Golgi-to-ER retrograde traffic / COPI-dependent Golgi-to-ER retrograde traffic / Kinesins / astral microtubule / Neutrophil degranulation / lysosome localization / microtubule depolymerization / regulation of microtubule polymerization or depolymerization ...HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / COPI-mediated anterograde transport / COPI-independent Golgi-to-ER retrograde traffic / COPI-dependent Golgi-to-ER retrograde traffic / Kinesins / astral microtubule / Neutrophil degranulation / lysosome localization / microtubule depolymerization / regulation of microtubule polymerization or depolymerization / tubulin binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / structural constituent of cytoskeleton / microtubule cytoskeleton organization / spindle / neuron projection development / mitotic cell cycle / growth cone / microtubule / hydrolase activity / neuron projection / GTPase activity / centrosome / GTP binding / perinuclear region of cytoplasm / Golgi apparatus / nucleus / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gigant, B. | ||||||
![]() | ![]() Title: GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation. Authors: Ayukawa, R. / Iwata, S. / Imai, H. / Kamimura, S. / Hayashi, M. / Ngo, K.X. / Minoura, I. / Uchimura, S. / Makino, T. / Shirouzu, M. / Shigematsu, H. / Sekimoto, K. / Gigant, B. / Muto, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 743.9 KB | Display | ![]() |
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PDB format | ![]() | 620.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.7 MB | Display | |
Data in XML | ![]() | 63.8 KB | Display | |
Data in CIF | ![]() | 86.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6tisC ![]() 6tiyC ![]() 6tizC ![]() 3rycS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 3 types, 5 molecules ACBDE
#1: Protein | Mass: 49989.266 Da / Num. of mol.: 2 / Mutation: K40R Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 50178.137 Da / Num. of mol.: 2 / Mutation: Y222F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Protein | | Mass: 16851.133 Da / Num. of mol.: 1 / Mutation: S4A, C14A, F20W Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Non-polymers , 4 types, 14 molecules ![](data/chem/img/GTP.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/GDP.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/GDP.gif)
#4: Chemical | ChemComp-GTP / #5: Chemical | ChemComp-MG / #6: Chemical | ChemComp-SO4 / #7: Chemical | ChemComp-GDP / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.67 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / Details: PEG1500, LISO4, PIPES BUFFER, PH 6.80 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Nov 28, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 3.57→48 Å / Num. obs: 25304 / % possible obs: 98.7 % / Redundancy: 11.7 % / CC1/2: 0.997 / Net I/σ(I): 4.15 |
Reflection shell | Resolution: 3.57→3.79 Å / Mean I/σ(I) obs: 0.92 / Num. unique obs: 3723 / CC1/2: 0.565 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3RYC Resolution: 3.571→48 Å / Cor.coef. Fo:Fc: 0.872 / Cor.coef. Fo:Fc free: 0.862 / Cross valid method: THROUGHOUT / SU Rfree Blow DPI: 0.655
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Displacement parameters | Biso mean: 110.93 Å2
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Refine analyze | Luzzati coordinate error obs: 0.6 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.571→48 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.571→3.611 Å
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Refinement TLS params. | T11: 1.5199 Å2 / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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